ہک دھی رانی دی فریاد
بولے جدوں بنیرے کاں
میں سمجھ جاندی ہاں
گل ہے ضرور اولی
تاہیوں کردا اے کاں کاں
کائی دس پیغام خوشی دا
مینوں درداں ماریا تھاں
میں کٹھی وچ ہجر دے
میری نکلی جاندی جاں
میرے سینے پھٹ انوکھا
کر سکدی نہیں عیاں
میری سن فریاد اے امبڑی
جے توں ہیں میری ماں
نہیں سُجھدے ریشم گوٹے
نہ محل چوبارے تھاں
نہ چوڑے ہار حمیلاں
نہ کنٹھا منگدی ہاں
نہ گانی نتھ نہ ِٹکّا
نہ منگاں حویلی تھاں
نہ ریشم لہنگے منگاں
نہ سونا چاندی چاہاں
نہ ہور قصیدے چوڑے
نہ قریشیے بانہاں
نہ مربعے، بھوئیں نہ بھانڈے
نہ کوئی لمبی……… لاں
نہ ریجھ مایا دی مینوں
نہ مجھ نہ وچھا گاں
ہک راز دلے وچ میرے
دس میں ہن کی کراں
تیرے لکھ احسان کروڑاں
بھل سکاں میں کداں
تیرا حکم میرے سر اکھیں
توں سکی میری ماں
اک خیر منگاں میں تیتھوں
نالے منگدی وی سنگاں
کر سکدی توں ہیں اماں
میری زندگی میرے ناں
جے میری گل توں منیں
میں اُڈّاں باہجھ پراں
جے نال رنجیٹھے ٹوریں
دل ٹھردا میرا تاں
Current scenario of newer diseases with multiple causes has drawn the attention of the researchers in the field of therapeutics and they are now inclined to identify molecules effective for targeted therapy. Objective: Quinoline (1-azanaphthalene); belongs to heterocyclic aromatic nitrogen compound. Some quinoline-based derivatives are also known for their anti-tumor activity. The study was planned to evaluate the cytotoxic potential of quinoline derivatives. Methods: Berberine; a quinoline compound was made part of study to make structural analogs which were docked against potential target proteins. Cytotoxic profiling of all derivatives was done using MTT cytotoxicity assay. Results: The pharmacoinformatic and structure activity relationship studies of analogs were done. The cytotoxic profiles were elucidated by comparing viability rates of analogs treated hepatic cancerous cell line with untreated hepatic cells and untreated mesenchymal stem cells as standards. Marked cytotoxicity was seen in all molecules at low doses than reported in past studies with relevance to parent compound. Conclusions: The results will be further confirmed through various other cell culture assays targeting different marker proteins, pharmacoinformatics tools and structure activity relationship studies
The present investigation deals with the establishment of an efficient in vitro selection strategy to produce salt-tolerant cell lines and subsequent regeneration protocols in potato (cvs. Cardinal and Desiree). The activities of antioxidant enzymes (peroxidase, catalase and superoxide dismutase) and total soluble protein contents of various tissues under stress were evaluated to understand their possible role in salinity tolerance. Exogenous application of ascorbic acid and salicylic acid were also tested for salt stress alleviation. In order to proceed with these objectives, the initial focus was to establish protocols for micropropagation, callus induction and maintenance, plant regeneration, establishment of cell suspension cultures and ex vitro acclimatization of regenerated plants. Three different concentrations of TDZ (10-8, 10-9, or 10-10 M) in MS medium were tested for the purpose of in vitro clonal propagation. MS basal medium fairly supported micropropagation of both the tested potato cultivars followed closely by MS medium supplemented with TDZ (10-10 M). For callus induction and proliferation in dark, internodal segments proved to be a good explant source whereas MS medium fortified with 2, 4-D (18.09 μM) was the best medium composition equally effective for both the potato cultivars. A combination of NAA (2.64 μM) and TDZ (1.00 μM) supplemented to MS medium was the best choice for shoot initiation from callus cultures after 20 and 21 days in Cardinal and Desiree, respectively. Rooting of regenerated shoots was achieved on MS medium supplemented with 8.87 μM BAP, 2.64 μM NAA and 0.123 μM IBA. Cell suspension cultures using friable calluses were developed successfully using MS2 medium for the two cultivars. The best supporting medium for ex-vitro transplantation of potato plants was vermiculite. It was observed in this study that different in vitro growth parameters, i.e., shoot/root length and numbers of roots decreased while number of shoots increased with an increase in NaCl (20-140 mM) concentration in the medium. In Desiree, rosette-type of shoot development initiated at 100 mM whereas in Cardinal it was evident at 120 mM NaCl level. During this investigation, a direct recurrent selection procedure was employed to select salt- tolerant cell lines in potato (Cvs. Cardinal and Desiree) on the basis of sub-lethal concentration of salt. Results have shown more than 50% reduction in relative fresh weight in both the cultivars above 100 mM NaCl. Callus morphology correspondingly changed from off-white to blackish-brown above 100 mM to acutely-necrotic at 140 mM NaCl. Regeneration potential of recurrently-selected callus cultures (100 mM NaCl-treated) on salt- free medium was more pronounced in Desiree as compared to Cardinal. When well- acclimatized recurrently-selected plants were treated with 100 mM NaCl and compared with control plants to check their acquired salinity tolerance, it was observed that recurrently- selected plants showed higher fresh/dry weight and number of tubers in both the cultivars. A slight decrease in protein contents of in vitro Cardinal cultures was observed as the concentration of NaCl (20-140 mM) gradually increased in the media. However, there was an increase in protein contents in Desiree plants when subjected to increasing salt concentrations. In case of in vitro recurrently-selected plants, protein contents were higher as compared to control (non-selected ones) in both the cultivars. The peroxidase activity exhibited a slightly decreasing trend in Cardinal though an increasing one was observed in Desiree with an increasing NaCl level in the medium. In the present investigation, recurrently-selected plants had higher POD, CAT and SOD activities as compared to the control ones in both the cultivars.