مایوسی گناہ ہے
اللہ تعالیٰ نے انسان کو اشرف المخلوقات بنایا اور پھر اس کے سر پر عظمت کا تاج سجایا۔ اس کو دیگر مخلوقات پر فوقیت دی، اس کی عظمت کا راز اس کی عقل سلیم میں رکھا کیونکہ دیگر مخلوقات میں شرف و بزرگی کا عنقاء عدم عقل و خرد ہے، اور پھر اُس کوصحیح اور غلط کی پہچان نصیب فرمائی۔ اور اُس کی رفعت کے حصول کے لیے کوشش کومحمودگردانا۔
ارشاد ِباری تعالیٰ ہے کہ ’’انسان کے لیے وہی کچھ ہے جس کے لیے وہ کوشش کرے۔‘‘
اگر ہاتھ پر ہاتھ دھرے بیٹھار ہے اور تگ و دو اور شبانہ روز کوشش سے دست کش ہو جائے مایوسی کے ظلمت کدہ کا مکیں بن جائے ، نا امیدی کے عفریت کے جبڑوں میں پھنس جائے تو پھر بلیّات و مصائب کے مہیّب سائے تو اُس کے آنگن میں آ سکتے ہیں ، خوش بختی اور خوش نصیبی کے آفتاب کی کرنوں سے اس کا گھر محروم رہے گا۔
مایوسی و یسے گناہ ہے۔ نا امیدی کے سائے کے نیچے پروان چڑھنے والا پودا کبھی شجر سایہ دار نہیں بن سکتا۔ نا امیدی کے گلستان میں کھلنے والے گلہائے رنگا رنگ خوشبو کی راحت افزاء مہک سے عاری ہوتے ہیں ، مایوسی کے خار ہائے نوک دار پر پاپیادہ شخص آبلہ پائی کا شکار ہوسکتا ہے ہریرو پرنیاں اور مخمل کا احساس اُس سے کوسوں دور ہوتا ہے۔
قرآنِ پاک میں ارشادِ باری تعالیٰ ہے کہ ’’ لا تقنطو من رحمۃ اللہ‘‘اللہ تعالیٰ کی رحمت سے نا امید مت ہوں ۔ بحثیت مسلمان تو نا امیدی ویسے بھی گناہ اور حرام ہے۔ انسان جب امید سے اپنے دامن کو پر رکھتا ہے تو مسرتیں اور راحتیں اس کے دروازے پر دستک دیتی رہتی ہیں۔ اور امید ہی کی کرن اس کو حیات نوبخشتی ہے۔
کسان...
Muslims are a unique nation in the world who have shown unparalleled activism in preserving their sayings and relics. Caution was in view from day one. The simple precautionary measures taken in the early days later took the form of theology. In the beginning, the principles and rules of this knowledge did not exist in a disciplined form, although the scholars of Muḥaddithin adhered to the basic principles of this knowledge from the very beginning. However, references to these principles were to be found in the books of the pious. In the 4th Hijri, the scholars of Muhaddithin compiled the previous scattered work on the art of the principle of Ḥadith. Now these comprehensive definitions and terms are before us in a systematic and disciplined form in the books of ‘Usūl e Ḥadīth. In this short essay, the definition and necessity and importance of Hadith and ‘Usūl e Ḥadīth have been highlighted and also an introductory review of books written on ‘Usūl e Ḥadīth th and their authors and authors has been presented. Which indicates this. How strict were the rules and regulations of the Muslim Ummah for examining the authenticity and text of the Holy Hadith and how did they separate the authentic, weak and subject matter of the hadiths and write their separate books? And how hard they have worked to promote art.
Enzyme immobilization utilizing distinctive methodologies and different supports appears to be the most effective possibility for developing industrially compatible enzymes. Immobilization has been discovered as a capable device for enhancing all catalyst properties, specific activity, thermo stability and decreasing inhibition. Laccase with improved catalytic features is a potentially attractive biocatalyst with many possible applications including bioremediation, biomass delignification for (590706) 4.58 Visual comparison of decolorization of dyes with free and immobilized laccase with chitosan bead (a) Sandal-fix Red C4BLN (b) Sandal-fix Golden yellow CRL (300410) 138 4.59 Visual comparison of dye degradation (A) control (B) degradation with free Laccase (C) degradation with laccase immobilized with PVA beads (D) degradation with laccase immobilized with Ca-Alginate beads 140 4.60 Repetitive use of immobilizedlaccase for dye decolorization 142 4.61 Storage stability of free and immobilized Laccase at 16oC 144 ethanol production, chemical synthesis, bio pulping and biosensor development, denim stone washing, cosmetics, dyes degradation, textile finishing and wine stabilization. In this study, four copper containing extracellular laccase was purified from culture filtrate of an indigenous WRF strain Trametes Versicolor IBL-04, grown in solid state fermentation (SSF) under pre optimized condition using corncobs as a substrate. The enzyme was purified (1.00-4.03 fold) to apparent electrophoretic homogeneity through ammonium sulphate precipitation, dialysis, DEAE-cellulose ion exchange and Sephadex G-100 gel exclusion chromatography. The purified enzyme elucidated a monomeric single band on Native and SDS polyacrylamide gel electrophoresis (SDS-PAGE) with an apparent molecular weight of 66 kDa. With an aim to improve its functionalities, the purified laccase was effectively immobilized on nine supporting matrices using diversified immobilization techniques and the relative activity at optimum temperature and pH and thermal stability of free and immobilized laccase were studied for comparative analysis. Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) were performed to characterize enzyme immobilization. Immobilized laccase was successfully used for the decolorization of six different synthetic (textile) dyes with more than 98% removal rate. The resulted modified laccase enzyme was reusable for up to many cycles in dye color removal and also check the storage stability and thermostability of immobilized laccase. All immobilized parameters showed that immobilized laccase is better for industrial use in comparison to free laccase. Finally results of this study suggested that immobilized laccase catalytic system can be efficiently exploited for dye degradation due to its sustainability, durability and reusability over its soluble counterpart. Keywords: Trametes Versicolor IBL-04, Laccase, purification, characterization, immobilization, dye decolorization