ضیاء الحسن علوی مرحوم
افسوس کہ میرے رفیق قدیم اور صدیق حمیم مولوی ضیاء الحسن صاحب علوی ندوی نے ایک مختصر علالت کے بعد ۱۴؍ جون ۱۹۴۵ء کو الہ آباد میں جہاں وہ عربی مدرسوں کے انسپکٹر اور مشرقی امتحانوں کے رجسٹرار تھے ستاون برس کی عمر میں وفات پائی، اس حادثہ کی اطلاع مجھے ۱۸؍ جون کو لکھنو میں اسی مدرسہ میں ملی جہاں میں اور مرحوم مل کر ایک جان دو قالب ہوئے تھے، افسوس کہ ایک قالب خالی ہوگیا، اور دوسرا نیم جان موجود ہے، مرحوم مجھ سے عمر میں تقریباً پانچ برس چھوٹے (گو تعلیم کے درجہ میں وہ ایک سال بڑے تھے) اس لئے بظاہر امید یہی تھی کہ انہی کو میری جدائی کا صدمہ برداشت کرنا پڑے گا، مگر تقدیر یہی تھی کہ مجھے ان کے فراق کا غم سہنا پڑے اس لئے امید غلط ثابت ہوئی، اور تقدیر کا فرمان نافذ ہوکر رہا۔
اکنوں چہ تواں کرد کہ تقدیر چنیں بود
مرحوم کا کوری ضلع لکھنؤ کے مشہور علوی خاندان کے چشم و چراغ تھے، دارلعلوم ندوۃ العلماء کے حامیوں بلکہ بانیوں میں رؤساء کا جو طبقہ شامل تھا، ان میں منشی محمد اطہر علی صاحب مرحوم کا نام بہت جلی ہے، یہ خاندان قطب وقت حضرت مولانا شاہ فضل الرحمان صاحب گنج مراد آبادی رحمتہ اﷲ علیہ کا ارادتمند و معتقد تھا، جو ندوہ کی تحریک کے روحانی مرکز و مدار تھے، اس لئے جب ۱۸۹۸ء (۱۳۱۶ھ) میں لکھنو میں ندوہ کا دارالعلوم کھلا تو منشی صاحب مرحوم نے اس درس گاہ کو اپنے سب سے چھوٹے بچے اور ایک ننھے بھتیجے کو نذر کیا، یہی ننھا بھتیجا مولوی ضیاء الحسن صاحب علوی ندوی تھے، دارالعلوم کے طلبہ کے داخلہ میں ان کا نمبر شاید دوسرا تیسرا تھا، عربی کی پوری تعلیم یہیں حاصل کی اور یہیں سے فراغت...
Growth factors are the potential operational members which control different phases of liver regeneration. Different growth factors have expression regulation in the whole process relating to different phases of liver regeneration. Objective: To assess the expression regulation of different growth factors and cytokines involved in liver regeneration in a phase-dependent manner. Methods: Blood and liver samples were collected and analyzed on 1st, 3rd, 5th, 7th and 14th postoperative days after 50% Partia hepatectomy (PHx). Results: Steady increase of liver regeneration rate was recorded from 90.8% (1st day) to 97.9% (7th day). Liver function tests further confirmed the steady liver recovery in PHx mice. Several growth factors such as HGF and VEGF exhibited an up-regulation till 5th day and later gradual decrease till 14th day compared to control mice. Albumin, CK18 and CK19 showed sequential expression increase from 1st to 14th day compared to AFP and HNF-4α upregulated until 5th and 1st day, respectively. Quantification of these growth factors further confirm our results. Conclusions: Conclusively, these results highlight a phase-dependent regulation and role of growth factors in liver regeneration and recovery
A traditional approach to method development could flop to encounter desired separation downstream during test method validation, test method transfer or out of specification studies. In contrast, method development through quality by design (QbD) approach can result in a more rugged/robust method due to greater emphasis on risk management. A design of experiments (DOE) approach which involves both statistical analysis and modeling is used in a QbD approach to understand the impact and interactions between critical method variables. A QbD approach is applied in present study to drug analogs and isomers complex method development using Shimadzu LC Solution Software. The permissible nonconformities of method variables are determined within the design space – the proven acceptable ranges (PARs). The critical method variables in attaining chromatographic resolution for drug analogs and isomers were column chemistry, chromatography type, sample preparation and mobile phase. The prospective intrusion of method variables was determined in terms of desirable method responses, leading to a better method understanding besides achieving anticipated method quality. Effect of column physical properties (length and inner diameter) on separation and speed was investigated during initial screening experiments. Effect of chemical properties (type of surface, pore size and particle size of stationary phase) on sensitivity and retention factor were studied. Both pH and ionic strengths of the aqueous portion of mobile phase were considered in developing rugged methods that were not sensitive to small variations in conditions. Unified quantification methods for structural, functional and direct analogs of sartans (angiotensin II receptor antagonists), statins (3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors), quinolones, proton pump inhibitors (PPI’s) with gastroprokinetic agents and dipeptidyl peptidase-4 (DPP-4) inhibitors with biguanide are developed. Additionally enantiomers of linagliptin, sitagliptin phosphate, cetirizine hydrochloride, solifenacin succinate and montelukast sodium are separated on Diacel Chiralpak IC stationary phase instead of using separate stationary phase for individual enantiomeric separation. The proposed methods were statistically validated in terms of precision, accuracy, linearity, specificity, selectivity and robustness in accordance with guidelines of International Council on Harmonisation (ICH). The newly developed methods proved to be specific, accurate and robust for the unified quantification of drug analogs in commercial pharmaceutical formulations and to confirm the relative abundance of desired enantiomer in a racemic mixture of active pharmaceutical ingredient. The advantage of developed methods for unified quantification of drug analogs is that only one sample is prepared and single chromatographic run is required to provide information on the identity, content uniformity, dissolution, potency and purity of active pharmaceutical ingredients. Therefore, these methods can be handy in daily sample handling in routine, when many samples of drug analogs are analyzed in drug testing laboratories. The proposed methods are able to discriminate not only between different drug analogs and enantiomers but are also able to detect counterfeit products.