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Simultaneous Production of Biomass and Extracellular Enzymes by Growing Filamenous Fungi on Agricultural and Industrial Wastes

Thesis Info

Author

Nadeem Nawazish Malik

Department

Faculty of Science,Institute of Chemistry Studies

Program

PhD

Institute

University of the Punjab

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

1987

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Added

2021-02-17 19:49:13

Modified

2023-01-06 19:20:37

ARI ID

1676729002691

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ورفعنا کی صدا (منظوم)

ورفعنا کی صدا
محمد اویس ازہر مدنی
عشقِ احمدؐ کی ضیا ہے ورفعنا کی صدا
دلِ عرفاں کی صدا ہے ورفعنا کی صدا
قدسیوں کی بھی ندا ہے ورفعنا کی صدا
سرورِ دیںؐ کی ثنا ہے ورفعنا کی صدا
کس کو معلوم ہوئی رفعتِ سرکارِ جناںؐ
کون جانے گا کہ کیا ہے ورفعنا کی صدا
پڑھ کے ملتا ہے سکونِ دل و جاں قاری کو
قلبِ عاشق کی نوا ہے ورفعنا کی صدا
مدحتِ سرورِ عالمؐ ہے ہر اک پر لازم
اک فریضے کی ادا ہے ورفعنا کی صدا
جاں گزیں کرتی ہے دل میں شہِ دیںؐ کی الفت
مرے آقاؐ کی ولا ہے ورفعنا کی صدا
خود سے اک لفظ بھی لکھا نہیں جا سکتا تھا
مرے مولیٰ کی عطا ہے ورفعنا کی صدا
بخش دیتی ہے تر و تازگی ایمان کو بھی
خلد کی آب و ہوا ہے ورفعنا کی صدا
ہے یہ گنجینئہ عشقِ شہِ والاؐ ازہر
بالیقیں دولتِ ما ہے ورفعنا کی صدا

PENINGKATAN KUALITAS PEMBELAJARAN MENYIMAK CERPEN DENGAN MENGGUNAKAN MEDIA REKAMAN PEMBACAAN CERPEN PADA SISWA KELAS XI IPA2 SMA NEGERI 1 BONTOTIRO KABUPATEN BULUKUMBA

Improving the Quality of Learning to Listen to Short Stories by Using Recorded Media for Reading Short Stories for Class XI IPA2 Students of SMA Negeri 1 Bontotiro, Bulukumba Regency.” This study aims to describe the improvement in the quality of learning to listen to short stories using short story reading recording media for students of class XI IPA2 SMA Negeri 1 Bontotiro, Bulukumba Regency.             The results of the study prove that improving the quality of learning to listen to short stories using short story reading recording media in class XI IPA2 SMA Negeri 1 Bontotiro Bulukumba Regency at the planning stage found an increase in the ability of teachers in the field of study to plan better learning implementation in cycle II. In the implementation stage, there was an increase in student activity during the learning process, such as the sincerity, discipline, and self-confidence of students following the learning process. The evaluation stage found an increase in the results of the short story listening test, showing that in the first cycle 56.09% of students experienced mastery learning, and in the second cycle it reached 97.56% who experienced learning mastery. Based on the results of the study, it was concluded that the recording media for reading short stories could improve the quality of learning to listen to short stories in class XI IPA2 SMA Negeri 1 Bontotiro, Bulukumba Regency

Biological Control of Damping off Pythium Debaryanum Diseases of Chilies

Cardiovascular complications, such as myocardial infarction, atherosclerosis, cardiomyopathy and heart failure are amongst the major causes of morbidity and mortality among diabetic patients. Diabetic cardiomyopathy (DCM) is a progressive disease of the heart muscle mediated by hyperglycemia and oxidative stress. Endothelial progenitor cells (EPCs) have shown promising potential to repair the diabetes induced cardiac damage. EPCs differentiate into functional and mature endothelial cells. However, challenges such as deteriorated functional abilities and survival of EPCs derived from diabetic subjects limit the possible outcome of autologous EPCs based therapies. Diazoxide (DZ), a highly selective mito-KATP channel opener, has been shown to improve the ability of mesenchymal stem cells and skeletal myoblasts for the repair of damage to heart muscles. In the present study, effect of DZ preconditioning was evaluated in determining the ability of diabetes affected bone marrow-derived EPCs to repair streptozotocin (STZ) induced DCM in rats. Diabetic EPCs (DM-EPCs) were characterized by immunocytochemistry, flow cytometry and reverse transcriptase PCR for endothelial markers: vWF, VE Cadherin, VEGFR2, PECAM, CD34 and eNOS. Preconditioning was performed in vitro, by incubation of DM-EPCs in 200 μM DZ for 30 minutes, followed by H2O2 induced oxidative stress and hyperglycemic stress. Oxidative stress to preconditioned and non preconditioned DM-EPCs was induced by exposure to 200 μM H2O2 for 2 hours whereas for hyperglycemic stress induction, the cells were exposed to 30 mM glucose media. Non-preconditioned EPCs with and without exposure to H2O2 were served as controls. IX The treated cells were evaluated for survival (XTT cell viability assay), senescence, paracrine potential (by ELISA for VEGF) and alteration in gene expression (VEGF, SDF-1α, HGF, bFGF, Bcl2 and caspase-3). DZ preconditioning of DM-EPCs significantly increased survival accompanied by enhanced release of VEGF and reduced senescence in DZ DM-EPCs+H2O2 group as compared to DM EPCs+H2O2 group under oxidative and hyperglycemic stress conditions. Furthermore, DZ preconditioned DM-EPCs exhibited upregulated expression of prosurvival genes under oxidative stress (VEGF, SDF-1α, HGF, bFGF and Bcl2) and under hyperglycemic stress (VEGF and Bcl2) while down regulated the expression of Caspase-3 in DZ DM-EPCs+H2O2 group as compared to DM-EPCs+H2O2 group under oxidative and hyperglycemic stress conditions as determined by reverse transcriptase PCR and real time PCR. For in vivo studies, diabetes was induced in Wistar rats by injecting 40 mg/kg streptozotocin. After eight weeks, 2x106/70 μl of serum free medium, each of DZ preconditioned DM-EPCs and non-preconditioned DM-EPCs were transplanted into the left ventricle (LV) of diabetic rats (n꞊6 rats per group). After four weeks, DZ preconditioned DM-EPCs transplanted group showed improved contractility of diabetic heart as compared to untreated DM-EPCs group. There was decrease in collagen content estimated by Masson''s trichrome and Picrosirius red staining in DZ DM-EPCs transplanted group as compared to untreated DM-EPCs transplanted group. Furthermore, reduced cells injury were observed in DZ DM-EPCs group as evidenced by decreased expression of caspase-3 and increased expression of prosurvival genes Bcl2, VEGF and bFGF by semi-quantitative real time PCR. X In conclusion, the present study demonstrated that DZ preconditioning enhanced DM-EPCs survival under oxidative and hyperglycemic stresses and their ability to treat DCM.