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Home > Biochemical and Molecular Characterization of Vibrio Spp from Diverse Sources and Comparative Genome Analysis of Type Vi Secretion System

Biochemical and Molecular Characterization of Vibrio Spp from Diverse Sources and Comparative Genome Analysis of Type Vi Secretion System

Thesis Info

Author

Sobia Shaukat

Supervisor

Abdul Rauf Siddiqi

Department

Department of Biosciences

Program

RMI

Institute

COMSATS University Islamabad

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Biosciences

Language

English

Added

2021-02-17 19:49:13

Modified

2023-01-06 19:20:37

ARI ID

1676719655444

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ایناں ظلم جناب نئیں بن دا

اینا ظلم جناب نئیں بن دا
عشق چہ انج حجاب نئیں بن دا

درداں دی ایہہ دین اے ساری
اینویں شعر کتاب نئیں بن دا

جے کر نیت کھوٹی ہووے
چنگا کم ثواب نئیں بن دا

ورہیاں قید اے کٹنی پیندی
قطرہ انج شراب نئیں بن دا

رات معراج دی ہے وے ڈھولا
کرنا اج نقاب نئیں بن دا

جے ایہہ اکھ نہ پانی دیندی
دھرتی تے پنجاب نئیں بن دا

جنے دکھ میں جر لئے ربا
میرا ہُن حساب نئیں بن دا

Role of Islam in Practical Life amongst Some Young Swiss Muslim Adults: A Focused Ethnographic Analysis

Contemporary secular Switzerland has been polarised by more than two decades of anti-Muslim sentiments, conveyed through misrepresentative narratives by some politicians and media, ‘otherising’ Islam and Muslim-related issues. This polarised environment may have triggered ambivalent impacts on the personal development of Swiss-born Muslims Consequently, I suggest that they live in an ambivalent position, where their status as Muslim is not fully acknowledged and their belonging to Switzerland still questioned. Indeed, this article will explore the following research question: How do some young Swiss-born Muslim adults construct their subjectivities and experiences linked to Islam in their daily lives in contemporary Switzerland? The research methodology of this focused ethnography draws on various semi-structured interviews and informal conversations with several young Swiss Muslim men and women. The paper focuses on (a) Switzerland’s socio-cultural and political context and (b) the various ways this study’s research partners construct their ambivalent subjectivities through the analysis of their individual trajectories and narratives. It concludes that young Swiss Muslim adults formulate and produce alternative narratives, in order to make sense of their lives and accommodate various layers of identification in contemporary Switzerland. Eventually some recommendations for further research are formulated.  

Isolation, Identification and Encapsulation of Probiotics from Graps/Spinach and Biological Screening of Their Secondary Metabolites

Probiotics are living microorganisms that confers beneficial effects when taken in adequate amount. Probiotic are the organisms which possess beneficial effect when ingested by the host. Lactobacilli are an extremely important group of probiotic bacteria. The aim of this study is to isolate lactobacillus strains from Vitis vinifera and Spinacia oleracea with de Man, Rogosa and Sharpe (MRS) broth to search for a new effective probiotic strains. Various strains of Lactobacilli identified and analysed for their probiotic properties including acid, salt tolerance and different fermentative test. The test sample showed significant activity against Escherichia coli (88%) Salmonella typhi (85%) and Staphylococcus aureus (84%). The test sample showed good (60%) phytotoxic activity against Lemna minor at 1000 µg/ml. To protect the isolated Lactobacillus strains encapsulation was done in the product’s environment to improve their survival. The results obtained from the different fractions of metabolites were selected for in vivo acute toxicity studies. The toxicological study revealed that the test samples are safe for animal use upto 800 mg/kg. The test sample showed dose dependent anti-nociceptive potentials, the significant effect was observed with 500 and 800 mg/kg b.w dose. The analgesic effect of diclofenac sodium (standard drug) was greater than test sample. The significant (P < 0.01) anti-nociceptive potentials was noticed at 800 mg/kg b.w. of the animal for normal saline (35.50 ± 0.84), chloroform (29.67 ± 1.22), ethyl acetate (30.67 ± 1.22) and (29.00 ± 0.93) chloroform fraction of L. plantarum secondary metabolites. The test sample reduced the motility of GIT in mice. The reduction in GIT motility is dose dependent and maximum effect was produced by 800 mg/kg dose. The test sample caused different degree of movement of charcoal meal in the intestine i.e. 24.17, 20.17 and 17.83cm at the tested doses of 200, 500 and 800 mg/kg, respectively. During isolation and characterization of chemical constituents (2-(3,4-dihydroxyphenyl)-3,5,7trihydroxy-4H-chromen-4-one),(1,2,3,4,5,6-Hexanehexol), (2-(3,4-dimetoxyphenyl)-5,7 dihydroxy-6-metoxy-4H chromen-4-one),(5-hydroxy-7-methoxy-2-(4-methoxyphenyl) 4H-chromen-4-one), (3S,3aS,5aR,9bS)-3,5a,9-trimethyl-3a,4,5,5a,7,8 hexahydronaphtho [1,2-b]furan 2,6(3H,9bH)-dione. Further investigations would help in the suitability of this isolate for human consumption and domestic animals feeds formulations as important probiotic.