اعلیٰ تعلیم حاصل کرنے کے بعد فکر معاش اور غم روزگار فطری بات ہے۔ لیکن پروفیسر عبدالحق نے جس طرح تن دہی اور جس مساعدت کے ساتھ اپنا علمی سفر طے کیا اس طرح سےا نہیں ملازمت میں بھی ہولیات فراہم ہوگئیں۔ ایک توان کی علمی لیاقت اور ادبی صلاحیتیں دوسری شریف اساتذہ کی سر پرستی اور پھر پروفیسر خواجہ احمد فاروتی جیسے جو ہر شناس کی تیز نگاہ جو ہر قابل کو پہچان گئی۔ ابھی پروفیسر عبد الحق پی۔ ایچ۔ ڈی کے وایوا سے فارغ ہی ہوئے تھے کہ پروفیسر محمد حسن نے پروفیسر خواجہ احمد فاروقی کے ایما پر پروفیسر محمود الہی صدر شعبہ اردو گورکھپور یو نیورسٹی کو خط لکھا جس میں پروفیسر عبدالحق کو دہلی طلب کرنے کے لیے لکھا تھا۔ یہاں شعبہ اردو دہلی یونیورسٹی دہلی میں تین مہینے کے لیے ایک عارضی جگہ خالی تھی۔ ڈاکٹر شرافت حسین مرزا جو ریسرچ اسٹنٹ کے طور پر کام کر رہے تھے وہ تین ماہ کی رخصت پر اپنے وطن جانے والے تھے۔ خواجہ احمد فاروقی کی یہ خوبی تھی کہ وہ کسی اسامی کو خالی رہتا نہیں دیکھ سکتے تھے۔ اس سال 1965 ء میں صدیق الرحمان قدوائی لیکچرار شعبہ اردو اپنے ریسرچ کے کام سے تین ماہ کی رخصت پر چلے گئے ۔ ان کی جگہ گورکھپور سے ڈاکٹر فضل الحق کو لیکچرر مقرر کر دیا گیا۔ پروفیسر عبدالحق کو بھی تین ماہ کے لیے دہلی طلب کیا گیا تھا لیکن جب وہ 17 دسمبر 1965ء کو دہلی تشریف لائے تو معلوم ہوا کہ ڈاکٹر شرافت حسین مرزا نے چھٹی کی درخواست واپس لے لی ہے تو پروفیسر عبد الحق واپس جون پور جانے کے لیے تیار ہو گئے لیکن خواجہ احمد فاروقی نے از راہ شفقت انہیں روک لیا اور تقریبا ًچھ ماہ کے بعد فاروقی صاحب(Post-Doctoral Fellowship) کی کوششوں سے...
There is major influence of quotes of Sahabaa’h and taa’baeen on Islamic Jurisprudence. A plain study of Fiqh presents the role of tab’aeen and their quotes in method of extraction. There quotes not mere only help us in various matters of virtues, but also in family issues and others. As a matter of fact, a major number of fatawas are based on their quotes or practice as well. Moreover, every school of thought in fiqh narrates their sayings in favour of their vision, I.e. Hanfiya, Shafiya and Malkiya used to narrates their quotes and sayings. Family life is base of any nation or civilization. Books of fiqh also discussed numerous issues of family life and their solutions in the light of Shariaah. In this article a view of tab’aeen’s quotes related to family issues will be discussed. It will support in understanding the importance of their quotes in Islamic Jurisprudence and its compilation. Relevant examples of different school of thought will be discussed from their own books respectively
Aspergillus carbonarius (NRRL–369) and Aspergillus oryzae from Aspergillus genus as well as Cladosporium carrionii and Cladosporium resinae (NRRL–6437) from Cladosporium genus were selected for the present study. Nutrient media were optimized for the growth and production of secondary metabolites. Out of five different media used, A. carbonarius and A. oryzae produced relatively more metabolites in Czapek–dox (Glucose and Starch) broth media (CGSB). Whereas; C. carrionii and C. resinae produced relatively more metabolites in Czapek yeast extracts broth (CYB). To further increase secondary metabolites productivity, two additional chemical compounds (suberoyl anilide hydroxamic acid; SAHA and 5–azacytidine; 5–AZA) were also used as chemical inducers for all fungi except C. carrionii. A dose of 10 μM/100 mL of SAHA resulted in higher secondary metabolites production from Aspergillus species and 15 μM/100 mL of SAHA resulted in higher secondary metabolites production from C. resinae. While a dose 15 μM/100 mL of 5–AZA resulted in higher secondary metabolites production from all the species. Secondary metabolites produced were then studied for its respective biological activities. In antibacterial assay a dose of 500 μg/mL of ethyl acetate extracted from A. carbonarius