مقدراں دی کھیڈ
ناہید اک وڈے سرکاری افسر دی اکلی اولاد سی۔ ایس لئی اوس دی پرورش بہت ای چنگے ڈھنگ نال ہوئی اوس نوں ماں تے پیو دا رجھواں پیار وی ملیا۔ جدوں اوس نے بی ایس سی دا امتحان دتا تاں اوس دے گھر کئی رشتے آنا شروع ہوگئے۔ اک دن ناہید دی ماں دی سہیلی اوہناں دے گھر آئی تے مینوں اپنے پتر اعجاز لئی منگ لیا۔ حالے اوہدا نتیجہ وی نئیں سی آیا کہ اوہ ناہید اعجاز بن گئی۔
اعجاز اک چنگا، سوہنا تے کماؤ پتر سی۔ گھر دے سارے جی ناہید دا خیال رکھدے سن۔ کیوں جے اعجاز اک سرکاری ملازم سی ایس لئی اک سال لنگھن دا پتہ وی نہ لگا۔ ویاہ توں اک سال بعد میں اک پتر دی دی ماں بن گئی۔ پتر دا ناں آصف رکھیا گیا۔ آصف دے جمن تے سارے گھر والے خاص کر سس سوہرا تے جیٹھانی بہت خوش سن۔ اوس دی وڈی وجہ ایہہ سی کہ میرے جیٹھ دی کوئی اولاد نئیں سی۔ سارے ای آصف نال انتہا دا پیار کردے سن۔
ایہناں دناں وچ بہت کم ہوون پاروں اعجاز بوہتا مصروف ہوگیا، کدے رات نوں چھیتی گھر...
"An analysis of the allegations of extremism and terrorism against religious institutions (Madrasas)". The priceless services done by the religious scholars for the preservation and uplift of religious and Islamic values in the subcontinent are indelible and unforgettable chapter of history. They geared up progress of religious institutions and the tilt of people towards them of the increasingly charming trend. The Heathen world is afraid of the emerging strongholds of Islam. The repercussions of this trend on society are becoming more and more prominent with the march of time. They are striving for the preservation and identity of the Islamic characteristics. After 9/11 incidents, the west is unable to understand how to detach the religious institutions from the embedded Islamic social integrity. The western media and foreign funded rulers have been endeavoring hard to defame religious institutions through there venomous propaganda against them. All this is visible to everyone. There is no parallel of the religious institutions educational boards (Wafaqs) in and outside the country even no such example is present in the whole Islamic world as well as in the subcontinent. Besides other baseless allegations, religious institutions are branded as terrorists and extremists. The west and America are much worried about the Islamic educational institutions and the Holy war (Jihad). The article encompasses the opinions of the regious as well as secular apostles. In a nutshell, all the allegations of extremism and terrorism are not only baseless but just a propaganda.
EXPANSINs are the intrinsic proteins in a plant cell and are involved in disentangling the cellulosic microfibrils of the cell wall. The mechanism of EXPANSIN action is generally concerned with cellular expansion. This family of proteins has also been documented to have tissue specific members. Multiple variants of this protein have been identified in specific tissues, which are temporally regulated and functionally specific. The fiber specific EXPANSINs play a key role in the development of cotton fibers. Various isoforms of EXPANSINs were isolated by screening the cDNA libraries constructed at different developmental stages of cotton (Gossypium hirsutum) and Calotropis procera fibers. Nucleotide sequence analysis of the screened clones helped to identify two major variants in cotton (GhEXPA8 and GhEXPA15) and four EXPANSIN isoforms in fast elongating C. procera fibers. The C. procera CpEXPA3 was selected for further analysis on the basis of its close relatedness with cotton fiber EXPANSINs. The comparative analysis of these EXPANSINs with existing database of the gene family revealed that they belong to the third clade of EXPANSIN A family having two characteristic domains. Eight conserved cysteine residues were found in the N-terminal of the deduced amino acid sequence of GhEXPA8, GhEXPA15 and CpEXPA3, while one was in the signal peptide region. Five tryptophan residues were conserved in C-terminal region of these EXPANSINs. The Amino Acid sequences of GhEXPA8 and GhEXPA15 have 98 % identity, while they have 69.8 % and 69.6 % identity with CpEXPA3 respectively. The presence of the signal anchor sites, the hydrophobic regions and the transmembrane regions at the N-terminus suggested that these proteins are targeted to the cellulosic microfibrils through the secretory pathway. The expression of these variants in different tissues was quantified by real time PCR. The transcripts of GhEXPA8 and GhEXPA15 were observed only in fibers, while CpEXPA3 was found to be transcribed nonspecifically in all tissues of the respective plant. The different transcription patterns of GhEXPA8 and GhEXPA15 at various stages of fiber development indicated that they are functionally different genes. The real time PCR analysis indicated the presence of EXPANSIN variants in developing cotton fibers from 0-15 DPA. The RT-PCR demonstrated that transcripts of LTP3 gene could be detected in developing fibers from 0-20 DPA. A plant expression vector was constructed by fusing LTP3 promoter with a reporter gene (GUS with intron) for in 20Generated by Foxit PDF Creator © Foxit Software http://www.foxitsoftware.com For evaluation only. Masooma Thesis vitro expression assay of the promoter strength and specificity in comparison with 2X35S. Transient expression studies on cultured cotton ovules, sepals, petals and stem revealed that the LTP3 promoter activity was confined only to the trichomes. The GUS gene in the expression cassettes was replaced with GhEXPA8, GhEXPA15 and CpEXPA3 generating six EXPANSIN genes constructs under the two promoters (2X35S and LTP3) with an aim to prolong the EXPANSIN gene expression in developing fibers. The construction of the expression cassettes was verified by DNA sequencing and the constructs were handed over to cotton transformation group at NIBGE for their utilization in fiber modification program.