مولانا عبدالرزاق ملیح آبادی
افسوس ہے کہ گذشتہ مہینہ ہماری جماعت کے ممتاز رکن اور ندوہ کے نامور فرزند مولانا عبدالرزاق صاحب ملیح آبادی نے وفات پائی، انھوں نے متوسطات تک ندوہ میں تعلیم پائی، اور تکمیل جامعہ ازہر مصر میں کی تھی، علامہ رشید رضا کے خاص شاگردوں میں تھے، ان کا ذوق ابتدا سے سیاسی بلکہ انقلابی تھا، چنانچہ مصر کے قیام کے زمانہ میں قسطنطنیہ جاکر انور پاشا سے ملے، ان کی ملاقات نے سیاست اور آزادی کا نشہ اور تیز کردیا، پہلی جنگ عظیم کے بعد ہندوستان واپس آئے، اور کچھ دنوں تک مولانا عبدالباری فرنگی محلی رحمتہ اﷲ علیہ کے ساتھ رہے، جن کی ذات اس زمانہ میں مسلمانوں کی سیاست کا مرکز تھی، مگر مولانا عبدالرزاق کے خیالات اس زمانہ کی سیاست سے بہت آگے تھے، اس لئے زیادہ دنوں تک یہ ساتھ نہ رہ سکا۔
حسن اتفاق سے اسی زمانہ میں مولانا ابوالکلام کو ایک علمی و سیاسی رفیق کار کی تلاش تھی، اس کے لئے ان کی نگاہ انتخاب مولانا عبدالرزاق پر پڑی اور ان کو انھوں نے کلکتہ بلالیا، اس وقت سے وہ مولانا کے دامن سے ایسے وابستہ ہوئے کہ مرتے دم تک ان کا ساتھ نہ چھوڑا، وہ برسوں مولانا ابوالکلام کے سیاسی اور علمی کاموں میں ان کے دست راست رہے، چنانچہ دوسرے دور کے البلاغ اور ۱ مشہور عربی اخبار الجامعہ کے اڈیٹر مولانا ابوالکلام برائے نام تھے، ان کا پورا کام مولانا عبدالرزاق انجام دیتے رہے، الجامعہ ہندوستان میں عربی کا پہلا معیاری اخبار تھا، جس کی شہرت عرب ملکوں تک تھی، ہندوستان کے مسلمانوں میں عربی ادب و انشاء کا صحیح ذوق پیدا کرنے اور عرب ملکوں سے ان کا رابطہ استوار کرنے میں اس اخبار کا بڑا حصہ ہے، ان علمی و صحافتی مشاغل کے ساتھ سیاسی تحریکوں میں بھی علمی...
Knowledge without its moral disposition is of no benefit. The virtues of knowledge without hidāyat are useless and vice versa. Man is advised to control his behavior as he will be questioned for his deeds. Faith is not a matter of words but of accepting Allah’s will and striving in his cause. Every soul shall have a taste of death and on the Day of Judgment will be paid full recompense for his deeds. The one who is admitted to heaven would attain the object of life. This object is achieved by moral character. It shapes an individual in a way conducive to the unfettered growth of good, virtue and truth in every sphere of life. It gives full play to the forces of going in all directions. Also it removes all impediments in the path of virtue. It eradicates evils from social plan by prohibiting the causes of its appearance and growth, by closing the inlets through which it creeps into a society. It saves from all sorts of human weaknesses and counsels of pseudo-wisdom, self respect that keeps breaking-in and resists all evils. This is attained by exercising highest patience and self restraint. It signifies the entire scheme of life and not any isolated part or parts thereof. Akhlāq develops an attitude in a person by which every moral valuation, every decision as to the practical course for whatever the individual would prefer in his life to take for ultimate success.
