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Social Media Discourse and Public Opinion

Thesis Info

Author

Ammara Shafique

Supervisor

Muhammad Aslam

Program

MS

Institute

Riphah International University

Institute Type

Private

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Page

vi, 72 . ; 30 cm. +CD : ill.

Language

English

Other

Submitted in fulfillment of the requirements for the degree of Master of Philosophy in English (Linguistics); Includes bibliographical references; Thesis (MS-English literature)--Riphah International University, 2018; English; Call No: AMM 302.231

Added

2021-02-17 19:49:13

Modified

2023-01-08 02:11:16

ARI ID

1676711766334

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غیر مطبوعہ کتب

غیر مطبوعہ کتب

                ناطق ایک انوکھا ادیب ہے،جس کا شمار ان عصری ادب کے ادیبو ں میں ہوتاہے جنہوں نے اپنے عصری دور کو اپنے متاثر کن کام سے حیران کیا ہے،جو تہذیب اور تاریخ کی ایسی منظر کشی کرتے ہیں کہ پڑھنے والا دنگ رہ جاتا ہے۔اردو ادب میں ناطق کا کام حیرت زدہ کرنے والا ہے۔کیونکہ وہ حقیت اور کہانی کے پیچیدہ پہلوؤں کو لے کر سامنے آتا ہے۔ان کی غیر مطبوعہ کتب درج ذیل ہیں:’’کوفے کا مسافر(ناول)‘‘ناطق نے اپنے لکھنے کا آغاز شاعری سے کیا، نظم لکھنا سب سے پہلے شروع کی۔ان کی ادبی تخلیقات کا مختصراًجائزہ کچھ یوں ہے۔

ابن خرداذبہ اور ان كى كتاب المسالك والممالك: تاریخى وتنقیدى جائزہ

Ibn Khurdathba was a man with variable culture, he had an important role in the science of geography especially (knowing of the roads, locations of the cities, weather in addition to the news of general history and some arts like (music and singing )  The growth of Ibn Khurdathba in a family worked in the military field, close to the royal court has helped him to start his practical life early and allowed him also to contact with caliphs, ministers and writers in addition to occupying of the significant positions in the state, making it easier to get information from their original resources. Ibn Khurdathba has descended from Persian family lately entered into Islam۔ One of earliest geographical works of this period, his famous Kitab al- Masalik wa’l= Mamalik’. It gives a summary of the main trade routes of the Arab world and in addition provides description of such distant China, Korea and Japan. This article attempts his life and his “Al masalik wa al mamalik” book, varies sides.

Expression Profiling of Reporter Genes Induced by Rice Polyphenol Oxidase Promoter in Transgenic Model Plant

Polyphenol oxidase (PPO) enzymes are ubiquitous in plant kingdom that catalyzes the oxidation of phenols to highly reactive quinones. PPO plays important role in plant defense mechanisms against biotic and abiotic stresses but the regulation of PPO promoter in response to stresses remains unclear. Here, Oryza sativa polyphenol oxidase (OsPPO) promoter was fused separately to β-glucuronidase (GUS) and firefly Luciferase (LUC) reporter genes. Agrobacterium strain GV3101 harbouring OsPPOLUC and OsPPOGUS (separate clones) was used to transform Arabidopsis by floral dip method. T1 transgenic seeds were used to obtain T2 lines which were finally checked for the effects of wounding, ABA, MJA applications and drought as well as salt stress on PPO regulation. Expressional profiling of OsPPO promoter by real- time (RT-PCR) in transgenic Arabidopsis has revealed a higher level (11 folds) of expression by wounding. However, OsPPO promoter was also expressed in response to ABA by an induction of 3 fold but it was not induced by MJA. ABA plant hormone which regulates key processes biotic and abiotic stresses ultimately triggering plant defense related genes. ABA and wound inducibility of OsPPO promoter is a strong indicative of its role in plant defense mechanism against biotic and abiotic stresses. Moreover, transgenic T2 plants were also screened against different concentrations of osmotic stress (PEG-6000) and salt (NaCl). Experimental data showed that relative GUS expression of OsPPO gene promoter increased with the increase of osmotic stress. In case of salt stress, OsPPO induction showed similar trend and GUS expression was increased as salt concentration increased. This response of OsPPO to drought and salt stress suggest the x xpossible participation of PPO in plants defense against drought as well as salt stress. Moreover, OsPPO promoter sequence was computationally analyzed by Signal Scan (PLACE), which indicated the presence of different cis- regulatory elements, for example wound responsive elements, ABA signaling complexes responsive to drought and salt responsive regulatory elements. During in vivo experimental work, the induction and expression of reporter genes proved the presence and functioning of these cis-regulatory elements. In summary the role of OsPPO in wounding, ABA application, drought and salt stresses, and the subsequent OsPPO promoter induction indicates that these stresses induce PPO expression in plants as a defense mechanism.