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Isolation and characterization of cytolytic protein gene from local isolates of bacillus thuringiensis

Thesis Info

Author

Rabia faiz

Department

Department of Zoology

Program

PhD

Institute

Government College University

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Degree Starting Year

2011

Degree End Year

2014

Subject

Zoology

Language

English

Other

CD is also available at PG Library

Added

2021-02-17 19:49:13

Modified

2023-01-06 19:20:37

ARI ID

1676711125850

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اظہارِ تشکر

اظہارِ تشکر

حمد و ثنا ہے اُس خالقِ لم یزل ولا یزال کے لیے جو لائقِ تسبیح و تمجید ہے۔جس نے اپنی قدرتِ کاملہ سے ذرے کو آفتاب،قطرے کو قلزم  اور عظیم آسمان کے شامیانے کو بغیر کسی سہارے کے نصب فرمایا۔جس کا کمال اس کی معرفت ہے اور جس کا شاہ کار "لولاکِ لما خلقت الافلاک" کا مصداق ہے۔لاتعداد درود و سلام اُس ہادی برحق معلمِ قدس ،سید الانبیاء  حضرت محمد مصطفیٰ  صلی اللہُ علیہ وآلہ ٖ وسلم کی ذاتِ مقدسہ و مطہرہ پر۔اور لاکھوں درود و سلام صاحبانِ کساء ،اہلبیتِ اطہار ؑ اور اصحابِ باوفا رضوان اللہ علیھم اجمعین کی ذواتِ پاکیزہ پر۔

یہ مقالہ میری زندگی کی اولین تحقیقی کاوش اور میری زندگی کے خوابوں  کی ادنی سی تعبیر ہے۔جس کے لیے میں نے ان تھک محنت کی۔ الحمد للہ میری اس کاوش کو خدائے بزرگ  و برتر نے  تکمیل  تک پہنچانے میں میری مدد فرمائی۔

       میں شکر گزار ہوں اپنے عظیم والدین کا جن کی بے پناہ محبتوں اور شفقتوں کے سبب میں ا س قابل بنا۔ جنہوں نے زندگی میں ہر گاہ رہنمائی فرمائی اور اپنی دعاؤں کے سائے میں پروان چڑھایا۔میرے والدِ بزرگ وارسید محمد حسین شاہ صاحب  کو تعلیم سے بے پناہ محبت ہے۔اُن کی زندگی کی یہ خواہش رہی ہے کہ ہمیں بہترین انداز میں زیورِ تعلیم سے آراستہ فرمائیں۔آج میں فخر محسوس کر رہا ہوں کہ ان کے خواب کو تکمیل تک پہنچانے میں خدا نے میری مدد فرمائی۔خدا میرے والدین کو سلامت رکھے اور ان کی محبتوں کا ابر ِ رحمت ہمیشہ برستا رہے۔

میں اپنے محسن و مشفق استاد نگرانِ مقالہ پروفیسر ڈاکٹر سید اشفاق حسین بخاری کا شکر گزار ہوں جن کے علمی سایہ عاطفت میں یہ تحقیق  مکمل کی ۔اچھی...

Development of Micro, Small and Medium Enterprises (Msmes) Based on Creative Economy (Ekraf) and Gig Economy in Gorontalo Regency

The purpose of this study is to determine the development of MSMEs in Gorontalo Regency and to provide new innovations in the development of MSMEs based on Creative Economy (Ekraf) and Gig economy in Gorontalo Regency. This study uses a descriptive qualitative approach, which describes and describes facts according to the phenomenon of how development is seen from the strengths, weaknesses, threats and opportunities (SWOT analysis) with the aim of providing an Creative Economy-based MSME development strategy and the Gig economy in Gorontalo Regency. Data collection was carried out through observation, interviews and documentation about the strengths, weaknesses, opportunities, and threats of MSMEs against informants from three business fields, namely the Food Business Sector (dodol processed food), clothing (Sulaman Karawo) and handicrafts spread across three existing sub-districts in Gorontalo Regency. The results showed that the creative economy-based and gig economy-based MSMEs in Gorontalo Regency have not been fully implemented by MSME actors because they have limited capabilities and experience problems in their business development, this is due to several problems faced such as limited capital, raw materials and the use of simple production equipment, limited marketing media due to the absence of a MSME center, the types of products offered, quite a lot of transaction costs, less skilled labor, and product copyright registration problems for that from the results of the SWOT analysis of the diversification strategy at a more precise value to be applied, because it is considered more appropriate to the current conditions in Gorontalo Regency. In addition, the use of the gig economy is a part-time worker who is competent and innovative for MSMEs that are progressing and ready to compete.

