The Importance of Emerging Sciences & Technology in the Light of Quran & Prophetic Teachings

Science and technology is on top position contemporary now it is need of our masses to compare these formulas with holy Quran because Quran is miracle and implementable till the day of  Judge ment  lot of scientists have benefited by Quran and they also agree up on the excellence of Quran. Hence in this research we have to prove dominancy of Quran over on the science and technology the hints of new inventions and discoveries are available in Quran if we study the Quran deeply we will find in it complete agreement with modern science however it was revealed in that time people were not aware from scientific inventions and they have not know-how of it. Comparatively this time is well advanced period and lot of things invented while concept of these things was present in this sky book I have drawn intention of readers on these points. In this research I would discussed that importance of science and technology specially the sub subjects of the modern science math and statistics geology, astronomy, space sciences, Embryology, Genetics and computer technology. It should be remember that the exposition of this book is also two types scientifically and logically because Quran invites us towards intelligence and wisdom.   It is responsibility of scholars to interpret the Quran contemporary because this is not merely ancient book but it is for all times and not for Arabs but for guide line of whole humanity of entire universe. It is challenges itself that no any kind of error or mistake in it foes of Islam tried to find faults but they not succeeded to do so resulting they affirmed dignity of it over on all the books.

Evaluation of Potential Biotic Stress Resistance Genes Form Gossypium Arboreum

Cotton is the most important fibre crop of the world. Pakistan is the fourth major contributor in cotton production. In our country a viral disease caused by cotton leaf curl virus (CLCuV) is the most important factor limiting cotton production of widely cultivated Gossypium hirsutum varieties since the induction of the disease in this region. Gossypium arboreum is the cotton species that is resistant to this disease. Cotton scientists are working to find the key genes in G. arboreum that confer resistance against cotton leaf curl disease (CLCuD). The present study is also an effort to find some potential biotic stress resistance genes from G. arboreum and the evaluation of some genes against CLCuV infection. Different approaches were employed to identify some potential biotic stress resistance genes from G. arboreum. These approaches include screening of G. arboreum leaf cDNA library, finding homologs of resistance genes in the ESTs database of G. arboreum and their screening in G. arboreum and CLCuV symtomatic and asymtomatic plants of G. hirsutum genotypes, cloning of resistance genes from G. arboreum and G. hirsutum using degenerate primers approach and isolation of full length putative biotic stress resistance genes from G. arboreum in RT-PCR. The identified full length genes were coding full length allene oxide synthase, hydroperoxide lyase and calreticulin proteins that were cloned in plant expression vector and were transformed in Nicotiana tabacum through Agrobacterium mediated transformation. The T1 generation of transgenic plants was inoculated with cotton leaf curl multan virus (CLCuMuV) through leaf infiltration. The virus presence and titre in inoculated plants was determined through conventional and realtime PCR respectively. No significant difference in virus titer was detected in all transgenic plants with respect to control plants. It is inferred that resistance against CLCuV might not be due to single gene. Multiple genes might be involved in conferring resistance against this disease. The various approaches adopted in this study to identify possible resistance genes against CLCuV infection resulted in identification and isolation of a set of genes. Theses genes are a valuable source to study their interaction against various coton pathogens and can be utilized to improve resistance level of susceptible G. hirsutum and other plant species.