عہد نبوی میں ذرائع کے اسالیب عصر حاضر کے نوجوانوں کے لیے مشعل راہ

Since from the beginning of humanity means of communication have always been an essential need for mankind. To convey the message and to find means to communicate and express one’s thought one needs a mean to transmit the information to others. That is called communication. With the passage of time and advancement communication means also took modern shape and became advance. Islam as a complete code of life, guides humanity in the all fields of sociology, economic, politics, including mass communication. Allah the Almighty sent messengers and Prophets for the guidance of people. So they served the humanity in different periods of time in different areas. Ḥaẓrat Muḥammad (ﷺ) the last prophet of Allah used these means of communication for the prevalence and preaching of Islam, and left behind a remarkable legacy in the field of mass communication for the guidance till dooms day. Where there have been great changes in other fields and professions of life in the advanced world of contemporary era there had become a revolutionary change in the field of media. News all over the world spread in seconds. Media which is the strongest tool to approach people, but sorry to say that it is detracted badly by prevailing wrong values, vulgarity, jealousy and selfishness. There is a dire need to change the direction of noble that so (صلى الله عليه وسلم) Muhammad Prophet of methodology the towards media values like piousness, self-sacrifices, brotherhood and cooperation should be developed in people and for this purpose youth can play a pivotal and effective role in the field of mass communication. Eyes are looking towards youth of contemporary era to step forward by following our Holy contemporary the how that attempt humble a is article This. (صلى الله عليه وسلم) Prophet youth can play its role by using means of communication by taking guidance from Prophetic Era to lead media towards right direction.

Effect of 5 Deletions of Efficiency and Specificity of Osrglp2 Promoter

Germin like proteins (GLPs) are member of a large gene family and ubiquitously expressed as plant proteins. The exact mode of action and role in metabolism is not well understood. It is believed that these putative stress proteins are expressed at various developmental stages in response to abiotic and biotic stresses. The present study was designed to clone full length and 5'' abbreviated Oryza sativa Root expressed GLP2 (OsRGLP2) promoter, its genetic transformation into Arabidopsis and characterization. Cloning of full length and 5'' abbreviated OsRGLP2 promoters was carried out by employing GATEWAY™ Technology. Amplification was performed by specific primers designed on the promoter region. Recombinant entry clones were created by using pENTR/D-TOPO® cloning kit. Expression vectors were prepared by employing LR recombination reaction between recombinant entry clones and promoter less destination vector pHGWFS7. Confirmation was carried out by PCR and sequencing. Recombinant expression vectors were named as pNSS-F1 (Full length promoter) and pNSS-F2, pNSS-F3 and pNSS-F4 (5'' deleted promoters). Plant transformation into Arabidopsis was carried out by Agrobacterium mediated floral dip method. T0 and T1 lines were established and transgenic plants were analyzed by molecular and physiological approaches. T1 transgenic lines for each promoter constructs were selected through GUS assay and tested for wound, salt and temperature stresses. Real-time PCR was performed by using two sets of primers, Actin (Housekeeping gene) and GFP (Gene of Interest). Real-time PCR analysis was done by employing 2-ΔΔCt method in which data was normalized by software inbuilt in the real-time PCR system by taking calibrator or untreated sample value xxi as 1. The graphical data shows the times fold increase or decrease in the expression with comparison with untreated control samples. Expression analysis revealed that OsRGLP2 promoter is efficient and specific to wound, salt and temperature stresses. When comparison was done between full length and 5'' deleted promoters, the promoter named pNSS-F3 of 565 bp along with other two larger promoter pNSS-F1 and pNSS-F2 of sizes 1063 bp and 776 bp respectively were responding to all stresses applied during this study. However, when a further deletion was made and shortest promoter pNSS-F4 was created which comprises of 283 bp size, unable to respond against stresses applied. So it can be concluded from present study that during 5'' deletion of full length OsRGLP2 promoter (creation of pNSS-F4), a critical region between P-565 and P-283 on promoter fragment was deleted which contain promoter elements necessary to respond against certain stresses like wound stress, salt stress and temperature stress. While during other deletions (in case of pNSS-F2 and pNSS-F3) in which when the critical part was intact, the promoter responded to applied stresses. It can be further concluded that pNSS-F3 is a good replacement for full length promoter. Due to smaller size of pNSS-F3 promoter (565 bp) and it‟s efficient and specific response against abiotic stresses it can be fused with other promoter and used in combination against certain abiotic or biotic stresses.