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Home > Characterization of Non-Coding Sequence Elements in Tetraodon Nigroviridis and Gasterosteus Aculeatus Through Whole Genome Sequence Somparisons

Characterization of Non-Coding Sequence Elements in Tetraodon Nigroviridis and Gasterosteus Aculeatus Through Whole Genome Sequence Somparisons

Thesis Info

Author

Faiza Riaz

Department

National Center for Bioinformatics, QAU

Program

Mphil

Institute

Quaid-i-Azam University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2011

Thesis Completion Status

Completed

Page

iii, 57

Subject

Bioinformatics

Language

English

Other

Call No: DISS/M. Phil. BIO/2807

Added

2021-02-17 19:49:13

Modified

2023-02-19 12:33:56

ARI ID

1676715876722

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محمد علی جناح [قائد اعظم]

محمد علی جناح
گزشتہ مہینہ جب کہ برہان کی کاپیاں پریس میں جاچکی تھیں مسٹر محمد علی جناح کے انتقال کی افسوسناک خبرملی۔ موصوف پاکستان کے معمار اولین اوراب اس کے قیام کے بعد اس کی ریڑھ کی ہڈی تھے اس بنا پر اہل پاکستان جتنا بھی غم اور ماتم کریں کم ہے لیکن اس سانحہ کاافسوس سب کوہی ہوا کیونکہ اگرچہ کچھ دن اوپر ایک برس ہواوہ ہم سے بچھڑ گئے تھے، تاہم تھے وہ معدن ہند کے ہی ایک گوہر آبدار اور ہندوستان کی شصت سالہ جدوجہد آزادی کی تاریخ کے صفحات ان کے ذکر سے بھی خالی نہیں ہیں۔
مرحوم کوعام طورپر ہندوؤں کادشمن سمجھا جاتاہے حالانکہ مسلمانوں کے قائد اعظم کا اُن پر ایک احسان ہی کیا کم ہے کہ جسے قدامت پرست ہندو خود چاہتے تھے مگرزبان سے اس کا اظہار تک نہ کرسکتے تھے وہ قائداعظم نے خودبخود کردیا یعنی ہندوستان کے آزاد ہونے کا وقت قریب آیاتوایک چھوٹا ساٹکڑا لے کریہ کہہ کر پوراملک ان کے حوالہ کردیا :
سپردم بتومایۂ خویش را
/تودانی حسابِ کم وبیش را
wاوریہاں کے مسلمانوں کوغیرموثرٔاقلیت میں تبدیل کرکے ایسا بے دست و پا بنادیا کہ اب ہندومہا سبھا تک کو ان تہی دستان قسمت پرغصہ آنے اوران کی طرف سے خوفزدہ ہونے کے بجائے ان پرترس آنے لگاہے :
کھیل ہیں دورِآسمانی کے
چنانچہ مرحوم کہابھی کرتے تھے کہ ’’ہندومجھ کواپنا دشمن سمجھتے ہیں لیکن اگر پاکستان بن گیا تووہ ہمیشہ میرے احسان مند رہیں گے اورمیرے مرنے کے بعد ان کومحسوس ہوگا میں ان کادشمن نہیں سچا دوست تھا۔‘‘
اس میں شبہ نہیں کہ مرحوم اپنی ذہانت وفطانت، قانونی اورپارلیمنٹری قابلیت و لیاقت،سیاسی سمجھ بوجھ،خوداعتمادی،قوت تحریر وخطابت،غیر معمولی قوت ارادی،مستقل مزاجی،حاضرجوابی، ان اوصاف وکمالات کے باعث عہد حاضر کے ایک بڑے آدمی تھے اورسیاسی لیڈر کی حیثیت سے ان کادامن...

نقد سند و متن میں شیخ البانی کے تفردات

Shaykh Muhammad Nasiruddin Albani is known as the famous scholar of the twentieth century AD. He served in Hadith for almost 60 years. He has also some particularities in the hadith’s research in which he apposed a lot of scholars. The most important of them is that he has said that some Ahadith of Sahih Bukhari and Sahi Muslim are weak. Similarly, in contrast to the previous muhaddiseen, some weak traditions have said correct and some reliable narrators as weak. Apart from this, there are two particularities of him that are very important in the research world. One is that he has explored many of unknown Ahadith and secondly he has divided the books of Hadith into two parts; weak and accurate. Some detail of these particularities is presented in this article.

