دنیا اس وقت گلوبل وِلج بن چکی ہے۔ ایک دوسرے سے آزادانہ تجارت ،دفاع،تعلیم ، ٹیکنالوجی ، توانائی اور ماہرین کے تبادلوں کے معاہدے ہو رہے ہیں۔کوئی بھی ملک معاشرتی ، معاشی، تجارتی اور سیاسی طور پر ایک دوسرے کے رابطے اور تعاون کے بغیر نہیں چل سکتا اور یہ وقت کی ضرورت بھی ہے کہ ایک دوسرے کے ساتھ اچھے تعلقات رکھے جائیں ۔ دفاع ، توانائی ، تعلیم ، جدیدٹیکنالوجی ، تجارت، ماہرین اور قرضوں کی وجہ سےمسلمان ممالک عالمی برادری کے ساتھ تعاون پر مجبور ہیں حالانکہ سب سے زیادہ وسائل مسلمانوں کے پاس ہیں لیکن ان وسائل کوصحیح طورپر استعمال نہ کرنے اور آپس میں اتفاق واتحاد نہ ہونے اورصحیح منصوبہ بندی نہ کرنے کی وجہ سے عالمی ادارے اور عالمی برادری مسلمانوں سے اپنی مرضی کے مطابق کام کرواتی ہے، پا لیسیاں تبدیل کرواتی ہے اور ان کی ہدایات پر عمل کرنا ہماری حکومتوں ومقتدرشخصیات کی مجبوری ہے۔ حدود و قصاص سے متعلق قانون سازی اور ان قوانین پر عمل درآمد کے سلسلے میں مسلم دنیا کو بین الاقوامی طور پرعالمی برادری کے رویوں کی وجہ سے کافی مشکلات کا سامنا ہے ۔ یہ مشکلات اور مسائل مندرجہ ذیل ہیں:
Allah has created human being and gave him the power of intellectual understandings. To utilize and make positive use of this Allah sent Holy books for guidance. While, keeping the sequence of these books and the historical evolution of human mind, one can easily recognize the symmetrical progress in both. Human starts living in caves and through his intelligence created homes to live, tools to gain meal, and so on, till todays growth. As far as, evolution of Holy books is considered, Scriptures were revealed in different portions as human at that stage couldn’t write or store on pages or another source. Old Testament was revealed earlier. It is combination of Torah, Psalms and other books. In the context of Islam, Torah was the first book revealed on Hazrat Mosa, in which commandments were given as per the level of Bani Israel. Afterwards, Psalms, containing poetic way inspires Bani Israel after the Torah and the Bible Last Holy book in the generation of Israel, comprises of observational and experiential way, as required in that era. Holy Quran, compiled and final version of all the past version, is summary and elemental book. In which, descriptive, observational, experiential, poetic, logical, argumentative and comprehensive technique are present. These all shows, ultimate intellectual growth of human is being address in Holy Quran. As Human is now intellectually at its peak so the teaching and techniques used are at highest level of literature, showing the step by step evolution and association of Holy books with Human being.
The object of this study was to synthesize highly biocompatible and stable gold, silver and copper nanoparticles, without use of toxic reducing and stabilizing agents in aqueous media. In order to achieve this objective, natural polysaccharides (hemicelluloses) were isolated from Plantago ovata (PO), Mimosa pudica (MP) and Lallemantia royleana (LR) and used as reducing and stabilizing agents. The isolated polysaccharides were characterized by elemental, moisture, optical rotation, FTIR and MALDI-ToF analyses. They were found to be highly branched hemicelluloses. It was discovered that the hemicelluloses can effectively reduce noble metal ions due to presence of aldehyde groups in their structures. These hemicelluloses can absorb water several times their mass and swell like hydrogels. In swollen form they can encapsulate and disperse nanoparticles in their matrix, as evidenced by electron microscopy. The dispersion was effective in keeping the particles from each other over longer periods of time. Metal nanoparticles were readily obtained after mixing the metal salts with suspensions of these hemicelluloses in water. The reaction rate followed the order: PO ≥ LR > MP. The reduction process was witnessed by colour change (from yellow to blue/purple/ruby red depending upon the pH and reaction temperature) and monitored by recording the surface plasmon resonance spectra in the 350-800 nm range. The synthesized nanoparticles exhibited spherical shapes. Size of particles was found to depend on amount of hemicellulose used, pH and temperature of reaction mixture. Effect of these parameters was optimized by response surface methodology to get smallest possible size of the particles. The lowest surface plasmon resonance peaks representing smallest size obtained by reduction of gold with MP, PO and LR were 530, 520 and 517 nm, respectively. The SPR spectra of AgNPs started building up at pH > 6 and exhibit maxima at 412, 396 and 387 nm with PO, MP and LR, respectively. The smallest particle sizes obtained under optimum conditions were ~ 10 nm (with LR), ~10 nm (with LR) and 70 nm (with LR), respectively of gold, silver and copper. The synthesized gold nanoparticles were found to be non-toxic as shown by cytotoxic tests. The size of the gold nanoparticles ( > 30) obtained by use of MP falls in the range that is suitable for cellular uptake in various cancer cell lines, as the gold nanoparticles having size > 30 nm can rarely enter nucleus of the cell, so are suitable for safe drug delivery. On the other hand nanoparticles of silver and copper, exhibited dose-dependent antimicrobial activities against E. coli, A.tumefaciens, P. aeruginosa, B. subtilis and S. aureusand, they were found to be cytotoxic against brine shrimps and Hela cell lines and the tumours caused by A. tumefaciens. These results suggest that gold nanoparticles can be used for safe drug delivery without any therapeutic effect of their own, whereas nanoparticles of silver and copper can be used as anti-infective and anti-cancer agents. Gold nanoparticles being non-toxic were subjected to bio-distribution studies in rabbit and tumour bearing mice by use of radio labelled gold (198Au) nanoparticles. The uptakes differed with the use of hemicelluloses. Highest uptake of gold nanoparticles (radiolabelled) dispersed in PO mucilage, after 3h, was found in spleen followed by liver, heart, muscle, kidneys and lungs in rabbit. In case of gold nanoparticles synthesized by use of MP the highest uptake was in spleen followed by liver, kidneys, blood and bladder after 3h. They depleted with time in spleen and were significantly higher in liver after 24h. The particles also accumulated in epithelium carcinoma tumour. After oral administration of radiolabeled gold nanoparticles to rabbit, it was found that the particles were released to colon due to solubility of hemicelluloses in basic media suggesting that they can be used for targeted delivery at colons. In serum proteins the gold nanoparticles bound preferentially to globulins with the order; α1-globulin (6.5%) followed by α2-globulin (~2%), β-globulin (~1%), γ-globulin (0.707%) and albumin (0.66%).