حالات کی اک ضرب نے دیکھو
ایسی رُت بھی کھولی تھی
کہ ہم تم جو یکجاں بہت تھے
دور بہت جا بیٹھے تھے
دل کو پر گماں تھا یہ
غلط نہیں ہے تو پر تجھ سے
اس رُت میں غلط ہو رہا ہے
گردش کا یہ کھیل ہے شاید
دل کی ان زمینوں پہ
جو فاصلے بو رہا ہے
اس سب میں تو بھی تھک گئی تھی
چلتے چلتے رُک گئی تھی
میں بھی ٹھیک تھا اپنے تئیں
رُک گیا تھا میں بھی وئیں
پر تجھ سے یوں بچھڑنے کو
میں نے بہتر سمجھا مرنے کو
اُٹھا میں اپنے کمرے بند سے
تیرے پیروں میں انا دھرنے کو
میں آیا سب ٹھیک کرنے کو
The purpose of this research is to see the implementation, evaluation, and advantages and disadvantages of the Tami Otaka Method. The Tami Otaka Method is a method of memorizing by using the ability of the right brain by means of the students memorizing the Qur’an while moving their hands from the verse they are reading. The type of research taken is field research using a qualitative approach. The object of this research is TK PINTAR Bandung, which includes the principal, tahfidz teacher, curriculum, and students. To obtain research data, researcher used observation, interview, and documentation techniques. This research uses descriptive qualitative analysis, so that the results of this study are manifested in the form of words both orally and verbally. The results obtained are as follows: first, to achieve purpose of the learning, TK PINTAR Bandung uses the Tami Otaka method with the help of audio, visual, audio-visual and body movements of a teacher. Second, the curriculum used is a synthesis of the National Curriculum and the Typical Curriculum of TK PINTAR Bandung. Third, learning is carried out in groups. Fourth, the evaluation is given continuously everyday and there is also an evaluation every semester. Tujuan dari penelitian ini adalah untuk mengetahui pelaksanaan, penerapan, evaluasi serta kelebihan dan kekurangan dari Metode Tami Otaka. Metode Tami Otaka adalah Metode menghafal menggunakan kemampuan otak kanan dengan cara siswa menghafal Al-Qur’an sambil menggerakkan tangan dari arti ayat yang dibaca. Jenis penelitian yang diambil adalah penelitian lapangan dengan menggunakan pendekatan kualitatif. Objek penelitian ini adalah lembaga TK PINTAR Bandung yang di dalamnya ada kepala sekolah, guru tahfidz, kurikulum, dan siswa. Untuk memperoleh data penelitian, peneliti menggunakan teknik observasi, wawancara, dan dokumentasi. Penelitian ini menggunakan analisis kualitatif deskriptif, sehingga hasil dari penelitian ini diwujudkan dalam bentuk kata-kata baik tulisan maupun lisan. Hasil penelitian diperoleh sebagai berikut: pertama, untuk mencapai tujuan pembelajaran, TK PINTAR Bandung menggunakan metode Tami Otaka dengan dibantu media auido, visual, audio-visual dan gerakan tubuh dari seorang guru. Kedua, kurikulum yang digunakan sintesis dari kurikulum Nasional dan kurikulum khas TK PINTAR Bandung. Ketiga, pembelajaran dilakukan secara berkelompok. Keempat, evaluasi diberikan terus menerus setiap harinya dan ada juga evaluasi tiap semester.
Alkaloids are produced in plants through various pathways involving several enzymes that lead to diverse alkaloids. One of the most important alkaloid biosynthetic enzymes is coclaurine N-methyltransferase (CNMT) which is an S-adenosyl-L- methionine-dependent methyltransferase (SAM-MTase). SAM-MTases utilize S- adenosyl-L-methionine (SAM) as a cofactor to methylate other molecules. CNMT catalyzes the methylation of coclaurine. Crystal structures of more than hundred SAM-MTases have been investigated. Several O-methyltransferases have been characterized at the molecular as well as structural levels, but there have been very few molecular studies of N-methyltransferases especially about CNMTs. In this study, the amino acids sequence of Aristolochia fimbriata putative CNMT has been determined by isolating and translating the full-length cDNA. In order to investigate the mechanism of methylation by this putative CNMT, three-dimensional homology model has been built and the ligand (SAM) as well as the substrate (S- Coclaurine) has been docked into its active site. Phylogenetic analyses were performed using the MEGA 4.0 software. The phylogenetic relationship of A. fimbriata putative CNMT with their homologs has also been analyzed. In order to identify the putative CNMT gene and determine its function, online similarity searches were performed by BLAST program using the cDNA sequence as well as the putative protein that could be encoded by the gene. All the methods, applied, predicted that the gene identified might be involved in the production of CNMT. The predicted homology model consists of two domains: the N-terminal catalytic core domain and the C-terminal domain. The catalytic core domain has a central sheet of β-strands surrounded by α helices. The catalytic core domain contains binding site for VSAM. The C-terminal domain consists of alpha helices and a few beta sheets creating a pocket for the substrate in between them. The SAM-binding pocket is located next to substrate binding pocket and there is an opening in between these two cavities through which the methyl group of SAM projects towards the substrate. The most important residues involved in the methyl transfer reaction seem to be Tyr-79 and Glu-96.