مولانا حافظ احمد سعید دہلوی
قلم یہیں تک پہنچا تھا کہ اچانک مولانا حافظ احمد سعید صاحب دہلوی کی وفات حسرت آیات کی اطلاع ملی۔اناﷲ وانا الیہ راجعون۔مولانا جمعیۃ العلمائے ہند کے بانیوں میں سے تھے اوراس کے پہلے جنرل سیکرٹری تھے۔ اس حیثیت سے انھوں نے ملک میں دورے کیے اورجگہ جگہ تقریریں کیں،یہ زمانہ تحریک خلافت کے شباب کازمانہ تھا۔مولانا کی تقریروں نے دھوم مچا دی اوربچہ بچہ کی زبان پران کانام تھا۔دلّی کی ٹکسالی زبان بولتے اوراسی میں گھنٹوں خاص لب ولہجہ کے ساتھ تقریر کرتے تھے۔اس لیے اُن کی گفتگو اورتقریر دونوں اس قدر شیریں اورشگفتہ ہوتی تھیں کہ بس ’’وہ کہیں اورسُنا کرے کوئی‘‘۔ قرآن مجید کے بہت اچھے حافظ تھے اوراُس کے ساتھ بڑا شغف رکھتے تھے۔نہایت آسان زبان میں قرآن کاترجمہ وتفسیر لکھ رہے تھے جو غالباً پایۂ تکمیل کو پہنچ گئی ہے۔ دینیات پرمتعدد کتابیں بھی ان کی یادگار ہیں۔ متعدد بارحریت وطن کی راہ میں جیل گئے۔ طبعاً بے حد خلیق، ملنسار، خوش طبع اوربڑے بذلہ سنج بزرگ تھے۔ اگرچہ ادھر دوبرس سے جمعیتہ کے صدر تھے لیکن چند درچند عوارض واسقام کے باعث برسوں سے گوشہ نشین ہوگئے تھے۔دلی سے باہر کے لوگ اُن کی تقریر سننے کو ترستے تھے مگرانھوں نے سفر کرنا بالکل ترک کردیا تھا۔عجب باغ وبہار انسان تھے۔ان کا فقرہ فقرہ زبان کی لطافت ونفاست کامرقع ہوتا تھا۔دلّی وہ دلّی ہی نہیں رہی ہے۔ اب ایسے بزرگ کہاں ملیں گے؟کل من علیھا فان ویبقیٰ وجہ ربک ذوالجلال والاکرام۔ اﷲ تعالیٰ کروٹ کروٹ جنت نصیب اورصدیقین وشہدا کامقام جلیل عطافرمائے۔آمین [دسمبر۱۹۵۹ء]
Islam is religion of humanity that wants us to behave good to others and has nothing to negate the rights of others. Minorities also have their legal rights in a welfare society, which makes itself an examplary society. Islam is a religion that negates violence. The rights of minorities can be traced in islamic society by many saying of Holy Prophet ﷺ. Holy Prophet ﷺ always advised muslims to treat the minorities kindly. Whatever the deligation of minorities came, Prophet ﷺ hosted himself. In the same way, when a deligation came from Najran, Prophet ﷺ not only hosted but allowed them to worship in Masjid al Nabi according to their own religion. In the light of ordinance of Prophet ﷺ, even fourteen centuries have passed but minorities are being given their rights. According to the law and order of a welfare state, minorities must be given their rights. In this article, the rights of minorities in a welfare state are tried to discuss.
Glioblastoma multiform (GBM) and Non-small cell lung cancer (NSCLC) are most invasive and uniformly fatal type of brain and lung cancer respectively, with median survival of less than 20 months after diagnosis even with the most aggressive treatment that includes surgery, radiation, and systemic chemotherapy. Currently many chemotherapeutic anticancer drugs are being used in clinical trials which inhibit tumor growth by inhibiting certain pathways inside cancer cells. Luteolin and Ellipticine are plant derived compounds of potent antitumor activity, class of topoisomerase II inhibitor which intercalates with DNA and makes DNA adduct and kill cancer cells. Another class of drugs is polo-like kinase (PLK) inhibitors; among those BI-2536 is highly potent anticancer PLK inhibitor with IC50 of less than 0.9 nM is recently abandoned from phase II trials due to adverse neutropenic effects via systemic delivery. Major limitation of GBM chemotherapy is highly selective semipermeable blood brain barrier (BBB) which is comprises of brain microvascular endothelial cells connected by tight junctions. For NSCLC, chemothereutic approaches also have some limitations such as invasive nature and reoccurrence of disease.To enhance bioavailability of drug across BBB and mucus barrier, high dose of drugs is being used which enhances offsite toxicity risk. To reduce offsite toxicity and enhance bioavailability of these drugs biodegradable nanoparticles (<100 nm size) are being developed as carriers to increase high payload of drugs and release drugs in sustained manner thus reduce dose dependent toxicity. Based on previous reports, current project is designed to synthesize and characterize biodegradable nanoparticles for enhanced bio-distribution. First, different biological materials (lipids, Albumin and PLGA), already being used for nanoparticles (NPs) synthesis were optimized and screened to get 100nm sized nanoparticles with high payload of drugs. PLGA and BSA NPs were selected from all due to high payload of drug 5% of PLGA and (9.5%) in BSA compared to other NPs. Both PLGA-PEG and BSA NPs were further characterized to determine morphology and size using zetasizer, TEM. Release kinetics and in vitro anticancer activity of nanoparticles vs free drug was determined against GBM cell lines (F98, (9LL) and NSCLC cell lines (A549) using toxicity assays. Drug loaded NPs showed promising results, released drugs in sustained manner and retained their toxicity. For GBM, BSA formulations were further characterized for in vivo bio distribution in rats and mice brains tissues by convection enhanced delivery (CED) and systemic delivery using fluorescent and confocal microscope. Conventional Polystyrene (PS) and freshly PEGylated PS particles of 40-60nm size were used as standard.Data was analyzed using MATLAB and statistical softwares (GraphPad Prism and Kaleidagraph). Both empty and drug loaded BSA NPs showed highest ex-vivo and in vivo distributions compared to conventional PS-NPs. BSA NPs were successfully synthesized with high payload of both drugs which retained their activity and release drugs in sustained manner. BSA NPs further showed promising in vivo distribution results both locally and systemically compared to conventional particles of same characteristics already available in market.