In the present research study twenty families segregating autosomal recessive form of hypotrichosis and ectodermal dysplasias, and one X-linked hypohidrotic ectodermal dysplasia have been characterized at clinical and molecular levels. Ten families presented clinical features of various types of isolated hair loss disorders, six isolated nail dysplasias and five ectodermal dysplasias. Genotyping using microsatellite markers established linkage in seventeen families to previously known genes. Subsequently, Sanger cycle sequencing revealed three novel missense/nonsense variants in FZD6, PVRL4 and ELOVL4 genes, and eight previously reported mutations in HR, DSG4, LIPH, LPAR6, RSPO4, EDA, and PVRL4 genes. In two families, SNP-based human genome scan mapped novel homozygous regions on two different chromosomes. Further, exome sequencing identified the first disease causing mutation in a keratin gene. In a family, collected from a remote region of Pakistan, all four affected members manifested coarse, lusterless, dry, and tightly curled woolly hair with sparse eyebrows and eyelashes. Whole Genome Scan (WGS) identified 15 cM genetic interval on chromosome 17q21.2-17q22. Whole exome sequencing identified the first disease causing mutation (p.Leu317Pro) in KRT25 gene. Linkage in eight other families, with hair loss disorders, was established to the genes HR on chromosome 8p21.3, LIPH on 3q26.33-q27.3, DSG4 on 18q21.1 and LPAR6 on 13q14.11-q23.21. DNA sequence analysis identified previously reported mutations including two missense (p.Pro1157Arg, p.Cys690*) in HR, a two base-pair deletion (c.659_660delTA) in LIPH, a large deletion (Ex5_8del) in DSG4 and a missense (p.Asp63Val) in LPAR6. In silico analysis of mutated and normal modelled LPAR6 proteins revealed abnormal phospholipid signaling pathway leading to hypotrichosis. One of the families failed to show linkage to the known genes. The second group of six consanguineous families, segregating five different types of nail abnormalities, was characterized at clinical and molecular levels as well. Two of these families failed to show linkage to the previously reported genes. Human genome scan was performed in one family, which led to the identification of a novel locus on chromosome 4p15.0-4p15.2. DNA sequence analysis in three families identified a Abstract Genetic Mapping and Mutation Analysis of Genes Causing Autosomal Recessive Hypotrichosis and Ectodermal Dysplasias XXVIII novel homozygous missense mutation (p.Gly422Asp) in FZD6 and a recurrent 26 bp deletion mutation (-9- +17del26) in RSPO4 gene. Screening HPGD gene in two families, mapped to Isolated Congenital Nail Clubbing (ICNC) locus on chromosome 4q34.1, failed to detect any potential disease causing sequence variant. In five families, three different forms of ectodermal dysplasias were identified. In two of these families, segregating ectodermal dysplasia syndactyly syndrome (EDSS), screening PVRL4 gene revealed two mutations including a novel nonsense (p.Asp61*) and a previously reported missense (p.Pro212Arg). Another family showed segregation of a rare form of neuro-ichthyotic syndrome in autosomal recessive manner. DNA sequence analysis identified a novel homozygous nonsense mutation (p.Tyr26*) in ELOVL4 gene. In a family with hypohidrotic ectodermal dysplasia (HED), sequence analysis detected a recurrent missense mutation (p.Arg155Cys) in the X-linked EDA gene. The second family segregating autosomal recessive form of HED, screening EDAR gene failed to identify potential disease causing sequence variants. The research work presented in the thesis contributed in publication of the following articles. 1. Raza SI, Dar R, Shah AA, Ahmad W (2014). A homozygous nonsense mutation in the PVRL4 gene and expansion of clinical spectrum of EDSS1. Annals of Human Genetics (In Press). 2. Raza SI, Muhammad D, Jan A, Ali RH, Hassan M, Ahmad W, Rashid S (2014). In silico analysis of missense mutations in LPAR6 reveals abnormal phospholipid signaling pathway leading to hypotrichosis. PLOS One 9: e104756. 3. Mir H, Raza SI, Touseef M, Memon MM, Khan MN, Jaffar S, Ahmad W (2014). A novel recessive mutation in the gene ELOVL4 causes a neuroichthyotic disorder with variable expressivity. BMC Med Genet 15: 25 4. Raza SI, Muhammad N, Khan S, Ahmad W (2013). A novel missense mutation in the gene FZD6 underlies autosomal recessive nail dysplasia. British Journal of Dermatology 168: 422-425. Abstract Genetic Mapping and Mutation Analysis of Genes Causing Autosomal Recessive Hypotrichosis and Ectodermal Dysplasias XXIX 5. Mehmood S, Raza SI, Younas M, Farhad I , Shahi S, Ayub M, Khan S, Jan A, Ahmad W (2014). Homozygous disease causing mutations in the human hairless gene (Submitted to Iranian Journal of Medical Genetics). 6. Raza SI, Ansar M, Regie LP, Ahmad W, Leal SM (2014). Exome Sequencing identified a disease causing variant in the type I keratin gene KRT25 (In preparation)