مارک، ایڈورڈ ویسٹر
ایڈورڈ ویسٹر مارک کا انتقال
ایڈورڈ ویسٹر مارک نے جو ایک عرصہ تک لندن یونیورسٹی میں عمرانیات کا پروفیسر تھا، گزشتہ اکتوبر میں انتقال کیا، وہ ایک عمیق النظر فلسفی اور بین الاقوامی شہرت کا مالک تھا، اوائل زندگی میں اس کو خیال پیدا ہوا کہ اخلاقی خیالات اور مذہبی اعتقادات میں گہرا تعلق ہے، چنانچہ اس نقطہ نظر سے اس نے یورپ کے علاوہ دوسری قوموں کے روایات اور اعتقادات کا مطالعہ کرنا چاہا اور اس کے لئے مراکش کو منتخب کیا، اور وہاں جاکر چار سال تک مقیم رہا، یہاں اس نے نہ صرف انسانیاتی معلومات جمع کئے، بلکہ وہاں کے لوگوں طرز زندگی و غور و فکر سے بھی واقفیت حاصل کرکے ان کے رسم و رواج کو آسانی سے سمجھا جو تمدن کے مختلف دور میں پیدا ہوتے رہے، یہاں کی تحقیقات اس نے اپنی مشہور کتاب ’’اخلاقی تخیلات کی ابتدا اور نشوونما‘‘ (The origin and development of moral ideas) میں قلمبند کی جو دو جلدوں میں ۱۹۰۶ء اور ۱۹۰۸ء میں شائع ہوئی تھی، یہ کتاب بہت اہم اور بلند پایہ سمجھی جاتی ہے اور اخلاقیات پر ایک فلسفیانہ مقالہ یا اخلاقی خیالات کی تاریخ کہی جاسکتی ہے، اس کتاب کی خوبی یہ ہے کہ اس میں تحلیلی اور تاریخی تجزیوں میں امتزاج پیدا کرکے یہ دکھایا گیا ہے کہ اخلاقی پسندیدگی اور ناپسندیدگی کی ابتدا معاشرتی تکدر اور تشکر سے ہوتی ہے، ڈاکٹر ویسٹر مارک کی اور دوسری تصنیفات یہ ہیں، مراکش میں انسانی شادی، رسم اور اعتقاد کی تاریخ، (۲)اخلاقی اضافیت، (۳)عیسائی مذہب اور اخلاق۔
(’’ص ۔ ع‘‘، دسمبر ۱۹۳۹ء)
The purpose of this paper is to guide about the main reason of clashes between revealed religions in the society. We do not clarify our vision regarding religion. People do not know about the basic ethics of our religions. So, it creates moral illness in the society. We have narrow approaches about religion and took it in very conservative thoughts. For getting out of extreme level of destruction and moral degradation it is necessary to build a universal society which consists of those social values which are common in all religions. People are inclined towards ills and far away from God that is why our society is a victim of destruction. These ills made them to go far away from God and religion. In the present era the situation is the same, people do not understand the religion properly. Only through this proper understanding, destruction, prejudice, extremism and cruelty can be removed from the society. All the religions have some common features as justice, honesty, courtesy, patience etc these features are the ethical as well as religious codes of a society and if all people follow these features an ideal society can be established. This study covers revealed religions all around the world.
Codon optimized synthetic epsps gene was used to develop transgenic tobacco and sugarcane lines using Agrobacterium and biolistic approach, respectively. Putative transgenic tobacco lines analyzed by qRT-PCR showed variable mRNA transcript levels. Southern hybridization performed for determining gene integration patterns showed multiple copy inserts (1-4) of transgene in various tested lines. Immunoblot strips confirmed the translation of cp4-epsps mRNA transcripts in T1 line. Malachite green assay showed variable EPSPS activity levels in various lines in response to glyphosate application. Transgenic tobacco lines exposed to 1% (v/v) glyphosate at 4 to 5 leaf stage showed variable resistance levels ranging from moderate level tolerance to high tolerance. The cp4-epsps mRNA transcript levels varied from genotype to genotype in tested transgenic sugarcane lines regardless of the integration of multiple copy number (3-6) of transgene. Genetic transformation with pyramided constructs results in the integration of multiple genes at a single locus, which is desirable in terms of expression efficiency of transgenes. The cp4-epsps gene was pyramided with insect resistant cry1Ac and cry2Ab genes and cloned in pSb187 vector was used to genetically transform tobacco using Agrobacterium mediated approach. Transgenic lines were confirmed through PCR using gene specific primers of respective genes. The mRNA transcript level of cp4-epsps gene detected through qRT-PCR using GAPDH gene as an internal control was found to be variable among different transgenic lines. Transgenic lines were tested for checking the translational efficiency of all three genes using immunoblot strips. Transgenic tobacco lines found expressing all three proteins were subjected to detached leaf insect bioassays to evaluate the effectiveness of Bt genes in putative transgenic tobacco against insect attack using first instar larvae from Spodoptera littura and Helicoverpa armigera. Transgenic tobacco found to be resistant against these insects showed that cry1Ac and cry2Ab genes were efficient enough in pyramided form. When these transgenic tobacco lines were exposed to glyphosate at 10 to 12 leaf stage, chlorotic symptoms were observed which hinted that herbicide tolerance potential of transgene declined with increasing age of the plants. In order to overcome the spatio temporal gene expression issues, a dual copy epsps expression cassette synthesized under different regulatory elements, cloned in pGreen0029 binary vector was used to generate transgenic tobacco. These tobacco lines exhibited excellent resistance against 19% (v/v) glyphosate at 10-12 leaf stage. This construct has shown tremendous potential regarding transgene expression levels which is required in the field crops. This dual cassette cp4-epsps gene has been recommended for generation of glyphosate resistant cotton and other commercially important cultivars.