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Thesis Info

Author

Asad Khan

Supervisor

Abdul Qadus Abbasi

Department

Department Institute of Information Technology, QAU

Program

MSc

Institute

Quaid-i-Azam University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Page

67

Subject

Information Technology

Language

English

Other

Call No: DISS / MSC / IT /237

Added

2021-02-17 19:49:13

Modified

2023-01-06 19:20:37

ARI ID

1676715465932

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3 پنجابی دی کلاسیکی شاعری

پنجابی دی کلاسیکی شاعری

 

                پہلی گل ایہہ وے پئی کلاسیکی شاعری کس شاعر نوں آکھدے نیں؟ کیوں جے کلاسیکی دا عام طور تے مطلب پرانی شاعری دا تصور کیتا جاندا اے ایہہ شاعری ڈھیر مدتاں تک زندہ رہندی اے ایہدے چن نوں گرہن نہیں لگدا اے پت جھڑ دے جثے وچ نہیں آوندی، رتاں دی ضد، عالم تے جاہل دی سوچ، بولیاں دی دوج کلاسیکی شاعری دی جند نوں موت وی نقصان نئیں پہنچا سکدی۔

                پرانی شاعری مدتاں تک کس طرح زندہ رہندی اے؟ ایہدا جواب اے کہ پرانی شاعری وچ جیہڑا روپ ہووے جے اوہ فانی نہ ہوے جیہڑے سچائی ہوئے اوہ ہٹ دھرمی اتے قائم نہ کیتی گئی ہوے ایہدی ڈوہنگائی دل وی ڈوہنگائی وانگر ڈونگھی ہووے ایہدے جذبے خاص حداں توں باہر نہ ہون ایہدے وچ جیہڑا جوش ہوئے اوہ وقتی اوبال نہ ہوے فیر سمجھ لو پئی پرانی شاعری کلاسیکی شاعری ہندی اے فیر ایہہ ہر بندہ بشر دے ذہن اتے سوار ہوجاندی اے ہر قوم وچ مقبول ہوجاندی اے ہر زمانے دی بولی وچ اپنا تھاں بنا لیندی اے۔

                اج دی شاعری نوں اسیں جھٹ پٹ کلاسیکی شاعری نئیں آکھ سکدے کیوں جے ایہدا پتا نہیں...

مشائخِ خانقاہ قادریہ

The founder of Khānqāh-e-Chohar Haripur (Qādriya Silsilah) was Khwāja Muhammad Abdul Rahman Chohārvi (1840-1924), who born in Chohar, a village in Haripur District (Pak). Khwāja Muhammad Mehmood ul Rehman (1907-1986) was the Khalīfa and successor of Khwāja Abdul Rehman Chohārwi. After the demise of his Sheikh, he remained benefiting people by connecting them to Allah and ingraining the love of Allah in their hearts. After his death the new Sheikh of Khānqāh-e- Qādriya Chohar was Khwāja Muhammad Ṭayyab ul Rehman (1935-1995). Khwāja Muhammad Ahmed Rehman is the Fourth Khalīfa after Khwāja Abdul Rahman Chohārvi. Khwāja Muhammad Ahmed Rehman is the son of Khwāja Ṭayyab Rahman. In 1995, he was appointed the successor of his father Khwāja Ṭayyab Rahman.

Microbiology and Phage Therapy of Diabetic Foot Ulcer

Diabetic foot infections (DFI) are a major complication of diabetes mellitus. It contributes to the development of gangrene and non-traumatic lower extremity amputations with the life time risk up to 25 %. Since bacteria responsible for chronic wound infections are commonly within polysaccharide matrices known as biofilms, which to a large extent are refractory to antibiotics even when the bacteria are genetically susceptible to their action. In the first part of the study, we identified the neuropathy, ulcer grade, microbial profile, phenotypic and genotypic resistance prevalence of methicillin and ESBL genes in bacterial isolates of DFI patients registered at PIMS, Pakistan. Our results indicated that 46 (92 %) out of 50 patients, had sensory neuropathy. The most prevelant isolate was Staphylococcus aureus (25 %), followed by Pseudomonas aeruginosa (18.18%), E. coli (16.16%), Streptococcus spp (15.15%), Enterococcus spp (9%), Proteus spp (15.15%) and Klebsiella pneumonia (3%). The prevalence of MecA gene was found to be 88 % and 84% phenotypically and genotypically respectively. K. pneumonia had highest percentage of ESBL producers with 66.6 % prevalence by double disc synergy test and 100 % for CTX+CL/CAZ+CL by combination disc test. Pseudomonas aeruginosa had highest (100 %) number of metalo β-lactamase producers by EDTA synergy disk test. Overall prevalence of bla-CTX-M, bla-CTX-M15, bla-TEM, bla-OXA and bla-SHV genes was found to be 76.92, 76.92, 75.0, 57.69 and 84.6 % respectively in gram negative isolates from DFI. Molecular epidemiology of MecA and ESBL genes were found alarmingly high in DFI, posing one of the major cause of antibiotic treatment failure. In the second part of our study we determined whether combinations of antibiotics and bacteriophage were more effective for the treating biofilm populations of Abstract xvii Pseudomonas aeruginosa [the laboratory strain PA14, and the clinical strain, CFBR2)] on plastic surfaces and layers of human epithelial cells. Two newly isolated bacteriophage NP1 and NP3 at a titer of ~1E8 pfu/ml were added individually or as pairs and/or in combination with 1X MIC, 4XMIC and 8X MIC of ceftazidime, colistin, gentamicin or trobramycin to 48 hours PA biofilms in 6 well polystyrene plates. Parallel experiments were performed with 8-hour biofilm populations of epithelial Naso pharyngeal Detroit 562 (ATCC® CCl-138TM). Treatment with phage reduces the viable density of biofilm populations of P. aeruginosa. biofilms by three orders of magnitude as compared to untreated control. In combination with antibiotics phages are more effective than alone and increase efficacy of the antibiotics for treating bacteria in biofilms. In the third part of study we explored co-evolutionary dynamics of resistance between Pseudomonas aeruginosa 14 and its phages NP1 and NP3. Evolutionary dynamics experiments of single and two phages revealed that PA14 can easily evolve resistance against NP1 phage. NP3 phage maintained for 30 serial transfers and we observed host range in evolved bacteria. In cocktail, both phage support each for their long term maintenance in serial transfer experiments. Also, cocktail delayed the evolution of resistance and sustained high phage infectivity, suggesting phage cocktail is promising strategy to control or slow down evolution of resistance in bacteria against bacteriophages.