ڈاکٹر عبدالمعید خان
جامعہ عثمانیہ حیدرآباد کے شعبہ عربی کے صدر ڈاکٹر عبدالمعید خاں کی وفات علمی حلقہ کے لیے ایک سانحہ ہے، انھوں نے قاہرہ اور کیمبرج میں تعلیم پاکر ساری عمر جامعہ عثمانیہ کی خدمت میں گذاری، کچھ دنوں آکسفورڈ یونیورسٹی میں بھی عربی کے پروفیسر رہے، حیدر آباد کے مشہور انگریزی رسالہ اسلامک کلچر کی ادارت کے فرائض آخر وقت تک بڑی خوبی سے انجام دیئے مارماڈیوک پکتھال نے اس کا جو معیار قائم کیا تھا، اس کو انھوں نے قائم رکھا، دائرۃالمعارف حیدرآباد کی علمی سرگرمیوں میں بھی ان کا بڑا حصہ رہا، ان کی رہنمائی میں یہاں سے بہت سی مفید کتابیں شائع ہوئیں، مولانا ابوالکلام آزاد ان کی علمی صلاحیتوں کے معترف تھے، وہ حکومت کی علمی کمیٹیوں میں نامزد ہوتے رہے، جہاں وہ عزت کی نظر سے دیکھے جاتے تھے، امید ہے کہ جامعہ عثمانیہ ان کو ایک نامور فرزند کی حیثیت سے برابر یاد رکھے گی۔
(صباح الدین عبدالرحمن، نومبر ۱۹۷۳ء)
Ijmāʿ is an important mode of Ijtihād and well known principle of Islamic Sharʿiah. Historically it is evident that incidence of Ijmāʿ )Consensus( restricted only to four Caliphates of Islam only. This Collective Ijtihād and Collective Opinion was actually the decision of the Islamic State followed and obeyed by the all Muslims specially by "Ṣaḥābah" (Companions of the Holy Prophet), this is why it is called Ijmāʿ-e- Ṣaḥābah. These decisions were applicable and binding to all Muslims living elsewhere in the world, because at that time there was centralized ruling system (Khilāfat-e-Wāḥidah). Now Muslim world has split into many states, so every state has its own decision making institutions and hence such Ijtihād and Ijmāʿ, Islamic Legislation Activities should be validated within those states as Ijtihād and Ijmāʿ except issues relevant to general interest as whole human being and all Muslims. In such issues International level consensus of Islamic Jurists would be required. "Ijmāʿ", actually it is the same processes. More over any "Ijmāʿ" held in a time period can be revoked by any new situation in future as per requirement of the time. It is the inevitable demand of dynamism of Islam to correlate it to every need of the time.
Escherichia coli lineage ST131 predominates across various spectra of extra-intestinal infections. Distinctive resistance profile, diverse armamentarium of virulence factors and rapid global dissemination of ST131 E. coli makes it an intriguing pathogen of significant importance. However, little is known about the prevalence and various attributes of ST131 associated with urinary tract infections in Pakistan. Evolutionary dimensions of ST131 linage and corresponding molecular markers encoding drug resistance remained largely unexplored and prevalence data are extremely scarce in South Asian countries. We scrutinized prevalence and various genetic attributes of E. coli ST131 isolates involved in urinary tract infections by processing 148 randomly selected samples during August 2012 and August 2014. Phylogenetic grouping, fumC/fimH and O-typing was done by PCR based methods. Antibiotic susceptibility assays and phenotypic detection of ESBLs were carried out according to the CLSI guidelines of year 2013 and ESBL isolates were screened for the prevalence of blaCTX-M-15. Plasmid encoded qnrA, qnrB and qnrS genes were identified by DNA amplification and sequencing. Mutations in genes gyrA, gyrB, parC and parE were identified among fluoroquinolone resistant isolates. Minimum inhibitory concentrations (MICs) for ciprofloxacin and levofloxacin were determined by using broth dilution method. Phylogenetic analysis was performed by PCR using three gene classification systems (Clermont et al., 2000). Overall, 59% of the UPEC isolates belonged to phylogenetic group B2 which was followed by group D=28%, B1=8% and A=5%. Among 18 different ST-types ST131 was the most dominant lineage as 46% of the isolates belonged to ST 131 lineage. By, CH-typing 46% of the ST 131 isolates belonged to sub-group H30. Higher numbers of identified ST 131 isolates 74% were MDR and 44% showed ESBL phenotypes while 100% of the isolates carried blaCTX-M-15. Overall, resistant patterns of ST 131 isolates confirmed least resistance against tigecycline, meropenam and amikacin. Likewise, resistance against tazocine, nitrofurantoin, sulzone and minocycline remained below threshold level. For fosfomycin, (FF) and gentamicin (CN) resistance were 13% and 28% respectively. Higher resistances was noticed against all four generations of cephalosporins, (53%-78%). Higher percentages of ST 131 isolates were resistant to co-trimoxazole (85%), amoxicillin clavulanic acid 65% and fluoroquinolones 60%. Overall, minimum inhibitory concentrations of ciprofloxacin remained above 256μg/ml in ST 131 and non ST 131 isolates. In comparison to MICs of ciprofloxacin, MICs of levofloxacin in most of the isolates varied between 8μg/ml-64μg/ml. In this study, majority of the fluoroquinolone resistant UPEC ST 131 isolates carried at least two or more non-synonymous mutations in gyrA, parC and parE genes. These isolates showed higher MICs for both ciprofloxacin and levofloxacin. Conclusively, higher percentages of the ST 131 isolates showed antibiotic resistance against trimethoprimsulphamethoxazolen, cephalosporin, fluoroquinolone, and majority of these isolates were multi-drug resistant. In addition, fluoroquinolone resistant UPECs isolates shared lineage of a clonal sub-group. An allelic combination of gyrA/parC/parE genes of sub-clone O25b-ST131 H30-R was associated with higher MICs of ciprofloxacin and levofloxacin. Overall, it is the first report from Pakistan that provides details insights about susceptibility patterns and genetic attributes of pandemic MDR ST131 strains involved in UTIs.