چودھری محمد علی رودولوی
افسوس ہے کہ پچھلے دنوں اردوزبان کی دوبڑی شخصیتیں بھی ہم سے جدا ہوگئی۔ ایک چودھری محمد علی رودولوی اور دوسرے عبدالمجید سالک۔
چودھری صاحب اردو کے نامور ادیب،پختہ قلم اورکہنہ مشق انشاء پرداز تھے۔ایک زمانہ میں ان کی تحریریں بڑی دلچسپی اورشوق سے پڑھی جاتی تھیں۔ زبان دانی کے ساتھ ان کی تحریر کاسب سے بڑاکمال یہ تھاکہ رکیک سے رکیک بات یا خیال کو اس اندازسے لکھتے تھے کہ ذوقِ سلیم کوگھناونی معلوم نہیں ہوتی تھی۔’’اتالیق بی بی‘‘، ’’کشکول‘‘،’’فقیر محمدعلی شاہ‘‘ان کی مشہور تصنیفات ہیں۔ ان کے علاوہ متعدد افسانے اوردوچارکتابیں بھی ان کی یادگار ہیں۔ طبعاً بڑے ہنسوڑ، زندہ دل اور شگفتہ انسان تھے۔تعلقہ دارہونے کی وجہ سے زندگی بڑے عیش وآرام میں گزری۔ آخر عمر میں خوف خداکاان پر بڑاغلبہ ہوگیا تھا، نماز توخیر پابندی سے پڑھنے ہی لگے تھے اورحج بھی کرآئے تھے مگر آخرت کے ڈر سے بے چین رہتے تھے۔ اگرچہ خاندانی طورپر امامیہ فرقہ سے تعلق رکھتے تھے لیکن تعصب ان میں نام کوبھی نہ تھا۔ اہل سنت والجماعت مسلمانوں کے ساتھ نمازانھی کے طریقے پر پڑھتے تھے۔ اس سلسلہ میں’’میرامذہب‘‘کے نام سے انھوں نے ایک کتاب بھی لکھی تھی۔خاتمہ بھی ایسا ہی اچھا ہوا۔۱۰/ستمبر کوبروزپنج شنبہ درودپڑھتے اوراﷲ کے نام کا وردکرتے کرتے جان جان آفریں کے سپرد کردی۔
الھم اغفرلہ وارحمہ۔
[اکتوبر۱۹۵۹ء]
Literally Bai't means solemn assurance to do something and absolute submission to one's commands. All the believers have declared to follow the commands of Allah Al-Mighty and act upon the guideline of His Holy Prophet (P.B.U.H). In the early days of Islam, every person intending to embrace Islam use to take an oath of obedience and loyalty to the orders given by the Prophet (P.B.U.H). The person gave his hand in the hands of the Prophet (P.B.U.H). Afterwards, this sort of oath was introduced in many kinds; one of them is "Bai't Islah or Bai't Al Tuba". The Bai't is in accordance with the spirit of Islamic teachings. The scholars of Islam in majority have accepted its authenticity as discussed in the article under reference.
Current Ph.D. dissertation comprises of five chapters. A brief overview of every chapter is presented to provide an outline of the research contribution which is done in this thesis. Chapter 1 provide details regarding the general introduction of Computer-Aided Drug Discovery (CADD). In particular, it focuses on molecular modeling, Structure-based and Ligand-based drug design methods. Chapter 2 is related to the inhibitory studies of CK2 protein. In this study docking, 3DQSAR and MD simulation are reported with an emphasis on how each method is utilized to gain insight at the molecular level. Alignment obtained from the top-ranked conformation of inhibitors was used for developing the statically significant 3D-QSAR model. A further model was validated through the acceptable extrapolative ability to support both training and test set compounds. Structural changes were observed with the help of MD simulation produced by different substitution on inhibitors. Based on QSAR and MD results some new compounds were also designed. Chapter 3 deals with an effort to identify new pharmacological probes with high specificity for EPAC2 inhibition, using various modern computational tools. Initially, a comprehensive assessment of different scoring function and placement methods was conducted, and effective pharmacophore-based virtual screening protocol was set for the screening of EPAC2 inhibitors. The optimal model with the best six features brought forth and used as a 3D query for virtual screening to retrieve potential inhibitors from Maybridge, Cambridge, and NCI database. The screened compounds were subsequently subjected to molecular docking and 2D-QSAR studies. Finally, 22 top scored compounds with different scaffold having interactions with active site residues were predicted as a lead candidate who may become the starting point in the development of novel and potent EPAC2 inhibitor. Chapter 4 comprise of detailed work on Aurora Kinase B inhibitors. The inhibition of Aurora kinase B is necessary for the treatment of cancerous diseases. By utilizing integrated computational techniques, including 3D-QSAR modeling, pharmacophorebased virtual screening, and MD simulation, we proposed some novel compounds as potential Aurora kinase B inhibitors. Additionally, the highly significant 3D-QSAR model was developed using CoMFA and CoMSIA method. Moreover, the obtained best pharmacophore model was used for virtual screening against a database of over 30 million drugs like molecules which were randomly selected from large commercially available databases, i.e. Chembridge, National Cancer Institute database (NCI), Maybridge and ZINC database. The hit compounds were further filtered with molecular docking, and their biological activities were predicted using the CoMFA model. Visual inspection, docking calculations, and MD simulation revealed that novel leads established better binding affinities with Aurora kinase B. Chapter 5 deals with the antidiabetic potential of naturally occurring flavonoids. In this study, 9 selected flavonoids compounds were evaluated for their binding affinities with PKA via molecular docking study. According to in silico prediction, these compounds are involved in PKA dependent pathway which was further explored by in vitro mice islets.