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گوشِ عالم میں پڑی ہے وَرَفَعنا کی صدا
ہر زمانے نے سنی ہے وَرَفَعنا کی صدا
گلشنِ جاں میں رچی ہے ’’لَکَ ذِکرَک‘‘ کی مہک
دل کی دھڑکن میں بسی ہے وَرَفَعنا کی صدا
مالکِ خیر نے کی خیر کی کثرت اُنؐ پر
اہلِ شر سے نہ دبی ہے وَرَفَعنا کی صدا
عقل والوں نے جسے ذکرِ خدا سمجھا ہے
عشق والوں میں وہی ہے وَرَفَعنا کی صدا
مدحتِ شاہِ اُممؐ زینتِ قرطاس و قلم
صوت کا حسن بنی ہے وَرَفَعنا کی صدا
سننے والوں نے سنی آیۂ نَشرَح لیکن
لبِ قاری پہ سجی ہے وَرَفَعنا کی صدا
اس پہ قرآن کی آیات ہیں شاہد عرفاںؔ
ربِّ کونین نے دی ہے وَرَفَعنا کی صدا
Background: Sleep is an essential function of our body. Many surveys have reported the prevalence of poor sleep in university students, especially in medical students. Objectives: The objective of the study was to evaluate the effect of physical activity and stress on sleep quality among medical students in Pakistan. Materials & Methods: An observational cross-sectional study was conducted on medical students of private medical colleges in Lahore. A convenient sampling technique was used and 210 students were selected. The Pittsburgh Sleep Quality Index (PSQI), Godin Shephard Leisure Time Physical Activity Questionnaire (GSLTPAQ), and Perceived Stress Scale (PSS 10) were used for data collection. We used SPSS version 20 to analyze data and applied statistical tests: Chi-square test and Logistic Regression. p-value < 0.05 was taken to establish significance. Results: Among the study participants 91(43.3%) were males and 119 (57.7%) were females. There was a significant effect of stress level on sleep quality (P=0.000*). The frequency of good sleepers was seen to increase by almost three times with increasing physical activity, however, this difference remained non-significant (p=0.07). The logistic regression test showed a significant relationship between poor sleep and stress (p=0. 008**) while no significant relationship was seen between sleep quality and physical activity. Conclusion: There was a significant association between poor sleep and high-stress levels and an increase in physical activity showed an increase in the frequency of good sleep, however, this difference was non-significant. It can be inferred that this positive effect of increasing physical activity on the quality of sleep could be indirectly due to its relieving effect on stress.
The present study has been designed to investigate the antioxidant and antimutagenic potential of hydrolyzed and non-hydrolyzed extracts of twenty medicinal plants belonging to different plant families like Amaranthaceae, Cucurbitaceae, Labiatae, Fabaceae, Solanaceae, Poaceae, Malvaceae and Zygophyllaceae. Hydrolyzed extraction was carried out using acidified methanol and non-hydrolyzed extracts were obtained with aqueous methanol. Both types of extracts were evaluated for phenolic contents, antioxidant, mutagenic and antimutagenic potential and genoprotective activity. Hydrolyzed extracts of all plants represented higher phenolic contents, antioxidant and antimutagenic potential as compared to non-hydrolyzed extracts. Mentha longifolia (402.8±1.9 mg GAE/g dry extract), Citrullus colocynthus (396.4±1.9 mg GAE/g dry extract) and Mimordica dioca (354.4±1.9 mg GAE/g dry extract) showed highest phenolic contents. Cassia angustifolia (230.4±2.6 mg AAE/g dry extract), Mimordica dioca (132.6±0.8 mg AAE/g dry extract) and Mentha longifolia (131.7±1.5 mg AAE/g dry extract) presented largest reducing power in terms of ascorbic acid equivalents (AAE). Highest free radical scavenging was shown by Citrullus colocynthus (96.21±1.0%; IC50 2.11±0.12 µg/mL), Achyranthus aspera (93.1±0.4%; IC50 6.5±0.10 µg/mL), Mentha longifolia (92.4±1.0%; IC50 14.3±0.1 µg/mL). Citrullus colocynthus (96.21±1.0%; IC50 2.11±0.12 µg/mL), Mimordica dioca (91.91±1.0%; IC50 17.22±0.36 µg/mL) and Mentha longifolia (92.4±1.0%; IC50 14.3±0.1 µg/mL) exhibited highest antioxidant activity in terms of inhibition to lipid peroxidation. Lowest cytotoxicity was observed for Citrullus colocynthus (2.65±0.09%), Achyranthus aspera (2.95±0.14%) and Mentha longifolia (3.95±0.08%) by evaluating haemolytic activity. Highest antimutagenic potential was calculated for hydrolyzed extracts of Citrullus colocynthus (75.79±0.69%), Mimordica dioca (73.02±1.37%) and Achyranthus aspera (72.22±0.69%). Also, Citrullus colocynthus, Mimordica dioca and Achyranthus aspera showed considerable protection against oxidatively damaged DNA plasmid i.e. pBR322. Finally, it has been concluded that Citrullus colocynthus, Mentha longifolia, Mimordica dioca and Achyranthus aspera were the most bioactive plants of all selected medicinal plants as represented by the results of LCMS analysis that exhibited the presence of high concentrations of bioactive compounds in the extracts of these plants.…………………………………