Effect of Heavy Metals Contamination of Soil by Industrial/ Domestic Effluents on Size and Activity of Soil Microbial Biomass

Experiments were conducted in order to evaluate the effect of effluent irrigation on heavy metals accumulation in soil and soil microbial properties. The study comprised of three parts, In the first experiment twenty five soil samples each at depths of 0-15 cm and 15-30 cm were collected from Islamabad, Adiala, Taxilla and Wah factory areas and analyzed for physiochemical properties, AB-DTPA extractable and total heavy metals and microbial parameters like soil microbial biomass C, microbial biomass N, soil respiration and activities of enzymes such as dehydrogenase and alkaline phosphatase. Effluent samples from these areas were also collected and analyzed for heavy metals contents and other water quality parameters. The results of effluent analysis revealed that EC, total dissolved solids (TDS) and heavy metals such as Cr, Ni, Cd, Cu, and Fe were above permissible limits while Zn and Pb were within the permissible range. In soil samples, AB-DTPA extractable Fe, Cu and total Cu contents were markedly higher than the critical limits at all the sites. AB-DTPA extractable and total Pb contents were within permissible limits. AB-DTPA extractable Zn was high at 24 sites whereas total Zn was higher than the critical limits at two sites only. Soil pH, organic C, CEC, clay and CaCO 3 contents showed negative but weak correlations with the AB-DTPA extractable metals in soil at both soil depths, while the soil EC had positive. There were negative correlations of total and AB-DTPA extractable heavy metals in soils with soil microbial biomass C, microbial biomass N and the activities of dehydrogenase and alkaline phosphatase, but a positive relationship with soil respiration. Total toxic heavy metals had shown strong correlations with microbial activity parameters. Inthe second experiment, a laboratory incubation experiment was carried out in order to evaluate decomposition pattern of different organic amendments like pea straw and maize straw in soils with variable metal contents, designated as low-, medium- and high- metal soils. Each soil (600 g oven dry equivalent) was incubated at 25 o C and 50 % water holding capacity for 56 days with the following treatments: 1) Control, 2) pea straw 1 %, 3) maize straw 1 %, with four replications. Soil samples were collected at 0, 7, 14, 28, 42 and 56 days after incubation for the measurement of microbial biomass C, microbial biomass N, dehydrogenase, alkaline phosphatase and N mineralization. Soil respiration was measured 1, 2, 3, 5, 7, 10 and 14 days after incubation and thereafter weekly. Results showed a significant decrease in microbial biomass C, biomass N and N mineralization with increasing metal load in soil. Soil respiration rate was in the order: high metal soil > medium metal soil > low metals soil. Significant increases in microbial biomass C, biomass N, NO 3 -N, NH 4 -N and enzymes activities (Dehydrogenase and alkaline phosphatase) were noted with the addition of organic amendment (pea and maize straw) as compared to control in all three soils but the magnitude of increase was maximum in low metal treatment. In the third part of study, a greenhouse experiment was carried out to elucidate the effects of organic amendments on soil microbial parameters, heavy metals bioavailability and the growth of maize plants in two soils with variable metal contents. The treatments applied were 1) control, 2) pea straw 1 %, and 3) maize straw 1 % with 4 replications. Soil samples were collected at the time of pot filling and at sowing, 14, 28 & 56 days after sowing (DAS) of maize and analyzed for soil microbial biomass C, microbial biomass N, dehydrogenase, alkaline phosphatase, NO 3 -N and NH 4 -N. Plant samples were harvested at 56 DAS and data regardingplant height, fresh & dry weight were recorded. Soil and plant samples at harvest were also analyzed for N, P, K, Ca, Mg, Na, and heavy metals contents. Microbial parameters were markedly lower in high metal than in low metal soils. Organic amendments significantly increased all microbial parameters, soil NO 3 -N and NH 4 -N in both the soils. Plant growth in low metal soil was significantly higher than the high metal soil. A significant increase in microbial parameters was noticed with pea straw addition as compared to maize straw and control treatments. Addition of pea straw and maize straw significantly reduced AB -DTPA extractable (Cu, Zn, Fe, Mn, Pb, Cd, Cr & Ni) metals in the soils and their uptake by maize plants. Significant reduction in plant metal uptake was noticed with organic amendments, specifically by maize straw treatment. Overall, in study 2 and study 3, the interaction effects of organic amendments with soils depicted a significantly higher microbial activity parameters in soils amended with pea straw than the maize straw. On the basis of this study the main conclusions drawn are, 1) most of the soils in effluent irrigated field of Rawalpindi/ Islamabad areas possess heavy metals above critical limits 2) microbial biomass and activity parameters in these soils showed a decline with increasing concentrations of total heavy metal in soil and 3) addition of organic amendments improved microbial parameters in metal polluted soils but the magnitude of improvements decreased with increasing heavy metal load of the soil. Thus, the application of organic amendments such as pea and maize straw in effluent irrigated areas is recommended for reducing metal toxicity to microbes, and plants. A close monitoring of the soil and crops in agricultural field irrigated withuntreated wastewater is required on regular basis in order to prevent the degradation of soil and entry of heavy metals into food chain.