حرص و ہوس کا زہر نکل جائے دل سے کاش !
ہو جائے ختم چٹکی میں فکر ۔ غم۔ معاش
کندھوں سے اپنے بوجھ اتار اس کی یاد کا
کب تک اٹھا کے پھرتے رہو گے یہ مردہ لاش
اے عشق ! تیرے حوصلے کی داد شرط ہے
پہلو میں حسن تھا مگر آئے نہ دی خراش
تنہائی کا شکار تھا وہ شخص اس قدر
میلے کی بھیڑ میں جسے اپنی رہی تلاش
پھر یوں ہوا کہ نیند ہی آنکھوں سے اڑ گئی
یہ کس نے کر دیے ہیں مرے خواب پاش پاش
زیر ۔ زمین کوئی رگڑتا ہے ایڑیاں
پیدا بلا جواز نہیں ہوتا ارتعاش
کانوں میں تیل ڈال کے سویا نظام عدل
پیدا ہوئے ہیں چوک چوراہے میں بد معاش
شاہد ! پرائے بت پہ نہیں لازم انحصار
بہتر ہے اپنے ہاتھ سے تو اپنا بت تراش
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The history of the business and trade is as old as the history of humanity, itself. Different cultures and regions have their own business ethics throughout the times past. With the rise of globalization, the various sets of business ethics are getting common values and forms. While Islam, being a universal religion, provides guidelines for every aspect of life including trade, commerce and business. Islamic business ethics derive from divine revelation and Sunnah of the Prophet (SAW). Business ethics denotes the behavior of trading entity supposed to follow while interacting with society. The Islamic business ethics take care of the all stake holders from production to consumption including employees, trade agreements, quality standards, environment and social responsibilities. The current paper is aiming to elaborate the business ethics in the light of instructions draw from the Seerah of the Prophet (SAW) in order to provide an insight for humanity to follow them, practically
Cytoplasmic dynein is a multi-subunit complex that transports cargos along microtubules towards their minus end. Dyneins are very essential for vesicular transport, maintenance of golgi apparatus, spindle formation and cellular homeostasis. They are tightly regulated by the interactions of individual dynein subunits and accessory proteins that are implicated in a wide range of dynein-driven transport events. Malfunctioning of dynein light chains has been associated with a variety of human diseases. Current study is comprised of four projects with the major emphasis on characterization of differentially expressed genes in wild-type verses Tcte3-3 disrupted mice, involved in male germ cell apoptosis. In the first study, basic aim was to explore and evaluate mouse Tcte3 paralogs through Bioinformatics and experimental approaches. Significant changes were observed among Tcte3 paralogs using multiple sequence alignment and restrictions maps analysis. Furthermore, we monitored the expression of Tcte3 paralogs in brain and testis tissues of wild-type and Tcte3-3 disrupted mice. Our findings suggested that observed Tcte3 paralogs exhibited tissue specific expression. Next, we employed two-dimensional gel electrophoresis and quantitative reverse transcriptase PCR approaches and performed detailed in-silico analysis of microarray data extracted from NCBI gene ontology omnibus (GEO) to isolate candidate hits influenced by Tcte3-3 disruption. Our findings elucidated several co-expressed partners of Tcte3 including Anxa5 and Pebp1, whose functional coherence may help in better understanding of apoptotic induction. In another study, to evaluate the gene expression profile of testis and brain tissues, Tcte3-3 knockout mice were characterized by differential gene expression profiling technique using cDNA microarray technology (Illumina Beadchip microarray). Isolated data were analyzed and converted into biologically meaningful form by integrative transcriptomics and proteomics approaches. Furthermore, based on the differential gene expression; gene ontology analysis, pathway analysis and protein-protein interaction mapping analyses were performed. Microarray results were further validated by qRT-PCR, immunoblotting, molecular docking and dynamics simulation analyses. Overall, differential microarray study of Tcte3-3 disrupted mice coupled with detailed Bioinformatics analyses provided an updated information and overall insight into Tcte3 mediated gene regulation thus offering useful clues to counteract or prevent its detrimental consequences. Our results highlighted several differentially expressed genes overrepresented in Gene Ontology categories. Furthermore, molecular interaction and reaction pathways involved in male germ cell apoptosis, cell cycle, lipid metabolism and molecular movement were displayed. As Tcte3 is essential for spermatogenesis, our study revealed valuable resources for the exploration of Tcte3-linked functions in male reproduction. Finally, we analyzed functional conservation of three dynein light chains by exploring their molecular structures to find common interaction networks. We proposed involvement of dynein light chains in apoptotic process via their consistent interactions with common binding partners. Based on this notion, we monitored that Tcte3 and Tctex1 exhibited a similar binding pattern against the previously reported interaction partners of DYNLL1. Taken together, our in-silico and supporting experimental data revealed several novel interaction partners of Tcte3, Tctex1 and Dynll1 with overlapping roles in intrinsic apoptosis pathway. Together, with the exploitation of Tcte3 mediated functions, this study may serve as a valuable resource in understanding complex mechanism of apoptosis. Collectively, our study may contribute towards better understanding of tissue-specific transcriptional control of genes involved in apoptosis by addressing the cooperative influence of diverse regulatory partners, pivotal in unraveling the mechanisms leading to tissue-wise regulation of biological processes.