دھب میںڈھابہ ہوٹل
شرم الشیخ سے پچاس میل کی مسافت پر دھب نامی قصبہ پر ہمار ی گاڑی چائے کے ایک ریستوران پر رکی ۔یہاں پاکستانی ٹرک ڈرائیور ہوٹل جیسا ماحول تھا ۔گاڑی میں موجود مصری خواتین نے اپنے بڑے بڑے پرس کھولے تو مجھے حیرت ہوئی کہ ان پرسوں میں توشۂ خوراک یعنی پنیر ،ڈبل روٹی ،بسکٹ ،سیب ،مالٹے ،کھجور اور پانی کی بوتل تک سارا انتظام مکمل تھا ۔مجھے اس انتظام و انصرام سے زیادہ خواتین کی فیاضی نے بہت متاثر کیا ۔ سب نے ایک دوسرے کے ساتھ اپنے توشے شریک کیے ،باوجود اس کے کہ سب کے پاس یہی چیزیں خود بھی موجود تھیں ۔سوڈان کے دکتوریحیٰ اور میرے پاس ان اشیا ء میں سے کوئی چیز نہ تھی ۔اس لیے سب نے ہمیں نوازنا شروع کر دیا اور آخر میں ہم دونوں کے پاس خوراک کا ذخیرہ ان سے بھی زیادہ ہو گیا ۔میں نے دکتورہ بسنت کو کہا کہ پاکستانی خواتین کے پرس اس سے بھی بڑے ہوتے ہیں مگر وہ پائوڈر ،کریم سے لیس ہوتے ہیں ۔ اس نے ہنس کر کہا وہ چیزیں ہمارے پاس بھی ہوتی ہیں مگر الگ پرس میں ۔ہوٹل میں مختلف قسم کے بسکٹ کے ساتھ نفیس پیکنگ میں ایک چیز بند تھی ،میں نے اس کے بارے میں دکتورہ شائمہ سے پوچھا کہ یہ کیا ہے ؟انھوں نے کہا ’’خشک روٹی ‘‘میں پوچھا یہ بھی یہاں فروخت ہوتی ہے ۔اس نے بڑے اعتماد سے کہا کہ ہاں بالکل اور لوگ یہاں اس کو بڑے شوق سے کھاتے ہیں۔ ہوٹل کے عقب میں بیری کے دو تین درخت تھے جن پر پھل پک چکے تھے ،میں نے توڑ کر کھائے تو محسوس ہوا کہ تپتے صحرا نے ان کو پکانے اوررات کی چاندنی نے ان کو میٹھا بنانے میں کوئی کسر نہیں...
This paper is formulated under the conceptual framework of Gayatri spivak’s theory of Can Subaltern Speak? And explores the potential permeability of visual resources as a form of discourse through which subalterns found opportunity to counter media hegemony and make their voices heard. In order to analyze the selected graphic novel Munnu: A Boy From Kashmir (2015) the present qualitative study applies Grunter Kress and Theo Van Leeuwen’s model of visual design (2010) that connects the representational meaning to the interactive one. The analyst reflected upon how the interplay of visuals images and words together displayed the theme of Kashmir’s subjugation as well as how this genre has proved supportive to author to counter the said hegemony. Hence, the study found consent, political domination and media control as the broad elements that can be seen in the novel and also the study concludes that counter hegemony is possible through such literary genres, as the novel’s narrator has communicated those aspects of hegemonic situation in Kashmir to a large audience through literary discourse of graphic novel genre that are chiefly absent from mainstream media’s treatments of the Kashmir’s conflict. ______
In this thesis, the medical condition of sepsis is considered at molecular level (signalling pathways) using computational systems-level approaches in order to get insights into the mechanism of disease progression. Sepsis is the pathological condition provoked due to the presence of bacterial endotoxin in the bloodstream. Subsequently Toll like receptors (TLR)4 and JAK/STAT signalling pathways attempt to reduce the pathogen burden by inducing pro- and anti-inflammatory innate immune responses. However, in some instances, an overwhelmed immune system could not properly regulate the balance between infection and inflammation that may ultimately lead to organ damage and consequently to death. In recent years, there has been an increasing amount of literature on the pattern of pro- and anti-inflammatory response elicited during sepsis, though; there has been a little agreement on the roles of pro- and antiinflammatory cytokines (AiCyts) in sepsis. This study mainly aims to address the controversy behind roles of pro- and anti-inflammatory cytokines in sepsis by modelling the signalling pathway of TLR4 and one of the connected signalling cascades of JAK/STAT using qualitative modelling approach introduced by René Thomas’. The possible system dynamics of TLR4-JAK/STAT signalling pathways are produced for two medical conditions i.e. non-sepsis (type of infections that generally do not cause sepsis) and sepsis along with perturbations in these two cases. As a result, recurrent induction and inactivation of pro-inflammatory cytokines is found as the basic feature associated with sepsis. Besides AiCyts, IFN-β and SOCS-1 are found to mediate down-regulation of pro-inflammatory cytokines at different stages of signalling which cause variation of pro-inflammatory cytokines levels. It is observed that intervention in IFN-β mediated down-regulation of pro-inflammatory cytokines at earlier stages of system dynamics, while intervening the SOCS-1 mediated down-regulation of proinflammatory cytokines at later stages ensures hyper-inflammatory condition. On the other hand, interventions in TLR4, NFκB (transcription factor involved in the TLR4 1 Abstract signalling pathway) and JAK/STAT signalling are good choices for supporting the anti-inflammatory immune responses. Thus only possible protein-protein interactions involved in the initial downstream interactions of TLR4 signalling are studied in order to predict a more appropriate target in these interactions. Previous studies indicated that MyD88 adaptor-like protein (MAL) is an endogenous adaptor protein recognized as an important protein involved in the induction of TLR4 mediated downstream signalling pathway. Moreover, it has also been demonstrated that BTK and PKCδ phosphorylate MAL (positions Tyr86 and Tyr106) which ultimately activates MAL. Thus the modelling of PKCδ and protein-protein interactions of both BTK and PKCδ with MAL is performed in order to explore their competitive interaction. Molecular docking and physicochemical analysis reveals that PKCδ may phosphorylate only Tyr106 of MAL, while BTK may phosphorylate MAL at both positions. Interestingly, the charge and hydrophobicity at interfaces of PKCδ and BTK are found different in nature yet well-compatible with the individual positions of Tyr86 and Tyr106 of MAL. The most prominent findings emerged from this analysis is that position Tyr86 of MAL may explicitly be phosphorylated by BTK, while position Tyr106 of MAL may be phosphorylated by the competing interest of both PKCδ and BTK. In conclusion, this thesis will enhance our understanding about the signalling and protein-protein interactions involved in sepsis which will contribute to the development of drugs and vaccines against the medical condition of sepsis.