قائد کا فرمان کام ، کام اور کام
نحمدہ وَ نُصَلِّیْ علی رسولہ الکریم امّا بعد فاعوذ بااللہ من الشیطن الرجیم
بسم اللہ الرحمن الرحیم
معزز اساتذہ کرام اور میرے ہم مکتب ساتھیو!
آج مجھے جس موضوع پر اظہار خیال کرنا ہے وہ ہے:’’قائد کا فرمان کام، کام اور کام‘‘
جنابِ صدر!
اس کا ئناتِ رنگ و بو میں جو رنگینیاں نظر آرہی ہیں، اس کائنات میں جوحسن نظر آرہا ہے اس کائناتِ رنگ و بو میں جو تنوع دکھائی دے رہا ہے، اس کائنات کے گلشن میں جو بہار آ ئی ہوئی ہے، یہ سب کچھ اپنے آپ وجود میں نہیں آگیا، یہ کسی نہ کسی کاریگر کا شاہکار ہے، کسی نہ کسی محنتی کی محنت ہے۔
جنابِ صدر!
محنت اور جدوجہد ہی انسان کو انسانیت کے مقام پر فائز کرتی ہے، معاشرے میں اس کا ایک مقام بناتی ہے، کام سے محبت ہی بڑے بڑے کا م بناتی ہے، کام کرنے والا شخص کسی کا دست نگر نہیں ہوتا، اُس کی خودی میں اضافہ ہوتا ہے، اُس کی خودداری کی دیوار میں شگاف نہیں پڑتا، وہ غریب اور بے کس کی دستگیری بھی کر سکتا ہے۔
بے محنت پیہم کوئی جوہر نہیں کُھلتا
روشن شرر تیشہ سے ہے خانۂ فرہاد
صدرِذی وقار!
بانیٔ پاکستان قائدِ اعظم محمد علی جناح رحمۃ اللہ علیہ کا یہ فرمان زبان زدعام ہے کہ کام ، کام اور کام، اس نابغۂ روزگارہستی نے کام کر کے یہ ثابت کر دیا کہ اگر انسان کا ارادہ نیک ہو، انسان کے جذبات حق وصداقت کے ترجمان ہوں، اور مقصد نیک ہو، عوام النّاس کی خدمت کا جذبہ موجزن ہو تو مقصد کے حصول میں کوئی چیز رکاوٹ پیدا نہیں کرسکتی۔
صدرِذی وقار!
قائدِاعظم محمد علی جناح رحمۃ اللہ علیہ نے جب یہ فرمایا، کہ کام سے تعلق پیدا کرو،...
Dealings of the Holy Prophet (S.A.W) with non-Muslim show that Islam is the religion of peace, brotherhood, religious tolerance, independence and humanity. Before the spread of Islam the conversations and dealings of the Holy Prophet with non-Muslims forced them to call him Al-Sadiq and Al-Ameen. Sympathy and tolerance in clandestine and public preaching, the style of letters to non-Muslim empires, the dialogues with non-Muslim delegations in Madani era, the orders given for the safety of their wealth, belongings and their life, the tolerance, forgiveness and prayers for the enemies, the good behavior with the non-Muslim prisoners, even when Muslims got dominance over non-Muslims at the time of the conquest of Makkah, the common pardon given by Holy Prophet (S.A.W) are the true example that Islam preaches peace. The attitude of Sahaba-e-Karam (R.A) and the liberty of rights given to Non-Muslims by them- no religion and no community of the World can give this example. The people responsible for maintaining the peace of the world and brotherhood should see how Muslims are treated all over the world? Muslims had given to non-Muslim permission to practice their religious festivities freely and even to wear any type of clothes. The ban of Hijab in France, the punishment of innocent Dr. Afia Siddiqui, the Egyptian pregnant woman (Marwa Sharbeney) had been mercilessly assassinated and her husband injured by knives in the court of Germany because of Hijab, the discussions going on to restrict the domes of Mosques and Adhan (Call to prayer) in Switzerland, the insult of the Holy Quran by Pope Terry John, of Florida, the disrespect of Holy Prophet (S.A.W) etc are the questions awaiting answers from West and United Nation. Islam promotes of peace, equality and religious freedom. It also invites non-Muslims to come and sit on a platform and discuss these problems for the sake of worldly peace to end the religious hatred and to form true peace in the world.
CMCases are important enzymes that can hydrolyze lignocellulosic mass efficiently. Apart from this, CMCases are used in industries pertaining to textile, food processing, wine and brewery, pulp & paper, animal feed, agro based products, detergents, waste management, olive oil extraction and carotenoid extraction. The aim of this study is to explore the diversity of the indigenous thermophilic bacteria that are capable of degrading cellulosic biomass. A total of thirty-seven bacterial strains were isolated from stove ash samples. All the isolates were Gram positive and on the basis of physico-biochemical characterization identified and named as Bacillus sp TLW-1 to TLW-37. Plate screening was performed for ten different industrially important hydrolytic enzymes. Almost all the isolates were able to produce all the tested enzymes (except pectinase) with different profile. Positive CMCase producers were quantitatively screened by shake flask method. The isolate Bacillus sp. TLW-3 showed the highest CMCase production and was selected for further study. Isolate was identified on the basis of 16S rDNA sequencing as Bacillus licheniformis TLW-3. Sequence was submitted to NCBI (with KY440432 accession number). According to the growth curve, the isolate entered into log phase after 6 hrs of incubation and the log phase ended after 30 hrs incubation. Afterwards, the stationary phase began which ended after an incubation period of 72 hrs then the decline phase started. Highest enzyme production was found during the stationary phase. Optimization of production and growth conditions was performed by using two techniques i.e. OFAT and RSM. According to the OFAT approach, the highest growth and CMCase production were obtained in the medium containing 1% CMC, 1% peptone and 0.5% yeast extract with pH 7.0 at 50˚C and 150 rpm after the incubation period of 72 hrs. When compared with RSM optimization, the best growth was found at 70˚C in the medium having 0.5% peptone, 2.3% yeast extract and 2.52% CMC with pH 9.0 after 49.2 hrs of incubation. RSM optimized production conditions for highest CMCase production was found to be 1.8% peptone, 1.4% yeast extract and 1.6% CMC with pH 7.0 at 70˚C and 69.63 hrs of incubation period. Co-production of the enzyme was obtained in the medium containing 1% CMC and 1% starch as the sole carbon source. For the purification purpose, various precipitation and chromatography techniques were employed. CMCase was successfully (partially) purified with 8.250-fold and 13.75 % yield. Molecular weight of CMCase was estimated to be 28 kDa. Enzyme kinetics of partially purified CMCase demonstrated that enzyme was found active in the reaction mixture containing 1% CMC with pH 7.0 and at temperature 70˚C in the presence of Co+2, Ca+2 and Hg+2 ions. When the glucose was added in the reaction mixture enzyme activity was inhibited. The stability conditions of CMCase include, pH 6.0 and at temperature 4-50˚C; however, the stability was totally lost in the presence of the tested metal ions. The Vmax, Km and activation energy for CMCase were found to be 42.553 IU/ml/min, 9.587 mg/ml and 2.649 KJ/mole respectively. Endoglucanase gene was amplified, sequenced (11888 bp) and submitted to NCBI (with MF953225 accession number). Expasy-protparam online server was used for amino acid translation and in slicio physico-chemical characterization of the sequence. Secondary and tertiary structure of the protein revealed using SOPMA and ITASSER online server respectively.