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Studies on High Molecular Weight Glutenin Sub-Units Polymorphism in Wheat

Thesis Info

Author

Khan Muhammad Asghar

Department

Deptt. of Biological Sciences, QAU.

Program

Mphil

Institute

Quaid-i-Azam University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2000

Thesis Completion Status

Completed

Page

76

Subject

Biological Sciences

Language

English

Other

Call No: DISS/M.Phil BIO/861

Added

2021-02-17 19:49:13

Modified

2023-02-19 12:33:56

ARI ID

1676716614152

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اشاریہ سازی کی اولین کاوش

یونیورسٹی آف سیالکوٹ کے شعبہ اردو کو یہ اعزاز حاصل ہے کہ اس میں ایم ایس سطح کے تحقیقی مقالات کے لیے جدید اور متنوع موضوعات پر کام کروایا جا رہا ہے۔ روزینہ یاسمین  نے تحقیقی جرائد " تحصیل " اور "مآخذ"کی اشاریہ سازی کا کام جس محنت اور شوق سے کیا اس کے لیے یہ مبارکباد کی حق دار ہیں۔ طالبہ کی اس اولین کاوش کو تمام اساتذہ کرام نے بھی خوب  سراہا ۔یہ ہماری خوش نصیبی کہ ہمیں اردو ادب کے نامور محقق،نقاد، مصنف ، استاد اور تحقیقی جریدہ "تحصیل" کے مدیرپروفیسرڈاکٹر معین الدین عقیل صاحب کی معاونت اور قیمتی مشورے میسر آئے۔ایسی عظیم شخصیات اردو ادب کا سرمایہ افتخار ہیں ۔ ڈاکٹر صاحب نوجوان محققین کی نہ صرف حوصلہ افزائی کرتے ہیں بلکہ ان کی ہر ممکن کوشش بھی کرتے ہیں۔

تحقیق میں نئے موضوعات پر کام کروانا وقت کی ضرورت ہے۔ جن جامعات نے اشاریہ سازی پر تحقیقی کام کروایا ان میں پنجاب یونیورسٹی(لاہور)، بہاء الدین زکریا یونیورسٹی(ملتان)، جامعہ کراچی (کراچی)، جامعہ پشاور (پشاور)، اسلامیہ یونیورسٹی(بہاول پور)، انٹرنیشنل اسلامک یونیورسٹی (اسلام آباد) ، نمل یونیورسٹی(اسلام آباد)اورعلامہ اقبال اوپن یونیورسٹی (اسلام آباد) وغیرہ کی کاوشیں قابل تحسین  ہیں ۔

اردو تحقیق کو جدید تناظر میں دیکھیں تو اشاریہ سازی کواساسی حیثیت دی جاتی ہے کیونکہ محققین کے لیے سب سے بڑا مسئلہ کم وقت میں مطلوبہ مواد تک رسائی کا ہوتاہے اور اشاریوں کی مدد سے آپ کم وقت اور کم محنت سے آسانی...

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The Qur'an has innumerable features. Its teachings serve as blessing and guidancefor the whole mankind. It provides complete code oflifefor man. It catersfor both life in the present world and that in the hereafter. The Qur'an is regarded as inimitable due to the accuracy ofscientific information presented 1400 years ago and only recently proven or discovered, as well as for its unprecedented Arabic literary style of highest standard. This article discusses extraordinary features of the Qur'an as explained and highlighted by majority of commentators of the Qur’an in their commentaries of the Qur'an.

Synthesis and Characterization of Polymers/Ferrite Nano-Composites to Enhance Their Application in Biomedical Field

Multidimensional magnetic cobalt ferrite (CoFe2O4) nanoparticles (NPs) were synthesized by a co-precipitation method under the effect of three capping agents called sorbitol, tergitol-1x (T-1x) and didecyldimethyl ammonium bromide (DDAB). The synthesized cobalt ferrite nanoparticles (CFNPs) were functionalized with guar gum (GG), xanthan gum (XG), gum arabic (GA), polymethacrylic acid (PMAA) and were subsequently characterised by Fourier transform-infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), X-ray diffraction (XRD) and contact angle studies. Vibrating sample magnetometry (VSM) was used for magnetic measurements of the native and polymers-coated CFNPs. The microstructural morphology of the uncoated and polymers-coated CFNPs was established using scanning electron microscopy (SEM), Transmission electron microscopy (TEM), atomic force microscopy (AFM) and dynamic light scattering (DLS) studies. The antibacterial activity of native and coated CFNPs was also tested against Escherichia coli and Staphylococcus aureus by using disc diffusion method. Furthermore, the interaction of cobalt ferrite nanoparticles with calf-thymus DNA was investigated and characterized by FT-IR. The biocompatibility of naked and coated CFNPs was also examined against normal (CHO) and cancerous (Huh-7) cell lines by performing MTT assay. Finally, the doxorubicin release profile from the drug-loaded functionalised CFNPs in the presence of an externally applied magnetic field was evaluated using UV-visible spectroscopy. The results show that the DDAB is more effective capping agent to control the particle size and dispersion of CFNPs in aqueous medium. Antibacterial activity test and MTT assay exhibited that coated-CFNPs are significantly more biocompatible as compared to native CFNPs. Polymer coating decreased the contact angle of the native CFNPs from 92° to 40°, which indicated that it modified the CFNPs surface from hydrophobic to hydrophilic. VSM analysis demonstrated that polymers coated cobalt ferrite NPs (CoFe2O4.NPs@polymers) also retained the magnetic characteristics of the bare cobalt ferrite NPs (Ms ∼77.2 emu/g), endorsing their application as promising magnetic nanovectors (MNV). The synthesised drug loaded-polymer coated cobalt ferrite NPs, (CoFe2O4.NPs@polymers-Doxo) exhibited significantly higher controlled discharge of doxorubicin at acidic pH (5.0) than at neutral pH (7.4). In vitro cytotoxic studies confirmed the cytocompatibility mode of coated CFNPs against Chinese Hamster Ovary and Huh-7 cell line, while 0.2 mg.mL1- dose of drug loaded magnetic nanocarriers inhibited the cell viability of Huh-7 up to 60%. These results strongly encourage the utilization of biocompatible magnetic nanocarriers (CoFe2O4.NPs@polymers) in targeted drug delivery territory for doxorubicin.