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Home > Kafalat Aamah Ke Raij Models Aur Hayat Tayyabh Wa Sahabah Se Rahnumaeeki Sortein [M. Phil Islami Fikro Tahzeeb] = کفالت عامہ کے رائج ماڈلز اور حیات طیبہ و صحابہ سے رہنمائی کی صورتیں

Kafalat Aamah Ke Raij Models Aur Hayat Tayyabh Wa Sahabah Se Rahnumaeeki Sortein [M. Phil Islami Fikro Tahzeeb] = کفالت عامہ کے رائج ماڈلز اور حیات طیبہ و صحابہ سے رہنمائی کی صورتیں

Thesis Info

Author

Sohaib Sarfraz =صہیب سرفراز

Department

Umt. School of Social Sciences and Humanities. Department Islami Fikro Tahzeeb

Program

Mphil

Institute

University of Management and Technology

Institute Type

Private

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Page

148 . CD

Subject

Islam

Language

English

Other

School of Social Sciences and Humanities; Urdu; Call No: TP 297.272 SOH-K

Added

2021-02-17 19:49:13

Modified

2023-02-19 12:33:56

ARI ID

1676714223066

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مولانا شہاب الدین ندوی

مولانا شہاب الدین ندوی
قارئین معارف کو اس اطلاع سے نہایت رنج ہوگا کہ ان کے محبوب اور معارف کے خاص مضمون نگار مولانا شہاب الدین ندوی ۱۹؍ اپریل ۲۰۰۲؁ء کو سفرِ آخرت پر روانہ ہوگئے، اناﷲ وانا الیہ راجعون۔
وہ کئی برس سے موذی امراض میں مبتلا تھے اور چند ماہ سے موت و حیات کی کشمکش میں گرفتار تھے جس کی تفصیل مجھے ۳۱؍ دسمبر ۲۰۰۱؁ء کو اپنے والا نامہ میں اس طرح لکھی تھی۔
’’طبیعت بہت زیادہ خراب اور نڈھال رہتی ہے، کئی کئی دن بستر پر پڑا رہتا ہوں، علاج و معالجے سے کوئی فائدہ نہیں ہورہا ہے کئی قسم کے امراض میں مبتلا ہوں اور ڈاکٹر ان کی تشخیص نہیں کرپارہے ہیں، ایک بیماری ذرا دبتی ہے تو دوسری ابھر کر سامنے آجاتی ہے ان جان لیوا بیماریوں سے تنگ آچکا ہوں اور مزید طرفہ یہ کہ اب آنکھوں کی بینائی بھی مسلسل ضائع ہوتی جارہی ہے۔ داہنی آنکھ کا آپریشن ہوا مگر بینائی بڑھنے کے بجائے گھٹ رہی ہے، معلوم ہوتا ہے کہ اب میں صرف چند دن کا مہمان ہوں لیکن طبیعت جب کبھی ذرا سنبھلتی ہے تو جم کر لکھنے اور اپنے مسودات صاف کرنے بیٹھ جاتا ہوں‘‘۔
آخر وہی ہوا، خط لکھنے کے ساڑھے تین مہینے بعد وقت موعود آگیا اور مولانا کی ساری دینی، علمی اور تحقیقی سرگرمیاں ہمیشہ کے لیے بند ہوگئیں۔
مولانا کا وطن بنگلور تھا۔ میٹرک کی تعلیم یہیں ہوئی اور دینی تعلیم کی تکمیل دارالعلوم ندوۃالعلماء لکھنؤ میں کی۔ طالب علمی ہی کے زمانے سے ان کی طبیعت کا رجحان قرآنی علوم اور سائنس کی جانب ہوگیا تھا اور وہ ان میں تطبیق اور ہم آہنگی پیدا کرنا چاہتے تھے اسی لیے لکھنؤ کے بعض کالجوں کے سائنس کے اساتذہ سے ان کا ربط و ضبط ہوگیا تھا، دارالعلوم سے فراغت...

Majmu’ Hadits Integrasi Ilmu dan Islamisasi Digital Majmu' Hadith Integration of Science and Digital Islamization

Sains (ilmu pengetahuan) merupakan sesuatu yang penting dalam agama Islam. Integrasi sains dan agama berarti berupaya untuk memadukan antara sains dan agama, tak harus berarti menyatukan atau bahkan mencampuradukan, karena identitas atau watak dari masing-masing kedua entitas itu tak mesti hilang dan harus tetap dipertahankan. Dalam paradigma Islam, integrasi antara agama dan sains adalah sesuatu yang mungkin adanya, karena didasarkan pada gagasan ke-Esa-an (tauhid). Sudah saatnya, sains dan agama harus menghadirkan kesadaran yang muncul lewat pandangan-pandangan yang lebih harmonis, holistik, dan komprehensif. Alat digital merupakan salah satu medium terbaik untuk tujuan tersebut. Artikel ini merangkum bagaimana Integrasi Ilmu dan Islamisasi Digital menciptakan ruang bagi umat Islam untuk menggabungkan spiritualitas dengan teknologi, menghasilkan dampak positif dalam berbagai aspek kehidupan. Dengan mengambil langkah-langkah menuju integrasi ini, umat Islam dapat menjaga identitas keagamaan mereka sambil tetap berkontribusi dalam era digital yang terus berkembang.

