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کامل مرشد دی پہچان

کامل مُرشد دی پہچان

پھڑ دامن پیر منا لَے توں
اِنج سوکھا ای رب نوں پا لَے توں
رکھ پہلی پوڑی پیر میاں
انج کردا جا توں سیر میاں
تیری ہر دم ہووے خیر میاں
من اپنے نوں سمجھا لَے توں
پھڑ دامن پیر منا لَے توں
پھڑ مرشد کر انکار نہیں
بنِ مرشد بیڑا پار نہیں
لینی ہور کسے وی سار نہیں
اُس در تے ڈیرے لا لَے توں
پھڑ دامن پیر منا لَے توں
اوہ بستی یار مدینہ ہے
اوتھے ملدا نور خزینہ ہے
اوتھے ہوندا ٹھنڈا سینہ ہے
کر ہمت درشن پا لَے توں
پھڑ دامن پیر منا لَے توں

سوہنا مرشد ماڑی والا اے
جس کیتا نور اجالا اے
سدا وسن جھوکاں شالا اے
اُس در توں فیض کما لَے توں
پھڑ دامن پیر منا لَے توں

ایہو طرز طریقہ سارا جے
ایہو سب توں راز نیارا جے
اُس رب دا نام پیارا جے
اُس نام دا ورد پکا لَے توں
پھڑ دامن پیر منا لَے توں

ڈِٹھا خواجہ عبد رحمن میاں
ہِن دُھماں وچ جہان میاں
جتھے جھکدے شاہ سلطان میاں
اُس در توں فقر کما لَے توں
پھڑ دامن پیر منا لَے توں

اوہ مٹھا ہے خلق طبیعت دا
پابند اے بڑا شریعت دا
اوہ اچھا ہے ذہنیت دا
اوہدے در تے کلّی پا لَے توں
پھڑ دامن پیر منا لَے توں

اوہدا میتھوں سن توں حالا نی
اوہدا بہتا شان نرالا نی
اوہدا وارث کملی والا نی
اُس در تے جوگ کما لَے توں
پھڑ دامن پیر منا لَے توں

وچ ماڑی جھوکاں لائیاں نیں
اوتھے ذکری جلیاں پائیاں نیں
گیاں ہر جاء ہو...

صفات الخطباء و مراعاة السامعين لتامين السلام في الجمتمعات الاسلامية

The religious sermon and the speaker play a keyrole in the Islamic societies in shaping and orienting the opinions, beliefs, approach and behavior of the masses. Addressing people is a great responsibility, for which the speakers will be held accountable to Allāh Almighty. Therefore, a speaker of Islām must be equipped with sound knowledge of Islām and well acquainted with the present time, too. Moreover, he must possesstherequired temperament of tolerance, compassion and consideration, while speaking to his audience. The example of the Holy Prophet Muḥammad(r) provides a complete model of conduct for today’s speakers. We notice that audiences are moved with the eloquence of the speakers. A careless speaker may drive his audience into the abyss of disappointment, alienation from Islām, terrorism andextremism, etc. ; On the other hand, he can alleviate their ignorance, reshape them to become the desired peaceful Muslims through his power of speech and influence of his character. In this paper, the author, tried to make the Islamic speakers realize their duties in respect of their audience in the light of the Sunnah of the Prophet(r).

Microbial Strain Development for Hyperproduction of Β–Glucosidase Through Γ–Rays Mutagenesis and Expression Cloning

The cellulose enzymatic hydrolysis is carried out by the synergistic action of cellulase enzyme complex, namely endo-glucanase, exo-glucanase and β-glucosidase (BGL). BGL is responsible for the regulation of the cellulolytic process as both endo- and exo-glucanase activities are often inhibited by cellobiose. In recent years, interest in BGL has gained momentum, owing to its potential use in various biotechnological processes including biomass degradation, fuel ethanol production, release of aromatic residues, extraction of fruit juices, production of fermented foods etc. A. niger is by far the most efficient BGL producer among the microorganisms investigated. To cope with the current industrial demand it is necessary to produce BGL with improved catalytic and kinetic properties. The current report deals with the development of A. niger strain through gamma rays treatment. Hyper producer mutant M-6 was selected on the basis of 2-deoxy-D-glucose resistance, which showed about two fold higher production of BGL as compared to control. Both the parent and mutant had shown best results with 3% wheat bran at 30 °C and pH 5. Random mutagenesis resulted in mutations within the BGL gene sequence, leading to overall conformational change in BGL secondary structure. The subunit and native molecular mass of the BGLs from parent and mutant were same i.e. 130 and 252 kDa, respectively. LC-MS/MS analysis also confirmed the identity of protein bands as BGL from both strains with a slight difference in molecular masses. The mutant BGL proved to be 1.6 fold efficient for cellobiose hydrolysis with lower activation energy (Ea) and thermodynamically more stable against denaturants than parent BGL. The mutant BGL gene was also expressed in Pichia pastoris and the recombinant BGL possessed similar characteristics as that of the mutant BGL. Effect of metals on parent and mutant BGLs showed that Ca2+ decreased the Ea, resulting in efficient formation of ES*-complex as compared to apo-BGL, while, Mn2+ decreased the Ea at lower metal concentrations only. The heat of ionization for the acidic and basic limbs of the apo- BGL reduced significantly in the presence of Ca2+ and Mn2+. The turnover (kcat) of apo-BGL showed an increase in the presence of low concentrations of Ca2+ and Mn2+. The BGL activation mechanism by Ca2+ was of mixed nature where Ca2+ was preferably binding with free enzyme, while Mn2+ was preferably binding with ES*-complex rather than free enzyme (partially un- competitive). Ca2+- and Mn2+- bound BGL also showed higher thermo-stability as compared to apo-BGL. Immobilization of BGL through entrapment on latex and silicone resulted in an increase in optimum temperature and thermal stability than the free enzyme. The immobilized BGL also increased the cellulose hydrolysis rate with more than 70% functional stability after multiple reuses. The current study has resulted in a highly stable and catalytically active BGL enzyme which may have substantial applications in biofuel and biorefinery industries.
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