Effect of Various Grain Storage Structures and Temperature Stress on Seed Quality and Germinability of Different Wheat Varieties

Grains are among the most important staple food and Pakistan has made a significant advance in increasing grain yield through the introduction of high yielding genotypes including new packages of production technologies. However, care is to be taken for storage conditions and storage periods for maintaining nutritive values, seed viability and vigor. This study therefore was conducted to determine how storage sources, storage periods and temperature regimes affect physical and chemical properties, and germinability traits of seeds of various wheat varieties. Laboratory experiments were conducted at Department of Agronomy, Sindh Agriculture University, Tandojam, Pakistan, located at (25o25’60’N 68o31’60’E) during 2008 and 2009. The study on seed quality assessment of different wheat varieties in various seed storage structures consisted of five wheat varieties (Moomal- 2000, TJ- 83, Imdad-2005, Abadgar-93 and Mehran-89), five storage sources (Jute bags kept under open sky covered with plastic, jute bags kept in closed storage, plastic bags kept in closed storage, earthen silos, and iron bins), and two storage durations (90 and180 days). The maximum seed index was observed for Imdad-2005 stored in iron bins. Seed moisture content was also higher in Moomal-2000 stored in iron bins, however, germination was superior in Moomal-2000 stored in earthen silos, and or plastic and jute bags kept in closed storage, or under open sky covered with plastic sheets. Higher protein content was found in Moomal-2000, TJ-83, Mehran-89 and Imdad-2005 stored in jute bags kept in closed storage or under open sky covered with plastic sheets. Wet gluten was greater in seeds of Mehran-89 and Abadgar-93 stored in jute bags kept in closed storage or under open sky covered with plastic sheets. Starch and ash contents were better in Moomal-2000 stored in jute bags kept under open sky covered with plastic sheets or in iron bins. Lipid content and falling number were more in TJ-83 stored in all types of storage sources. EC was higher in seed of Moomal-2000 stored in all types of storages. The seed N accumulation was more in TJ-83 and Mehran-89 stored in jute bags kept under open sky covered with plastic sheets or kept in closed storage, whereas, seed P and K contents were superior in Moomal-2000 stored in various storage sources. Minimum seed index was observed in variety TJ-83 stored in any type of storage source, moisture content in Mehran-89 kept in jute bags placed in open sky. However, lowest germination, protein and wet gluten percentage were recorded in variety Abadgar-93 stored in iron bins and jute bags stored in closed stores respectively, starch in Abadgar-93 stored in plastic bags, ash in TJ-83 kept in jute bags (covered with plastic sheets) and lipids in Mehran-89 stored in iron bins. The lower EC and falling number were noted in Mehran-89 and Abadgar-93 stored in jute bags and placed under open sky covered with plastic sheets and closed stores.The study on effect of different temperature regimes on the germinability of different wheat varieties revealed highest germination in Abadgar-93 kept at 30oC, shoot length in Moomal-2000 and Mehran-89 was higher when kept at 20 and 30oC, respectively. Root length, fresh shoot weight, fresh root weight, and dry root weight were recorded higher in variety Mehran-89 kept at 30oC. Seed vigor index is the concept where evaluation is made on the germination percentage and seedling growth within first 6-7 days and variety Mehran-89 proved more vigorous than rest of varieties. It is concluded that all the wheat varieties were found suitable for milling, bread, chapatti and yeast leavened bread containing adequate protein, starch, ash, wet gluten, lipids etc. The information obtained in this study is useful for researchers, farmers, millers, bakers and daily cereal users for the selection of suitable variety. Regarding storage sources, iron bins are recommended for seed storage for maintaining physico-chemical properties other than seed purpose. For the seed purpose, earthen silos, and or plastic and jute bags kept in closed storage, or under open sky covered with plastic sheets are appropriate seed storage sources. It is further recommended that temperature ranging between 20 and 30oC is the optimum temperature regime for wheat seed germination and related traits.