میں نے بس تجھ کو چاہا ہے
کون سا ایسا جرم کیا ہے
لوگ مجھے کیوں دیکھ رہے ہیں
مجھ سے کیا کچھ غلط ہوا ہے
تجھ کو جس کی خبر نہیں ہے
تیری یاد میں سوکھ چکا ہے
اُس سے اتنی نفرت کیوں ہے
وہ تو تیرا دوست رہا ہے
تیری جدائی سہوں مَیں کیسے؟
تُو کیوں مجھ سے دور گیا ہے
آج کی رات نہ سو پائوں گا
گلی میں تجھ کو دیکھ لیا ہے
تیری گلی میں جاتا کیوں ہوں
ہوش کہاں مجھ کو رہتا ہے
کوئی تو بات ہے دل میں تیرے
میں نے یہ محسوس کیا ہے
رات بنی ہے سونے کو جی
جانے تُو کیوں جاگ رہا ہے
Literature, among almost all nations, has always been a source of cultural unity & identity. It’s a deep rooted tree quenching the intellectual and cultural thirstiness. Arabic Language, of course deserves to be called a nourishing language-bringing up- for centuries- the nations came up under Islamic caliphate. Almost all world literature is a molded shape of Arabic key sources. Moreover, Arabic language bestowed to look into latent works of Roman. Thus Arabs are the pioneers of presenting quality works-believing in the utilitarian aspect to the world. This language has benignity to collect the then world literature for the purpose of universal awareness. So much more literary works were translated into Arabic with a view to ponder, to discourse and finally to infer. I have presented in this article a glimpse of how the word literature in its pure Arabic context denoted certain contextual definitions since the origin (pre-Islamic period) till the late 4th century A.H.
Pseudomonas aeruginosa is an opportunistic pathogen of humans, animals as well as of plants. It is the most common Gram-negative bacterium found in nosocomial infections. Pseudomonas associated biofilms are highly resistant to high doses of antibiotics. Recent discoveries of quorum quenching (QQ) mechanisms use quorum sensing (QS) as a potential antimicrobial target. Interference with QS can be a novel and effective tool to manage recalcitrant pathogenic microbes. Therefore, the aim of the present study was to monitor the biofilm formation in P. aeruginosa and to examine the efficacy of garlic extract as antipathogenic drugs in selective P. aeruginosa strains. To achieve this goal we investigated the effect of garlic extract and other compounds on P. aeruginosa quorum sensing in terms of biofilm formation. For this purpose, local identified strain (Pseudomonas aeruginosa strain BTP1 (KU534099) and Pseudomonas aeruginosa strain WPP1 (KU534100)) of P. aeruginosa was used. Biofilm formation of P. aeruginosa was determined through tube method, congo red medium and microtitration plate method. Then the N-acyl homserine lactone (N-AHL) autoinducers of P. aeruginosa were detected through biosensors. Garlic (Allium sativum) extract and other selected natural plants extracts like moringa (Moringa oleifera), neem (Azadirachta indica) were tested as quorum sensing inhibitor (QSI) for P. aeruginosa through N-AHL interference assay, pigment inhibition assay, swarming inhibition assay and biofilm inhibition assay. The results outcomes of present study showed that P. aeruginosa infections were found in above-mentioned hospitals with frequency 13.5% (AH), 9% (DHQ) and 7.5% (NH). P. aeruginosa isolates recovered from following sources with a significant rate throat infections (14.7%), wounds (13.3%), hospital instruments (8.6%) and soil (3.3%). Pyocyanin production, swarming and biofilm could be reduced up to 55-60%, 65-75% and 75-85% by ethanolic extracts of garlic, neem and moringa. Through these studies, we explored the potential of these compounds as antipathogenic drugs.