اُمّیدبنو،تعمیر کرو سب مل کر پاکستان کی
نحمدہ ونصلی علی رسولہ الکریم امّا بعد فاعوذ بااللہ من الشیطن الرجیم
بسم اللہ الرحمن الرحیم
معزز اسا تذہ کرام اور میرے طالب علم ساتھیو!
آج مجھے جس موضوع پر لب کشائی کا موقع فراہم کیا گیا ہے وہ ہے:’’اُمّیدبنو،تعمیر کرو سب مل کر پاکستان کی‘‘
صدرِذی وقار!
امید مایوسی کو جڑ سے کاٹ کر رکھ دیتی ہے۔ قنوطیّت ا ور نا امیدی کی فضاء میں نشوونما پانے والا شجرکبھی بارآور ثابت نہیں ہوتا، امید ا یک نوید ِجاں فزا ہے، اُمید کی کرن ظلمت کدہ جہاں میں نور ثابت ہوتی ہے، امید کے سہارے چلنے والاشخص کبھی نہ کبھی در منزل پرضرور دستک دیتا ہے۔
صدرِمحفل!
پاکستان ہمارا ملک ہے، پاکستان ہمارا وطن ہے، پاکستان ہماری پیاری سرزمین ہے، پاکستان ہماری جنت ہے، پاکستان کا ہر ذرّہ ہمیں جان سے بھی زیادہ پیارا ہے، پاکستان کے گل وگلستان پاکستان کے صحرا اور ریگستان ، پاکستان کے کھیت اور کھلیان ، پاکستان کے مزدور اور اور دہقان یہ سب ہمارے ہیں۔
صدرِذی وقار!
اس کی جامعات ہمیں زیورِ تعلیم سے مزیّن کرتی ہیں، اس کے محراب وممبرسے ہمارے لیے وعظ و نصیحت کی صدائیں بلند ہوتی ہیں ، اس کے کھیت وکھلیان ہمارے لئے رزق وافر کا انتظام و انصرام کرتے ہیں، اس کی عدالتیں ہمارے لیے انصاف کا بندوبست کرتی ہیں اس کے گلستان و چمنستان ہمارے لیے نکہت و باد بہاری کا سامان بہم پہنچاتے ہیں۔
جنابِ صدر!
تاجر ایماندار ہو گا تو تجارت معیاری ہوگی، منصف مجسمہ خلوص ہوگا تو عدالت کی کرسی اقربا پروری اور رشوت ستانی کی گرد سے صاف ہوگی ، واعظ وخطیب جب صاحب علم و عمل ہوگا تو محراب وممبر سے بلند ہونے والی آواز یں پُر تاثیر ہوںگی ، باغبان کی نیت ٹھیک ہوگی تو گلشنِ...
Education has been considered of primary importance in human life. Religion and education are interrelated. A civilized society requires educational revolution in behavior of human beings. Education creates awareness about the human goals and real aim of life. This activity makes it possible to train the people in the right direction. Better education coupled with proper training makes a human being well-mannered and enables him to use his hidden qualities for the benefit of mankind, thus making him a beneficial not only for himself but also for the society. The preaching of religion depends upon the education and training. Our holy Prophet ﷺ was an ideal educationist not only of his time but also for the future generations. The Holy Prophet ﷺ being a great educationist and reformer initiated the University of Suffa in Medina when the Islamic society was in its infancy. Prophet ﷺ described the conditions, pattern and qualities of a teacher. A good teacher must be interpreted as good practitioner as well. The article deals with the moral qualities of a teacher and educationist in the light of teachings of the Prophet Muhammad ﷺ. The discussion has made clear the aim of Islamic education which is not limited within the domain of worship or prayers. Islam is a complete code of life which emphasizes the training of human being through education.
This study was designed to study various classes of synthetic organic compounds for their antioxidant activities in molecular and cellular models of oxidative stress. Etiology of numerous types of cancers has been linked with oxidative stress, especially, metastatic melanoma is known as a reactive oxygen species (ROS) driven tumor.In this study, five metastatic melanoma cell lines (A375, WM266-4, SK-Mel-28, BLM, and MV3), and two non-metastatic melanoma cell lines (WM-115 and Mel-HO) were used to detect ROS association with neoplastic transformation relative to normal cell lines wound healing fibroblast (CPD95) and normal human melanocytes (NHEM). However, corelation of ROS production with metastatic propensity of malignant melanoma cell lines could be found. Out of over 1,000 compounds evaluated during this study, 250 synthetic organic compounds showed free radical scavenging activity in the DPPH radical scavenging assay. From them 34 synthetic organic compounds (17 free radical scavengers with low to high free radical scavenging activity, along with 17 non-radical scavenger homologues) were selected for further biomolecular studies. These compounds were evaluated for their cytotoxicity, using the MTT assay. Eight different synthetic organic compounds of four different classes, pyrimidine derivatives 1 and 4, biscoumarin derivatives 10 and 11, thiazole derivatives 6 and 8, and aryl Schiff’s bases 13 and 15, were identified as potential anti-melanoma agents due to their cytotoxicity against both metastatic (A375, and SK-Mel-28), and non-metastatic cell lines (WM-115) cell lines. The identified potential anti-melanoma agents were further evaluated against normal cells lines wound healing fibroblast (CPD95) and normal human melanocytes (NHEM), and all of them were found to be non-cytotoxic against (NHEM). However, one of the bis-coumarin derivatives 11, one thiazole derivative 8, and both of the pyrimidine, derivatives 1 and 4 were found non-cytotoxic to the fibroblast cells. These compounds were further tested for their anti-migration properties. Both derivatives of the class thiazole, aryl Schiff’s bases, and bis-coumarins were found to behave as antimigration agents, wherase none of them affected intracellular ROS production of A375 cells. DNA synthesis inhibition activity of these compounds was also evaluated using the BrdU incorporation assay; all compounds were found to inhibit DNA synthesis in the metastatic melanoma (A375 cell line) cancer cells. A non-radical scavenger, bis-coumarin derivative 11 with anti-migration and anti-proliferation activities, was tested on 43 different kinases phosphorylation sites, and 2 related proteins (heat shock and tumor supressor) at two different time ponits using proteome profiler human phospho-kinase array assay. Compound 11 was found to target 40 different phosphorylation sites of 25 different kinases. The mammalian target of rapamycin (mTOR) pathway kinases (mTOR and PRS40), as well as tumor suppressor proteins (Tp53 and Chk-2 kinase) that are involved in melanoma cell growth and tumor suppression, respectively, were found to be the significant targets of compound 11. Compound 11 was also found to inhibit melanoma cell survival, and growth at gene level via inhibiting phosphorylation of STATs family of transcription factors through the JAK- STAT pathway. Compound 11 was also found to inhibit the melanoma cell survival and proliferation by inhibiting phosphorylation of ERK1/2, MSK1/2, and CREB via EGF and PDGF receptor-dependent, and intracellular redox-independent signaling pathways. Members of Src family of kinases implicated in melanoma migration/metastasis were found to be additional targets of this compound. Compound 11 was effective in inhibiting metastatic melanoma proliferation, and cell migration. For the first time it was identified as a novel anti-melanoma agent of the bis-coumarin class that could suppress the melanoma cell growth along with its metastatic propensity without affecting normal cells. Based on these findings, the lead compound 3,3''((3,5Dichlorophenyl) methylene) bis (4-hydroxy-2-H-chromen-2-one) (11) was identified as a potential anti-melanoma and anti-metastatic drug candidate.