منقبت درِ شانِ حضرت علی کرم اللہ وجہٗ
مخزنِ صدق و صفا مولا علیؓ
پیکرِ شرم و حیا مولا علیؓ
منبعِ جود و سخا مولا علیؓ
مرکز مہر و وفا مولا علیؓ
بے نوائوں کی نوا مولا علیؓ
بے ردائوں کی ردا مولا علیؓ
میری ہر تسکین کا باعث ہیں وہ
میرے ہر دُکھ کی دوا مولا علیؓ
میرے سر پہ سایۂ حسنین ہے
میں ترے در کا گدا، مولا علیؓ
مجھ سے تائب مشکلیں رہتی ہیں دور
میرے ہیں مشکل کشا مولا علیؓ
Quran has deep relation with science. For Example Quran teaches us about the creation of the world, human being, all the living and nonliving things and its advantages and disadvantages. Quran also tells us about the different diseases and its cure. Now in the modern time the scientist are following the Quran for their scientific research. There are 13 hundred verses in Quran belongs to human thoughts. Just like this there are different explanation about the human’s health and food and these things are related to science. The Quran has presented knowledge about mountains, sea, water, air environment etc.
Oral squamous cell carcinoma (OSCC) is the most common malignancy in head and neck region with an occurrence rate of 500,000 per year. Higher incidence and mortality rates have been observed in south-east Asia including Pakistan. The common sub-site found in Pakistani population and other physiological variations are strongly linked with the increasing use of smokeless tobaccos (SLTs). Early diagnosis plays a key role in treatment and monitoring of any cancer. Histopathology is the only diagnostic method recommended for OSCC which is not very effective for early stage diagnosis. Thus, biomarker studies have been conducted all around the world using proteomics techniques in search of potential candidate diagnostic biomarkers. In current study, we have performed comparative proteomic analysis using buccal mucosa OSCC tissue samples and their adjacent normal mucosa. The samples were subjected to two-dimensional gel electrophoresis and differentially expressed spots were selected for Mass spectrometric analysis. We have identified seventeen proteins that were consistently differentially expressed among most of the samples analyzed. The differentially expressed proteins that have been uniquely identified in this study include Serum albumin (ALBU), Ig Kappa chain C region (IGKC), and Isoform 2 of fructose bisphosphate aldolase A (ALDOA). We suggest that further research would be required to investigate their role in carcinogenesis and whether any of these could serve as potential biomarker. The significantly altered expression of Calreticulin (CALR), Heat shock protein beta 1 (HSPB1), Heat shock protein 70 (HSC70), Cytokeratin-8 (KRT8), Cytokeratin-16 (KRT16), Cytokeratin-14 (KRT14), Cytokeratin-17 (KRT17), and 14-3-3σ/Stratifin (SFN) found in our study strongly supported the earlier reported results. We also performed immunohistochemistry (IHC) to confirm the higher expression of SFN as potential biomarker. SFN plays an important role in p53 apoptotic regulation and therefore findings of our study reveal and suggest it as a potential candidate biomarker in Pakistani population. XII Considering the association of oxidative stress with cancer progression, we also studied markers of oxidative stress in OSCC including Paraoxonase1 (PON1), Reactive oxygen species (ROS), and Malondialdehyde (MDA). We found significant relationship among oxidative stress indicators, particularly PON1 with OSCC suggesting PON1 as predictive marker.