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Genotoxic Environmental Risk Assessment of Pesticides Using Molecular Biomarkers

Thesis Info

Author

Bhalli Javed Ahmad

Department

National Institute for Biotechnology and Genetic Engineering

Program

PhD

Institute

Quaid-i-Azam University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2009

Thesis Completion Status

Completed

Page

iv,147

Subject

Biotechnology

Language

English

Other

Call No: DISS/Ph.D BIO/2362

Added

2021-02-17 19:49:13

Modified

2023-01-06 19:20:37

ARI ID

1676715720634

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عشقی الہاشمی

عشقی الہاشمی
عشقی الہاشمی(۱۹۰۹ء ۔۱۹۸۳ئ)کا اصل نام جعفر علی اور عشقیؔ تخلص کرتے تھے۔ عشقیؔ سیالکوٹ کے سادات نقوی خاندان میں ہوئے۔ آپ عربی فارسی میں خدا داد قابلیت رکھتے تھے اور علومِ شرقیہ کے بہترین اساتذہ میں شمار ہوتے تھے۔ عشقیؔ نے شاعری میں علی طالب الہٰ آباد ی اور لسان الہند مرزا ہادی عزیز لکھنوی سے فیض حاصل کیا۔ سیالکوٹ میں عشقیؔ کے بہت زیادہ شاگرد تھے۔ جنھوں نے اُردو شاعری میں اعلیٰ مقام حاصل کیا۔ اصغر سودائیؔ اور تابؔ اسلم جیسے کاملِ فن شعرا عشقیؔ کے تلمذ میں رہے۔(۴۴۱)
آپ نے مجلہ در’’نجف‘‘ میں بحیثیت مدیر معاون کام کیا۔ ’’شبابِ اردو‘‘ ،اور’’نوروز‘‘ کی ادارت بھی سنبھالی ۔اور امر تسر کے ہفت روزہ ’’مجلہ آرٹ‘‘ کے مدیر بھی رہے۔ (۴۴۲) ’’سر شک بہار‘‘ ،’’مطلع الانوار‘‘ ،’’سوزو ساز‘‘ ،’’سہا و سمن‘‘ اور ’’غزلستان‘‘ عشقیؔ کے چار شعری مجموعے ہیں۔’’العروض ‘‘تصنیف میں فنِ شاعری پر تنقید اور تبصرے شامل ہیں۔(۴۴۳)
عشقیؔ روایتی شاعر ہیں ان کے ہاں کوئی جدت نظر نہیں آتی۔ عشقی ؔ کے اسلوب پر دبستان دہلی اورلکھنو کے اثرات بھی دیکھے جا سکتے ہیں ۔ اُن کی غزلیات چھوٹی اور لمبی بحروں میں ہیں ۔شاعری میں قافیہ اور ردیف پر بہت زور دیتے ہیں ۔ان کی اکثر غزلیات کی طویل ردیفیں ہیں ایسا لگتا ہے جیسے وہ شاعری پر قافیہ اور ردیف کو فوقیت دیتے ہیں ۔ مذکورہ بالا خامیوں کے باوجود عشقیؔ کے ہاں آفاقی موضوعاتِ شاعری بھی موجود ہیں۔ اخلاقیات،رجائیت،قومیت،حقیقت پسندی،اصلاح ،عشقِ مجازی اور عشقِ حقیقی عشقیؔ کی شاعری کے اہم موضوعات ہیں۔ اس حوالے سے نمونہ کلام ملاحظہ ہو:
قوم پر جب زوال آتا ہے

نوجوان بے لگام ہوتے ہیں

جن کو جینے کا آ گیا طریق

ان کے اونچے مقام ہوتے ہیں

جو بشر احترام کرتے ہیں

A case study on the Healthcare Workers in Karachi: The Effect Of Post-Covid Stress On Anxiety and Coping Mechanisms

Abstract The aim of this research was to examine the impact of post-COVID stress on the anxiety and coping mechanisms of healthcare workers. A phenomenological approach was utilized to gain a deeper understanding of the personal experiences of the participants. The results of the study revealed the challenges faced by healthcare workers in the aftermath of the pandemic and the coping strategies they employed to manage stress. These findings provide valuable insights for healthcare organizations and policymakers in their efforts to support the mental well-being of healthcare workers. Keywords: Post-covid-19, anxiety, depression, infection, mental health, pandemics, frontline workers, qualitative study, covid aftermaths

Date Palm Diversity in Pakistan and its Relationship With World Dates Germplasm for Exploring the Center of Origin of Phoenix Dactylifera L.

Date Palm, (Phoenix dactylifera L.) often called ‘nakhl’, ‘khajor’, or ‘khaji’ belonging to the family Arcaceae (Dransfield et al., 2008) is an evergreen tree. It is the hardiest among tropical fruit trees and exceeds most other fruit crops in productivity and adaptability. The characterization of date palm at the genetic level supplemented with phenotypic character is of great concern as an important step towards efficient conservation, maintenance and utilization of the existing genetic diversity. The present research work was carried out to investigate the phenotypic variability and genetic diversity of the date palm germplasm present in the 16 districts of Pakistan. A planned survey of date palm growing regions of Pakistan in 4 provinces (Punjab, Sindh, Khyber Pakhtunkhwa and Balochistan) and Azad Jammu and Kashmir was conducted for collecting leaf and fruit samples of date palm. Young and healthy leaves were used to extract DNA whereas seeds were extracted from fruit for recording qualitative and quantitative morphometric diversity. In total, 2460 seeds of 82 accessions were included in morphometric study. About 139 date palm accessions were evaluated for genetic variability and population structure. The recorded data were analyzed using a set of different statistical tools for making significant interpretation. A total of 19 traits (5 quantitative and 14 qualitative) enabled an assessment of phenotypic diversity and structure. The measured traits: dorsal area, lateral area, embryo to apex length, embryo to base length and their percentage, seed base, micropyle position, seed wings and frequency of wings were more variable. PCA grouped all the accessions according to their phenotypic relations and morphometric characteristics. Cluster analysis distributed all the 82 Pakistani date palm accessions into their three original groups. The results reflect huge seed morphometric variation and presented seeds as a powerful tool for phenotyping. Furthermore, our study also exhibited strong resemblance and possible relationship between some groups of accessions. All 18 SSR primer pairs successfully analyzed molecular genetic variation and produced multiple fragments in the Pakistani collection as well as in date palm germplasm representing 10 different date palm growing regions of the eastern and western world. These set of markers proved themselves as highly polymorphic for genetic diversity estimation and population structure within and among all the geographically spaced population of xvii worldwide accessions. The findings of implemented markers revealed 2-23 alleles per locus with high level of expected heterozygosity in the accessions collected from Pakistan. The most informative SSR marker in this study was mPdCIT078. In addition, cultivar identification key were established. It allowed resolving the difference and constructs the relationships of the same name or vice versa. Our results also suggested the existence of population subdivision at the regional level. Population from semi arid – monsoonal or canal irrigated (seasonal or perennial) belt of Punjab (Bhakkar, Muzafargarh and Rahim Yar Khan) and dry arid ecological zones of Balochistan (Kech, Gwadar) are stable in their structure whereas DAPC distributed 342 accessions of 10 date palm growing countries into two major geographical groups (eastern and western). It is concluded that Pakistani germplasm has huge diversity and genetic pool were close to the eastern world especially Iraq and Oman.