اُردو فِکشن میں جدید اصناف
(مائیکرو فکشن،افسانچہ،فلیش فکشن کا تحقیقی جائزہ)
منیرعباس سپرا،پی ایچ ڈی سکالر
انسان کو شروع سے ہی کہانی سے دلچسپی رہی ہے کہانی کی تاریخ بھی اتنی ہی قدیم ہوگی جتنی انسان کی ہے کہانی کی تاریخ انسانی زندگی اور اس کی بڑھتی ہوئی پیچیدگیوں کی تاریخ ہوتی ہے جس میں انسان کے کارناموں کی روداد ہوتی ہے جس میں اس نے اپنے ماحول معاشرے کی کسی قوت سے مقابل اگر کامیابی حاصل کی ہو ۔یہی کہانی انسان کے احساس برتری کی تسکین کا ذریعہ بنتی ہے اور اس طرح کہانی کا تعلق ہماری فطرت سے بھی ہے۔
کہانی دنیا کا قدیم ترین فن ہے انسانی قوت تخلیق نے سب سے پہلے قصہ کی تخلیق کی اور داستان قصہ گوئی کی شکل میں موجود تھی پھر قصے کہانیوں نے جدید عہد کے علائق تک انسان کے ساتھ ہی کہانی نے بھی بہت سے مراحل طے کیے داستان گوئی سے ناول ،ناولٹ اور افسانے سے مختصر کہانیوں (مائیکرو فکشن ،افسانحہ ،فلیش فکشن) کی طرف اردو فکشن کا یہ ارتقائی سفر جارہی ہے کیونکہ فکشن میں یہ تغیر تبدل ادب کے ارتقاء کیلئے ناگزیر ہے ارتقاء کا یہی سفر سماج میں بھی جاری رہتا ہےپہلے کی نسبت لوگوں کے پاس فرصت کے لمحات کم ہیں مشینی، صنعتی اور ٹیکنالوجی کی ترقی نے لوگوں کو مصروف کردیا ہےاسی مصروفیت کی حالت میں لوگ ادب سے بھی لطف اندوز ہونا چاہتے ہیں اسی لیے عام قارئین داستان ناول وغیرہ کی ضخیم کتب کی بجائے کم وقت میں کہانیوں سے وہی لذت کشید کرنے کیلئے مختصر کہانیوں( مائیکرو فکشن ،ا فسانحہ ،فلیش فکشن وغیرہ) کی طرف راغب ہو رہے ہیں آج کے ٹیکنالوجی ،مشینی اور گلوبلا ئزیشن کے دور میں معمولیات زندگی میں...
In the early days of Islam, either of the married couple entering into Islam does not affect their marriage contract and it continued to be valid even after the Emigration of the Holy Prophet (peace and mercy be upon him) to Madina. In fact, it has been observed until Hudhabiya Truce was signed. It was also included the terms if someone from Quraish without accompanying the wali approaches the Holy Prophet (Peaceand Mercy be upon him), he/she will be returned to Makkah. After this agreement, many women came to Madina and embraced Islam. But their spouses and relatives followed them their way to Madina. They claimed them back to Makkah. In this respect, Allah almighty revealed a verse of Surah Mumtahina, which is an express evidence that such believing women must not be returned to their former infidel husbands. They were commanded so to marry believing husbands after their separation from their disbelieving spouses. Through this verse, Muslim husbands were forbidden to stay and have conjugal relations with their nonbelieving wives, too. Similarly, every believing wife was forbidden to reside with her disbelieving husband. The companions abided by the ruling in its entirety and separated from their non- believing spouses. Jurists have derived many instructions from the verse 10 of Surah Mumtahina, which are discussed in this paper.
Pakistan is reporting high number of polio cases during the last few years and the disease is continuously spreading. Poliovirus 2A protease (2Apro) is multifunctional proteinase performing vital roles during viral replication and cellular damage. RNA from polio infected blood samples and also from live attenuated polio vaccine was extracted followed by 2Apro amplification cloning and sequencing. The sequences were compared against the standard consensus 2Apro sequence obtained from Genbank to analyse mutations. In order to understand more concerning PV2Apro the protein was over-expressed in bacteria following amplification using sense and antisense primers and cloning in pET15b. Several expression hosts were tested and BL21 (DE3) pLysS cells gave the best expression of PV2Apro with minimal unwanted protein expression following IPTG induction. The 2Apro protein was purified to homogeneity using column chromatography, its solubility determined and its molecular weight and composition determined by MALDI-TOF mass spectrometry. The purified protein had a slightly lower molecular weight than predicted. Several attempts were made to concentrate the protein using different buffers for ion exchange and crystallization procedures. However after every buffer change to reduce imidazole concentration, protein was gradually degraded. The 3D structure of 2Apro was determined and optimized by homology modelling followed by an energy minimization protocol. Structural validation programs VADAR and QMEAN were used to verify the 2Apro model. Analysis of protein stability changes of poliovirus 2A protease-mutated sequences using various servers was also performed. Furthermore, mutation pattern, intrinsic disorder regions (IDRs), hydrophobic regions, drug binding sites (DBS) and subcellular localization were identified. Hydrophobicity results confirmed the suitability and reliability of 2A protease as a potential drug target. Less IDRs were observed in the protein. In order to predict the molecular basis of interaction of small molecular agonists, docking simulation was performed on a structurally diverse dataset of known inhibitors of PV2Apro. Docking results were employed for missense mutations to predict extremely damaging to the protein structure as well as the function of the protease. Intrinsic disorder regions (IDRs), drug binding sites (DBS), and protein stability changes upon mutations were also identified among them. Our results demonstrated dominant roles for Gly 1, Lys 15, His 20, Cys 55, Cys 57, Cys 64, Cys 109, Gly 110 and Gly 111 within the binding pocket of PV2Apro. Upon subjecting these sites to single nucleotide polymorphism (SNP) analysis, we observed that out of 155 high risk SNPs, 139 residues decrease the protein stability. We conclude that these missense mutations can affect the functionality of the 2A protease, and that identified protein binding sites can be directed for the attachment and inhibition of the target proteins. Mutations detected in this study were also found to be interacting with the inhibitors proving them to be valuable tool for in formulating new drugs to inhibit polio virus replication. We have identified residues which would be helpful in the development of future selective drugs along with as important inhibitors to inhibit the replication of poliovirus.