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Home > Phylogenetics of Selected Mentha Species on the Basis of Rps8, Rps11 and Rps14 Ehloroplast Genes

Phylogenetics of Selected Mentha Species on the Basis of Rps8, Rps11 and Rps14 Ehloroplast Genes

Thesis Info

Author

Attia Jabeen

Department

Deptt. of Biochemistry, QAU.

Program

Mphil

Institute

Quaid-i-Azam University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2011

Thesis Completion Status

Completed

Page

69

Subject

Biochemistry

Language

English

Other

Call No: DISS/M.PhIl BIO/2663

Added

2021-02-17 19:49:13

Modified

2023-02-19 12:33:56

ARI ID

1676715577251

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1۔اصلاح معاشرہ میں شعبہ امر بالمعروف و نہی عن المنکر کا کردار

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قوانین حدودوقصاص کی تنفیذ کیلئے فضا ساز گار بنانے میں اہم فریضہ امر بالمعروف اور نہی عن المنکر کی ادائیگی کا ہے ۔ یہ فردکا بھی فرض ہے ، معاشرے کا بھی اور ریاست کا بھی فرض ہے ۔ ریاست اگر اس فریضہ کو ادا کرے تویہ فریضہ "حسبہ" کا ادارہ سرانجام دیتاہے۔ ریاست ، معاشرہ اور فرد کے اسی فرض کو قرآن مجید نے مختلف انداز میں بیان فرمایا ہے، جیساکہ ارشاد ربانی ہے
﴿الَّذِينَ إِنْ مَكَّنَّاهُمْ فِي الْأَرْضِ أَقَامُوا الصَّلَاةَ وَآَتَوُا الزَّكَاةَ وَأَمَرُوا بِالْمَعْرُوفِ وَنَهَوْا عَنِ الْمُنْكَرِ وَلِلَّهِ عَاقِبَةُ الْأُمُورِ۔﴾390
"یہ وہ لوگ ہیں کہ اگر ہم زمین میں ان کے پاؤں جما دیں تو یہ پوری پابندی سے نماز قائم کریں ، زکوٰۃ دیں ، اچھے کاموں کا حکم دیں اور برے کاموں سے روکیں اور تمام کاموں کا انجام اللہ کے اختیار میں ہے۔ "
اس آیت مبارکہ کی تفسیر بیان کرتے ہوئے صلاح الدین یوسف لکھتے ہیں
"اس آیت میں اسلامی حکومت کے بنیادی اہداف اور اغراض و مقاصد بیان کیے گئے ہیں جنہیں خلافت راشدہ قرون اول کی دیگر اسلامی حکومتوں میں بروئے کار لایا گیا اور انہوں نے اپنی ترجیحات میں ان کو سر فہرست رکھا تو ان کی بدولت ان حکومتوں میں امن و سکون بھی رہا اور رفاہیت و خوش حالی بھی رہی اور جب تک مسلمان مملکتیں قرآن کے بتلائے ہوئے اصول کے مطابق اقامت صلوۃ و زکوٰۃ اور امر بالمعروف اور نہی عن المنکرکا اہتمام نہیں کریں گی اور اپنی ترجیحات میں ان کو سر فہرست نہیں رکھیں گی ۔ وہ فلاحی مملکت کے قیام میں بھی کامیاب نہیں ہو سکیں گی۔ "391
سورۃ حج کی اس آیت مبارکہ کی مخاطب اسلامی حکومت ہے ۔امر بالمعروف اور نہی عن المنکر ایک اسلامی حکومت...

Analysis of Quarantine Service Quality on Ship Crew Satisfaction during the Covid-19 Pandemic at Belawan Sea Port

This study aims to analyze the quality of quarantine services on ship crew satisfaction during the Covid-19 pandemic at the Belawan Sea port. This study is a quantitative study with a cross sectional method which aims to analyze the quality of quarantine services on satisfaction during the COVID-19 pandemic at the Belawan Sea Port using the ServQual approach which consists of physical evidence, reliability, responsiveness, assurance, and empathy. The results of the study are expected to provide input regarding the quality of quarantine services provided by the Class I Medan KKP to ship crews during the COVID-19 pandemic. Based on the ServQual analysis, the results show that the satisfaction of the crew of the quarantine service at Belawan Port is very satisfying with a suitability level of 107.56% (the average service / reality score is 4.00 and the expectation score is 3.72). It is suggested to improve coordination and more intensive advocacy with shipping agents as intermediaries between officers and skippers as crew so as to reduce the length of service waiting time, in order to increase the number of human resources carrying out document checks on board, involving female medical officers for health checks The crew who came from affected countries/ports to facilitate information retrieval, ordered the boarding officers to bring Personal Protective Equipment when interacting with the crew.

Microbial Degradation of Polyester Polyurethane

In this report, Polyurethane (PU) degrading microorganisms (fungi and bacteria) were isolated from soil through enrichment. The isolated fungal strain was identified by examination of colony morphology i.e. color, size and colony diameter and shape, color, size and structure of conidia, hyphae, conidiophores and conidial head as Aspergillus tubingensis. PU films incubated for one month on MSM-Agar plates inoculated with A. tubingensis demonstrated visible signs of degradation in terms of changes in color and flexibility. Thick mycelial growth and adherence of fungal biomass with surface of PU was confirmed by scanning electron microscopy (SEM). Fourier transformed infra-red spectroscopy (FTIR) spectrum of the treated PU film, when compared to that of untreated control revealed changes in important functionalities. Two bacterial strains isolated from the same soil were identified as Bacillus subtilis MZA-75 and Pseudomonas aeruginosa MZA-85 by colony morphology, microscopy, biochemical characterization and 16S rRNA gene sequence analysis. The degradation of PU film pieces exposed to both strain MZA-75 and MZA-85 was investigated by SEM, FTIR and gel permeation chromatography (GPC). SEM micrographs of PU film pieces, treated with strains MZA- 75 and MZA-85, showed alterations in the morphological features of surface. FTIR spectrum demonstrated rise in organic acid functional groups and fall in ester functionality. GPC results revealed increase in polydispersity, which shows that long chains of polyurethane polymer are cleaved into shorter chains by microbial action. Increase in cell growth and CO 2 concentration detected through Sturm Test, in comparison to control further elaborate the degradative capability of strains MZA-75 and MZA-85. MZA-85 was found capable of producing cell associated esterase measured on the basis of p-Nitrophenyl acetate (pNPA) hydrolysis assay. Time course study for cell associated esterase in the presence and absence of PU in MSM broth revealed that this enzyme is induced by the presence of PU in the medium. Crystal violet staining and SEM results shows that MZA-85 forms biofilm on the surface of PU. In case of MZA-75 increase in both cell bound and extracellular esterases was observed in the presence of PUR films in MSM as compared to control when analyzed through p-Nitrophenyl acetate (pNPA) hydrolysis assay. PUesterase was purified from xiithe MZA-75 by using Sephadex G-75 column chromatography. The purified enzyme gave single band on SDS-PAGE corresponding to molecular weight 51 KDa. Substrate specificity analysis was done using p-Nitrophenyl acyl esters of varying carbon numbers. Maximum esterolytic activity was observed in case of p-Nitrophenyl butyrate (C 4 ). Analysis of the cell free supernatant by GC-MS, revealed that 1, 4-butanediol and adipic acid monomers were produced as result of degradation of PU by both MZA-75 and MZA-85 and both the strains were capable of utilizing these intermediates as carbon source. Both MZA-75 and MZA-85 are subject to further studies to understand their interaction with PU completely, which may be helpful in PU bioremediation and biochemical monomer recycling from PU wastes.