inhibited the growth of B. subtilis (64.5%), while for antifungal testing a dose of 1000 μg/mL ethyl acetate extract inhibited the linear growth of C. glabrata (58.5%). Whereas, in cytotoxic activities, dose of 1000 μg/mL of ethyl acetate extract showed 94% mortality against brine shrimps, while for phytotoxic activities, a dose 1000 μg/mL showed 90% mortality against Lemna. A dose of 500 μg/mL of ethyl acetate extracted from A. oryzae inhibited the growth of B. subtilis (94%), while for antifungal testing, a dose of 1000 μg/mL of ABSTRACT xxi ethyl acetate extract inhibited the linear growth of M. Canis (84%). Whereas, in cytotoxic activities a dose of 1000 μg/mL of ethyl acetate extract showed 52% mortality against brine shrimps, while for phytotoxic activities, a dose of 1000 μg/mL of ethyl acetate extract showed 67% mortality against Lemna. Furthermore, during the antibacterial assay a dose of 500 μg/mL of ethyl acetate extracted from C. carrionii inhibited the growth of B. subtilis (66%), while for antifungal testing a dose of 1000 μg/mL ethyl acetate extract inhibited the growth of C. albicans (60%). Whereas, in cytotoxic activities a dose of 1000 μg/mL of ethyl acetate extract showed 87% mortality against brine shrimps, while for phytotoxic activities, a dose of 1000 μg/mL ethyl acetate extract showed 80% mortality against Lemna. Finally during the antibacterial assay a dose of 500 μg/mL of ethyl acetate extracted from C. resinae inhibited the growth of S. aureus (81%), while for antifungal testing a dose of 1000 μg/mL of ethyl acetate extract inhibited the growth of A. flavus (15%), while in cytotoxic activities a dose of 1000 μg/mL of ethyl acetate showed 93% mortality against brine shrimps, while for phytotoxic activities, a dose of 1000 μg/mL of ethyl acetate showed 80% mortality against Lemna. The biological activities indicates that, the extracts from A. oryzae and C. carrionii inhibited the growth of experimental organisms with greater extent as compared to A. carbonarius and C. resinae; therefore, A. oryzae and C. carrionii were further selected for the isolation of pure metabolites. A total of three new and four known metabolites were isolated. Two new metabolites were isolated from A. oryzae while one new and four known metabolites were isolated from C. carrionii using preparative High Performance Liquid Chromatography (HPLC) and column chromatography techniques. The structures of all the compounds isolated were ABSTRACT xxii elucidated using (1D and 2D) NMR, IR and HR–MS techniques. The new metabolites were 6–butyl–3–methylene–2–oxotetrahydro–2H–pyran–4–carboxylic acid (A–41), 6–butyl–3–methylene–2–oxo–3,6–dihydro–2H–pyran–4–carboxylic acid (A–42) and (3S,6S)–3–allyl–6–benzylpiperazine–2,5–dione (D–44) whereas, the known metabolites were 5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one (C–43), 6–(3– methylbut–2–enyl)–1H–indole–3–carboxylic acid (45), 2-(4,6-dihydroxy-3-oxo-1,3- dihydroisobenzofuran-1-yl) acetic acid (46) and 2-(4-hydroxy-1,3- dihydroisobenzofuran-1-yl) acetic acid (47). The two new metabolites (A–41 and B–42) from A. oryzae were selected for the determination of their biosynthetic pathways using [1– 13C] labelled acetate. The [1– 13C] labelled acetate was added to the media on 4th, 5th and 6th days respectively. After the feeding of isotopic [1– 13C] labelled acetate as precursor, the labelled metabolites were isolated using HPLC and the pattern of their incorporation were determined using high field NMR. The basic idea of the present work was to isolate biologically active secondary metabolite(s) from fungi and to produce good quality of antibiotics for the welfare of the society.