4۔تحفظ نسل
شریعت مطہرہ نے عفت و عصمت اور انساب کی حفاظت کی حفاظت پر بہت زور دیا ہے اور اس کے تحفظ کے لیے از حد اقدامات فرمائے۔ مختلف قبائل میں مختلف خصوصیات پائی جاتی ہیں اور انہی خصوصیات کی بنا پر اس خاندان یا قبیلہ کا تعارف ہوتا ہے ۔ نسل کے تحفظ میں مختلف خصائص کے علاوہ تقسیم وراثت میں بھی آسانی کا پہلو شامل ہے اسی وجہ سے اپنی ذاتی نسبت غیر خاندان کی جانب کرنے پر شریعت میں سخت وعید سنائی گئی ہے اورجہاں زنا کو حرام قرار دیا وہاں زنا تک پہنچنے سے پہلے اس گناہ کے دروازے ہی بند کردیے نظروں کو نیچا رکھو، مخلوط محفل پر پابندی ، عورت کی عزت کو خانہ کعبہ سے افضل قرار دیا ، پردے کا حکم جاری کیا، قوت رجولیت میں کمی کے لئے روزوں کا حکم ، جذبہ حیا کا فروغ ، نکاح کو آسان بنانا اور پھر اگر انصاف کر سکتا ہو اور طاقت رکھنے کی صورت میں چار شادیوں کی اجازت دی۔
نگاہوں کو نیچا رکھنے کا حکم
﴿قُلْ لِلْمُؤْمِنِينَ يَغُضُّوا مِنْ أَبْصَارِهِمْ وَيَحْفَظُوا فُرُوجَهُمْ ذَلِكَ أَزْكَى لَهُمْ إِنَّ اللَّهَ خَبِيرٌ بِمَا يَصْنَعُونَ ۔ وَقُلْ لِلْمُؤْمِنَاتِ يَغْضُضْنَ مِنْ أَبْصَارِهِنَّ وَيَحْفَظْنَ فُرُوجَهُنَّ ۔ ﴾241
"مؤمن مردوں سے کہ دو کہ اپنی نظریں نیچی رکھا کریں اور اپنی شرم گاہوں کی حفاظت کیا کریں یہ ان کے لیے بڑی پاکیزگی کی بات ہے بے شک اللہ اس سے باخبر ہے جو کام یہ لوگ کرتے ہیں۔ اور مؤمن عورتوں سے بھی کہ دو کہ وہ بھی اپنی نگاہیں نیچی رکھا کریں اور اپنی شرمگاہوں کی حفاظت کیا کریں ۔ "
روزے رکھنے کا حکم
جناب علقمہ بن قیس (م:62ھ)سے مروی ہے کہ رسول اللہ ﷺ نے فرمایا:اے نوجوانو!
"من استطاع منكم الباءة فليتزوج فإنه أغض للبصر وأحصن للفرج ومن...
Since the “text” has received attention of all modern and post modern scholars and linguists, it has been changed. This “text” has different segments, some of them are applicable to Holy Qur’ān on the one hand, on the other hand there are some un-applicable too in connection of two textual boundaries of the “text”; bounded and un-bounded. From here many scholars declare that modern defined textual theory is totally un-applicable to Holy Qur’ān. Therefore, many Qur’ānic commentators and scholars refused “Intertextuality” with its all aspects. Anyhow some other scholars allow “Intertextuality” to be applied to Holy Qur’ān because of many Qur’ānic scholars have been interpreting this sacred text using the structural methods upon which contextual and intertextual phenomena and all the relevant conceptual components of this are based, this research intends elaboration of such dimensions of Qur’ānic “Intertextulaity” and “Textuality”, in particular, the Qur’ānic textulaity; “al-Nasḵ”; abolition, deletion and abrogation.
Archaea represent the third domain of life and comprise a highly diverse class of microorganisms some of which can withstand extremes of temperature, pressure, pH and salinity. It is for this reason that members of this group are also collectively referred to as ―extremophiles‖. Archaea have a number of unique features such as ether-linked lipids in their cell membranes but also share several important characteristics with eukaryotes and bacteria. For example, like bacteria archaeal genomes are circular but their gene promoters and transcriptional apparatus is more closely related to the eukaryotic RNA polymerase-II system. In Sulfolobus, a model crenarchaeote, transcription is dependent on TATA binding protein (TBP), transcription factor-B1 (TFB1), and perhaps also on transcription factor-E (TFE) which serve as specificity factors for the 12 subunit RNA polymerase. Sulfolobus genome also encodes for other putative transcription factors such as TFB2, and TBP- interacting protein-49 (TIP49) whose functions remain elusive. All cells are capable of coping with transient thermal and chemical stresses by triggering expression of discrete sets of genes whose products prevent cell death. Such responses have been well documented in bacteria and eukaryotes but the effect(s) of such insults on cell morphology, proteome, genome, transcription as well as on the fates of various components of transcription in archaea remain unknown. In this study it was hypothesised that stress modulates the expression and/or stability of one or more components of Sulfolobus transcriptional apparatus. To test this, the cellular and biochemical consequences of subjecting Sulfolobus solfataricus P2 to chemical and thermal stresses as well as their effects on Sulfolobus transcription machinery were studied. Results show that elevating the temperature from 76 ̊C to iv90 ̊C (heat shock) for 5 minutes results in large scale protein aggregation and altered cellular morphology of Sulfolobus heat shocked cells. Moreover, immunochemical analyses suggested that TFE levels in heat shocked cells experience a rapid decline while its mRNA levels continue to rise even after 30 minutes of heat shock. Furthermore, temperature-shift experiments demonstrate that outgrowth of heat shocked cells is dependent on restoration of TFE levels. While heat shock promotes selective depletion of TFE and does not affect genomic or proteomic integrity to any significant extent, exposure of cells to >0.25% isopropanol or >100 mM hydrogen peroxide is detrimental. Specifically, cells treated with 2% isopropanol or 200mM hydrogen peroxide alter their morphologies and harbour degraded genomes as well as proteomes that are partially depleted. Isopropanol and hydrogen peroxide exposure does not promote random protein degradation but instead preferentially impacts fates of certain transcription factors. Whereas isopropanol mediated degradation of genomic DNA in Sulfolobus cells is not affected by EDTA, oxidative stress-induced genomic breakdown is inhibited with EDTA. Moreover, the damaging effects of 2% isopropanol or 200 mM H 2 O 2 on host genome and proteome are restricted to Sulfolobus and are not observed in either bacterial or cultured eukaryotic human cells. Taken together, these results demonstrate that in Sulfolobus solfataricus P2 cells: 1) TFE is depleted by heat shock and does not appear to function as a general transcription factor, 2) thermal and chemical stresses impact the stability of TBP, TFB1, TFE, TIP49 and RpoB differentially, and 3) isopropanol and hydrogen peroxide mediated genomic DNA degradation is observed only in archaeal cells and likely occurs through different mechanisms.