Molecular Studies for Regulation and over Expression of Tylosin from Streptomyces Fradiae Nrrl-2702

Tylosin is a macrolide antibiotic used as veterinary drug and growth promoter. Attempts were made for over expression of tylosin through random mutagenesis of Streptomyces fradiae NRRL-2702 using ultraviolet (UV) and gamma (γ) irradiations. After 100 and 120 seconds exposure of UV (λ=300 nm), spore suspension of wild type strain exhibited 5% and 8% survival rate, respectively and these exposure times were found suitable to isolate the most potent mutant colonies. 300 colonies appeared on the agar plates at above mentioned exposure times including six morphologically altered colony types. Initially, all these colonies were screened for tylosin production using Bacillus subtilis bioassay and it was found that only morphologically altered colonies (UV-1, UV-2, UV-3, UV-4, UV-5 and UV-6) indicated tylosin production. Furthermore, HPLC analysis revealed an increase of 2.7 fold in tylosin yield (1500 mg/l) by one of the morphological mutants i.e., UV-2 in complex medium which was improved to 1750 mg/l as compared to the wild type strain (620 mg/l) by optimizing fermentation conditions in chemically defined media. The values of different kinetic parameters such as dry cell mass (21 g/l), maximum specific growth rate, μmax (0.052 h-1) and specific tylosin productivity qp (1.36 mg/g/h) were also higher in mutant UV-2 as compared to wild type strain. To further improve the stability and productivity of mutant UV-2, it was subjected to another round of mutagenesis by gamma irradiation using 60 Co source. As a result of this treatment, another morphologically altered mutant (γ-1) with an enhanced expression of tylosin up to 2500 mg/l in complex medium was obtained. Use of chemically defined media promoted tylosin production up to 3800 mg/l by this mutant γ-1 in the presence of glucose+lactose (45 g/l) and sodium glutamate (12.5 g/l) as carbon and nitrogen sources, respectively. In addition, much higher value of qp (3.34 mg/gh) was observed in case of mutant γ-1 as compared to wild strain (0.81 mg/gh). Moreover, UV irradiation associated changes were observed to be unstable with loss of tylosin expression whereas, mutant γ-1 displayed high stability on subsequent culturing even after 3 years of storage at -72°C. Furthermore, solid state fermentation system (SSF) was also developed for tylosin production with mutant γ-1 and wild type strains by using various economically cheaper and easily available agro-industrial wastes. Wheat bran as solid substrate gave highest production (2500 μg of tylosin/g substrate) by mutant γ-1 against wild type strain (300 μg tylosin/g substrate). The tylosin yield was further improved to 4500 μg/g substrate at optimized conditions of 70% moisture level, 10% inoculum (v/w), pH 9.2, temperature 30°C, supplemented with lactose and sodium glutamate on 9th day. Wild type strain displayed reduced production of tylosin (655 μg tylosin/g substrate) in SSF even after optimization of process parameters. This study has shown that solid state fermentation system significantly enhanced the tylosin yield by mutant γ-1. In this way, an overall increase of 6.87 fold in tylosin yield was achieved through a combination of UV and gamma irradiation mutagenesis and fermentation screening by mutant γ-1. Additionally, to acquire some more knowledge about enhanced expression of tylosin in mutant γ-1, molecular studies were attempted to explore any change in the regulatory genes (tylQ, tylP, tylS, tylR & tylT) of tyl cluster. Expression analysis by RT-PCR revealed that tylQ was switched off earlier in mutant γ-1 as compared to wild type strain, although there was no change in the sequence of tylQ gene from both the strains. On the other hand, the analysis of tylP indicated no change in expression pattern between wild type and mutant γ-1 strains but there was difference of a single base and a substitution mutation of T A was recorded at position 214 in 420bp product of tylP gene. Moreover, the deduced protein sequences of tylP gene from wild type and mutant γ-1 indicated that the point mutation resulted in the change of single amino acid i.e., serine to threonine (S T) at position 72. Furthermore, RT-PCR remained unsuccessful to detect the expression of three other regulatory genes i.e., tylS, tylR and tylT. So, routine PCR was used to amplify these genes, which revealed that there was no change in the nucleotide sequence of these genes either from mutant γ-1 or wild type strain. However, the sequences of these genes could be a valuable addition in databases because these were not available previously for this particular strain of Streptomyces fradiae NRRL-2702.