پروفیسر حبیب الحق ندوی
فروری کا معارف چھپ چکا تھا کہ پروفیسر حبیب الحق ندوی کے انتقال کی اندوہ ناک خبر ملی۔ وہ باڑھ (پٹنہ) کے سادات کے ایک معزز خاندان سے تعلق رکھتے تھے، مدرسۃ الاصلاح سرائمیر میں میری طالب علمی کے زمانے میں ان کے دوچھوٹے بھائی بھی تعلیم حاصل کررہے تھے جن سے ملنے کبھی کبھی حبیب الحق صاحب بھی آجاتے تھے، وہ خود بھی یہاں زیر تعلیم رہ چکے تھے، پھر پاکستان چلے گئے اور عرصہ سے جنوبی افریقہ کی ڈربن یونیورسٹی سے وابستہ تھے، مولانا سید ابوالحسن علی ندوی مدظلہ سے بیعت تھے، ان سے ملنے لکھنؤ آتے رہتے تھے، اسی سلسلے میں ایک دفعہ بے سان و گمان دارالمصنفین بھی پہنچے، لکھنؤ سے اعظم گڑھ کا یہ سفر بڑا مشقت طلب رہا مگر دارالمصنفین کو دیکھتے ہی ان کی ساری کلفت دور ہوگئی، سرائمیر جانے کی خواہش کی جہاں کی سادگی اور اپنے زمانہ کے استاذوں کے ایثار و قناعت کے بڑے مداح تھے۔ ایک اور دفعہ ندوۃ العلماء میں روس کی آزاد مسلم ریاستوں کے بارے میں میں نے ان کا پُراز معلومات خطبہ سُنا تو انہیں اعظم گڑھ آنے کی دعوت دی یہاں بھی ان کی تقریر بہت پسند کی گئی جس سے ان کے علم و مطالعہ کی وسعت، حالاتِ حاضرہ سے باخبری اور ملی درد کا اندازہ ہوتا ہے۔ دارالمصنفین سے ان کو قلبی لگاؤ تھا، معارف پابندی سے پڑھتے، اس میں ان کے مضامین اور خطوط بھی شایع ہوتے تھے، اپنا سہ ماہی انگریزی رسالہ اور تمام کتابیں یہاں بھیجتے، ادھر عرصہ سے ان کا حال معلوم نہیں ہوا تھا، دفعتہ ان کے ارتحال کی خبر آگئی۔ اﷲ تعالیٰ غریق رحمت کرے اور متعلقین کو صبر و شکیب بخشے، آمین۔ (ضیاء الدین اصلاحی، مارچ ۱۹۹۸ء)
Islam is a complete code of life and provides the rights to every class of the human beings. Women rights is a kind of such basic rights which were not bothered in the world but Islam provided it to this gender in its ancient age. So many enactments have been made in Pakistan at federal and provincial level. “Punjab Protection of Women Against Violence Act 2016” will be main study of this research article in which. This act was passed rapidly without any detailed discussion on it, so is the reason that it bears so may deficiencies in it. Implementation of this act will surely cause to create the internal problems in family life and will destroy the family system of the era. Some provisions of this act are repugnant to Islamic teachings as well as to ethical norms which make the husband helpless, notorious and such sinful and criminal person who has no right of honor and respect in the society and this will become a permanent document of dishonor which will affect his person as well as his whole family. These main points of this Act will be analyzed in sharia perspective in this research paper.
Poultry industry is a dynamic sub-division of agriculture sector throughout the world as well as in Pakistan owing to rising global demand for this more affordable animal protein than red meat, due to exponential population growth and rapid urbanization in developing regions. However, the major problems encountered by the industry are infectious viral diseases; mainly infectious bursal disease (IBD), causing severe production and economic losses. Currently, live, attenuated or killed classical virus strains are used as vaccines to immunize birds but still outbreaks are reported even in vaccinated flocks probably owing to difference in antigenic structure between vaccine and field vvIBDV strains. Also, the current vaccine production systems including microbial, stable nuclear and transient plant viral technologies are not feasible in terms of cost and biosafety. So, as a preliminary step for development of clean quality and cost-effective recombinant subunit vaccine, a VP2 gene along with engineered choloroplast based Prrn promoter and PsbA terminator was synthesized commercially. Phylogenetic analysis and multiple sequence alignment with representative strains of different geographic origin confirmed the antigenic structure similarity of synthetic VP2 protein with those of circulating vvIBDV strains. Synthetic VP2 gene together with FLARE-S gene cassette was flanked by tobacco chloroplast flanking sequences through sequential cloning leading to development of final chloroplast transformation construct. Fully expanded 4-6 weeks old tobacco leaves were bombarded using a gene gun for chloroplast transformation. Initially, putative transplastomic lines regenerated on spectinomycin selection medium were selected by visual detection through gfp fluorescence as transplastomic sectors in a chimeric leaf tissue fluoresced green under UV light. These fluoresced sectors not only revealed heteroplasmic state of transgenic plants but also confirmed the successful integration of marker gene into tobacco chloroplasts. Putative transplastomic shoots were then subjected to additional rounds of selection and regeneration for developing homoplasmic clones. The genetic analysis of putative transplastomic plants through polymerase chain reaction confirmed the presence of VP2 as well as aada and gfp genes in the plastome. Integration of cassettes into inverted repeat region was confirmed using forward primer specific to aada gene and reverse primer specific to plastome. Further, development of an efficient regeneration system for soybean via somatic embryogenesis was attempted using 2 and 7 days old mature cotyledons from in-vitro germinated seedlings of a local ‘Faisal’ and an exotic ‘Jack’ cultivar with the intention to develop edible VP2 subunit vaccine as soybean is a chief constituent of poultry feed. It was observed that 7 days old mature cotyledonary explants of cultivar Jack were more responsive towards somatic embryogenesis than 2 days old ones and those of cultivar Faisal after callus induction on D20 medium directly. However, no regeneration was observed in either case indicating the need for further experimental investigation