پتھر کی دنیا
آج بھی دنیا پتھّر کی ہے
نہیں ہے بدلی ریت ہماری
کوئی بھی چیز نہیں ہے بدلی
پیار نہیں سمجھے گا کوئی
آ اِس پتھّر کی دنیا میں
میں بھی پتھّر ہو جاتا ہوں
تم بھی پتھّر کے ہو جائو
آ اِس پتھّر کی دنیا میں
ہم بھی پتھّر ہو جاتے ہیں
اب بھی دنیا پتھر کی ہے
اِس کی ہر شے پتھر کی ہے
چاند بھی پتھر تارے پتھر
چشمے پتھر دھارے پتھر
ریت رواج ہمارے پتھر
آ اِس پتھّر کی دنیا میں
ہم بھی پتھّر ہو جاتے ہیں
آ اِس پتھّر کی دنیا میں
ہم دونوں پتھّر ہو جائیں
This study aims to calculate the allowance for losses by applying Gaussian Process regression to estimate future claims. Modeling is done on motor vehicle insurance data. The data used in this study are historical data on PT XYZ's motor vehicle insurance business line during 2017 and 2019 (January 2017 to December 2019). Data analysis will be carried out on the 2017 - 2019 data to obtain an estimate of the claim reserves in the following year, namely 2018 - 2020. This study uses the Chain Ladder method which is the most popular loss reserving method in theory and practice. The estimation results show that the Gaussian Process Regression method is very flexible and can be applied without much adjustment. These results were also compared with the Chain Ladder method. Estimated claim reserves for PT XYZ's motor vehicle business line using the chain-ladder method, the company must provide funds for 2017 of 8,997,979,222 IDR in 2018 16,194,503,605 IDR in 2019 amounting to Rp. 1,719,764,520 for backup. Meanwhile, by using the Bayessian Gaussian Process method, the company must provide funds for 2017 of 9,060,965,077 IDR in 2018 amounting to 16,307,865,130 IDR, and in 2019 1,731,802,871 IDR for backup. The more conservative Bayessian Gaussian Process method. Motor vehicle insurance data has a short development time (claims occur) so that it is included in the short-tail type of business.
Laboratory experiments were conducted to screen plants i.e. Celosia argentea, Celosia cristata, Mirabilis jalapa, Mangifera indica, Moringa oleifera, Ziziphus jujuba, Ziziphus mauritiana, Delonix regia, Morus alba, Albizia procera and Albizia lebbek, for their allelopathic potency on Lepidium sativum. Maximum inhibition (97%) to root elongation was observed at 10% concentration of Celosia argentea extract; however, Mangifera indica at 10% concentration totally inhibited the germination of seeds. On the basis of results of present studies and literature survey, Celosia argentea was selected for further study. Different concentrations of Celosia argentea parts i.e. roots, stem, leaves and flowers were compared for their; (a) allelopathic potential (b) total phenolic contents and (c) individual phenolic compounds through Mass Spectrometry (MS). Based on literature survey and present studies, leaves of Celosia argentea were selected for isolation, purification and identification of herbicidal compounds using bioassay guided isolation method. Solvent extraction method using different solvents and High Performance Liquid Chromatographic (HPLC) method using different solvent systems were optimized.Finally, two types of extraction methods i.e. aqueous and methanolic extractions were used. Aqueous and methanol extracts were partitioned with n-hexane, then with ethyl acetate and the remaining extracts were divided into two parts. One part of each extract was subjected to acid hydrolysis to release the phenolic compounds from sugar moieties and then extracted with ethyl acetate, while second part was lyophilized. All the organic solvents were evaporated and residues were dissolved in methanol to prepare 4000 ppm solutions and were used for bioassay study against Lepidium sativum. The highest inhibition of seed germination (100%) was caused by acid hydrolyzed and ethyl acetate fractions followed by lyophilized fraction. Fractions with inhibitory potency i.e. ethyl acetate, acid hydrolyzed and lyophilized fractions were used for analysis through Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC) and Liquid Chromatography Mass Spectrometry (LCMS). More than 60 compounds including quercetin, quercetin 3-O-glucoside, quercetin pentose glucuronide, gallic acid, gallic acid 4-O-glucoside, m-hydroxybenzaldehyde, pholoroglucinol, phloroglucinol glucoside, m-coumaric acid, catechol etc. were identified as allelopathic agents. Most inhibitory fractions i.e. ethyl acetate and acid hydrolyzed fractions of methanolic extract were further divided into five fractions through Preparative Thin Layer Chromatography. Different concentrations of five fractions that were obtained from this separation were subjected to bioassay study against Lepidium sativum. The active fractions i.e. F2 and F5 were further separated on Preparative Thin Layer Chromatography in order to purify individual compounds. Active compounds were then analyzed with LCMS and Nuclear Magnetic Resonance (NMR). To our knowledge compounds such as 3,5-dihydroxybenzaldehyde, p-coumaric acidwere first time identified as herbicidal compounds in Celosia argentea. In addition to this work, Mirabilis jalapa, Mangifera indica, Delonix regia, Morus alba and Moringa oleifera leaves were also subjected to bioassay guided isolation method and active fractions were analyzed for allelopathic compounds using HPLC and MS.