پاکستان میں بچوں پر جنسی تشدد اور فقہ السیرہ کی روشنی میں تدارک

Numerous crimes are being committed in present era. One of the most heinous crime amongst them is child abuse. Incidents of children who have been murdered after sexual abuse are rife in our nominal Islamic society. Moreover, videos and photos are also made during child abuse. Then these videos are posted on the social media. Both Muslim and non-Muslim communities are suffering from this brutal act i.e child abuse. In Pakistan, the reasons of increase in crime rates include psychological disorders, urge to fulfill one’s sexual desires, poverty, unemployment and detachment from religion. Such kind of abominable acts are becoming common in Pakistan day by day. According to Islamic jurisprudence rape is a punishable crime. Holy Prophet Muhammad (SAW) himself has punished the perpetrators for such acts of crime. Once in the era of Holy Prophet (SAW), a Jew crushed the head of a girl by placing it in between two stones. When Prophet (SAW) was informed about the incident, He (SAW) called upon the Jew who confessed his crime. Then Prophet (SAW) asked his companions to punish him by crushing his head with stones. It can be deduced from this narration that the culprits involved in such loathsome crimes should be punished publicly in order to wipe out this devilish act of crime from the society. The purpose of writing this research paper is to describe the reasons for the increase in rate of sexual abuse of children and its eradication in the light of Fiqh ul Seerah. This research paper followed descriptive and analytical methods

Mutagenesis of Indigenous Streptococcus Equisimilis Isolates for Enhanced Production of Streptokinase

Acute myocardial infarction and ischemic stroke are caused by thrombosis or the obstruction of blood vessels with clots and this is the leading causes of death. The single handling accessible is the use of thrombolytic agent to liquefy the blood lump. Hyper production of streptokinase was carried out in this research work. Beta hemolytic Streptococcus equisimilis was isolated from indigenous sources and then mutagenesis of this isolate was carried out by means of chemicals as well as radiations. To seek the optimum activity, different kinetic and thermodynamic parameters like pH, temperature, Km, Vmax, molecular weight, melting temperature, half-life, enthalpy and entropy etc. were applied on the purified enzyme. UV irradiated strain resulted in 335 U/mL activity with 1116.66 U/mg specific activity, 0.30 mg/mL protein, 41.92 fold purification and 69.79% recovery whereas Sodium azide derived mutant resulted in 400 U/mL activity with 2000 U/mg specific activity, 0.20 mg/mL protein, 71.94 fold purification and 64.51% recovery of the finally purified enzyme. Gamma irradiated strain exhibited 300 U/mL activity with 1428.57 U/mg specific activity, 0.21 mg/mL protein, 59.52 fold purification and 75.94% recovery whereas ethidium bromide derived mutant showed 365 U/mL activity with 1659.09 U/mg specific activity, 0.22 mg/mL protein, 66.52 fold purification and 85.08% recovery. Optimum pH and temperature of the finally purified enzyme was 7 and 45oC. Enthalpy of denaturation (ΔH*) of streptokinase at 450C was 43.67 kJ/mole. The Energy of thermal denaturation ΔG* was 101.14 kJ/mole and entropy of inactivation ΔS* was -197.32 kJ/mole at 45oC. The negative value of ΔS* indicated that streptokinase was thermodynamically stable. Km and Vmax values of streptokinase were 26.31 mM and 50 MS-1. Streptokinase produced from sodium azide derived mutant exhibited activity within the pH range of 6 to 8 while it presented its best performance at pH 7. Thermal stability between 45oC to 80oC was shown by the streptokinase along with half-life of 244 minutes while less stability was shown at 80oC along with 45 minutes of half-life and 40.41 kJ/mole as enthalpy of denaturation (ΔH*).