Muatan Konten Lokal pada Materi Pelajaran Bahasa Inggris di SMP Negeri 5 Sigi

Based on the 2013 curriculum 2016’s revision, there are subject materials that demand about local content in English subjects at the level of SMP Class VII. Teachers need many researches and practices integrating and implementating these subjects. Basically,   it needs to be done in-depth study in the form of content analysis. This research was conducted on content and learning English in SMP Negeri 5 Sigi. This study aims to find out 1) how many percentage of local content in English lesson material Class VII SMP in the curriculum 2013 revision 2016; 2) how to integrate local content in English subject matter in SMP Negeri 5 Sigi; 3) how the implementation of material with local content in English learning in SMP Negeri 5 Sigi, and; 4) how to integrate local content in effective and efficient English lessons in SMP Negeri 5 Sigi. The study used a qualitative descriptive approach with the method of collecting document studies, interviews, and observation. The research results are 1) the proportion of local content that can be integrated into English language learning material SMP is 95%; 2) the integrity of local content in English learning materials in SMP Negeri 5 Sigi can be found in the lesson plan; 3) implementation of materials with local content in English learning attached to KTSP, Syllabus, lesson plan and learning resources enrichment book.

Identification of Gl13 Gene Polymorphism in Polydactyly Patients from Bahawal Nagar District of Pakistan

GLI3 gene is a well-known transcriptional repressor, majorly involved in developing the digits. As it is the promising gene for the digit development so recently many research is in process for exposure of potential application of this gene. The rapid increase of polydactylism in humans has led to more interest on the study of this gene from researcher and scholars. In this study we had taken the blood samples of 50 individuals including different families were collected, age ranges of the patients were from 3-75 years, and had symptoms from moderate to severe from different areas of District Bahawalnagar. Physical sings were used as the selection criteria. 3.5-4ml venous blood was drawn and stored into 50ml tube containing 400?l EDTA, labeled with code number. The results obtained through different characterization e.g. (inorganic method, DNA estimation, sequencing of PCR product and electrophoresis) for GLI3 gene exon 14. Thermo-cycler programs for the amplification of primers were not show any mutation in premier exons. Gel was visualized and in trans-illuminator and hence to ensure the amplified product. No further mutation has been observed in DNA strand. The DNA strands shows similar behavior of GLI3 exon 14 for effected and normal individual. The mutation in this specified GLI3 gene is not responsible for polydactylism. Therefore, GLI3 gene is not accountable for any mutation caused polydactylism in an individual.