Search or add a thesis

Advanced Search (Beta)
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

پروفیسرحامد حسن قادری

پروفیسر حامد حسن قادری
افسوس ہے کہ ادھر چند مہینوں کے اندر دنیائے علم و ادب کی کئی نامور ہستیوں، پروفیسر حامد حسن صاحب قادری، سید ہاشمی صاحب فرید آبادی اور مولانا صلاح الدین احمد نے انتقال کیا، پروفیسر حامد حسن صاحب قادری ہماری پرانی علمی بزم کی یادگار تھے، اردو اور فارسی زبان و ادب اور اس کی تاریخ پر ان کی نظر بڑی گہری اور وسیع تھی، تاریخ داستان اردو ان کی وسعت نظر کی شاہد ہے، وہ عرصہ تک سینٹ جانس کالج آگرہ میں اردو اور فارسی کے استاد رہے، ریٹائر ہونے کے بعد کراچی چلے گئے تھے، اور وہیں وفات پائی، ان کی وفات سے ایک پرانی علمی و تہذیبی یادگار مٹ گئی۔
(شاہ معین الدین ندوی، اگست ۱۹۶۴ء)

علم الفواصل کی تاریخ، حکمت اور طرق معرفت کا تحقیقی جائزہ

Elm-ul-Fawasil (Intespace) is the knowledge of the leading verses of the Quran. To inquire the numer of verses, interspace is the main objective of this knowledge. We have so far considered the miraculous characteristics of the Quran from the scientific and historical points of view. In addition to this, the Quran aslo possesses an astounding, inimitable style from the literacy perspective. The words employed in the Quran are exceedingly special, both in terms of meaning and also of fluency and effect style. Quran is the ultimate miracle of God. The present Quran is in the some condition as it was revealed because almighty Allah has been taken the responsibility its protection. This paper present the definition of Elm-ul-Fawasil, Histoy, etymology, title, its types and the importance of this knowledge.

Production and Characterization of Manganese Peroxidase Mnp from Mutant and Native Strains of Coriolus Versicolor

Economical production of industrial enzymes in higher yields has been an area of intensive research interest for the last many decades. In recent years, scientists have made tremendous progress in enzyme biotechnology that has opened up new opportunities for enzymes in molecular biology as well as industrial applications. Fungi belong to a group of organisms with the ability to produce different types of enzymes. The importance of fungi is not limited to their native products; they are also useful in the development and commercialization of new products through the modern techniques of mutagenesis and molecular biology. Enzyme production in higher yields is important for any biomedical or industrial application of enzymes. White rot fungi are efficient lignin degrading microorganisms that produce high activities of ligninolytic enzymes, including lignin peroxidase, manganese peroxidase and laccase. Manganese peroxidase has got tremendous applications in biomass utilization, bioremediation, pulp and paper industry, food technology, nanobiotechnology and medicine. With increasing new biotechnological and industrial applications of manganese peroxidase there is dire need to search for new and hyperproduccing enzyme sources. The various techniques for developing hyperproducing fungal strains are radiation and chemical mutagenesis, and genetic engineering. Chemical mutagenesis offers the possibility of a wider and more economical technique for developing mutant strains for producing biocatalysts in industry, waste treatment, medicine, and in the development of bioprocess monitoring devices like biosensors. Keeping in view the above mentioned facts, a study was conducted to develop a hyperproducing mutants of Coriolus versicolor IBL-04 through chemical mutagenesis. Two chemical mutagens ethedium bromide (EB) and ethyle methansuphonate (EMS) were used for mutational work and hyperprucing mutants in each case were selected on basis of MnP production in solid state fermentation. The physical and nutritional parameters for the production of MnP by native and selected mutants were optimized through one-factor-at-a-time optimization stratergy. Physico-chemical culture conditions, such as pH, tempature, moisture levels, inoculum size, C:N ratio, mediators and metal ions were optimized and maximum MnP activities by mutant strains EB-60 and EMS-90 were 2796 U mL -1 and 3140 U mL -1 , repectivelty, as compared to 1635 U mL -1 MnP actvity from native strain. An increase of 3.2-fold in enzyme production after mutagenesis and optimization of various parameters was achieved as compared to native strain. The MnPs produced by native and mutant strains were 1.4-5.5 fold purified through ammonium sulphate precipitation, ion exchangechromatography and gel filtration. The purified MnPs from from mutant strains had the molecular masses of around 43 kDa on SDS-PAGE. The enzymes from native , EB-60 mutant and EMS-90 mutant strains of Coriolus versicolor were compared in terms of their pH & temperature optima and kinetic characteristics. The lower K M and higher V max values from selected mutant strains suggested that the MnPs from mutants were more efficient and stable as compared to MnP from the native strain. The results of this work demostrated that the random chemical mutagenesis of Coriolus versicolor IBL-04 significantly enhanced MnP production under optimized laboratory conditions. Higher activities and thermo-stabilities of MnPs from mutant strains suggest the potential of mutant strains for commercial scale MnP production for diverse industrial applications.
Author