موضوع4:تنقید کے اصول
تنقید کے اصول:
تنقید کے اصول اور طریقہ کار میں فرق ہے۔تنقید کے دو مروجہ طریقے ہیں:
• استخراجی طریقہ کار • استقرائی طریقہ کار
استخراجی طریقہ کار:
اس طریقہ کار میں کل سے جزویا عام سے خاص کی طرف جاتے ہیں۔مثلا تمام انسان فانی ہیں لہذابلال بھی فانی ہے۔
استقرائی طریقہ کار:
اس طریقہ کار میں جزو سے کل یا خاص سے عا م کی طرف جایا جاتا ہے۔مثلا خیبر پختونخواہ کے کوے کالے ہیں، پنجاب کے کوے کالے ہیں، بلوچستان کے کوے کالے ہیں لہذاپاکستان بھر کے کوے کالے ہیں۔
تنقید کے اصول
غیر جانبداری:
تنقید کا پہلا اصول غیر جانبداری ہے۔دوستی ،تعلق داری یا پیسے کی لالچ میں جانبداری کا مظاہرہ کرنا نہیں۔اصل نقاد غیر جانبداری کا مظاہر ہ کرتا ہے۔
محنت اور لگن سے کام:
نقاد ہمیشہ محنت سے کام کرتا ہے۔ اچھا نقاد کسی کتاب کو پڑھ کر ہی اس پر تنقید کر سکتا ہے اور رائے دے سکتا ہے۔
حقائق سے آگاہی:
نقاد کو حقیقتوں کا پتہ ہونا چاہیے مثلا انٹرنیٹ کے مسائل ہر جگہ پر ہیں۔ اس حقیقت کو مدنظر رکھ کر رائے دینی چاہیئے یعنی حقیقت پسند ہونا چاہیئے۔
مضمون پر عبور:
ایک نقاد کو اپنے مضمون پر بھرپور عبور ہونا چاہییمثلا نظم پر تنقید کرتے ہوئے نظم کی بنیادی چیزیں مثلا وزن، بحر، ردیف، قافیہ، مطلع، مقطع و?غیرہ کا پتہ ہونا چاہیئے۔ اسی طرح ناول پر تنقید کرنے کے لیے اس کے پلاٹ، موضوع، کردار نگاری،مکالمہ نگاری وغیرہ یعنی اس موضوع کی بنیادیں چیزوں سے آگاہ ہو۔
جرات اظہار:
ایک اچھے نقاد کے اندر جرات اظہارکا ہون ازحد ضروری ہے یعنی مصنف کے قد کاٹھ کو دیکھنے کی بجائے اس کے کام میں جہاں غلطی ہو اسے ببانگ دہل بیان کرسکے۔
شخصیت پرستی سے پرہیز:
نقاد کے لیے لازم ہے کہ وہ شخصیت پرستی سے...
Allah the elevated bestowed on prophet Muhammad SAW two basic sources of guidance for Muslim Ummah, The holy Quran and Hadith. Due to this significance of Hadith, Muslims have invented more than five hundred sciences related to Hadith. One of these sciences is Ilm Garb ul Hadith. Sheikh Moaamer bin muthana was the first scholar who has written a book on this topic. From then on Muslim scholars have researched a lot in this regard. Dozens of scholars spent their time and wealth on it. According to the author of Moaajm ul mua’ajam more than 90 books on the topic have been published but eight of them gained much publicity and famous hood among them. Abu Ubaida, Abu Adnan, Abu Ubaida Qasim bin Salam, Ibrahim bin Ishaq Al Harbi, Abu Ubaida Ahmed bin Muhammad Alhervi, Ibn Jauzi, Muhammad bin Atheer Aljazree, Zemakhsharee. The following article consists of a brief introduction of Ilm Gharib ul Hadith along with a brief history of research about it. Then the eight famous books on Gharib ul Hadith and there way of research are examined in brief along with examples. At last a comparative study of the work done by these eight scholars is given in order to explore the differences and similarities among them.
The present study, deals with the isolation, screening and selection of Aspergillus oryzae for the alpha amylase production. Seventy eight isolates of A. oryzae were isolated from different soil samples. The strains were initially selected qualitatively on starch agar medium and screened quantitatively for enzyme production in shake flasks and a strain producing 130 ±0.1U/ml of enzyme was selected which was assigned the code IIB-30. The selected strain was subjected to physical and chemical mutagenic treatments in order to improve its amylolytic potential. During the treatments, isolates were qualitatively and quantitatively screened. Among these, EMS-18 exhibited the highest enzyme activity (347±1.2 U/ml). This mutant showed 2.6 fold increased activity over the parental strain in terms of enzyme production. The cultural conditions and nutritional requirements of the selected strains (both wild and mutant) were optimized in 250 ml Erlenmeyer flasks prior to scale up studies in a fermenter. Six different fermentation media were evaluated for the alpha amylase production by both wild and mutant strains of A. oryzae in shake flasks fermentation. Of all the media, M4 containing (g/l); starch 20, yeast extract 8.5, NH 4 Cl 1.3, MgSO 4 .7H 2 O 0.12, CaCl 2 0.06 gave maximal enzyme production i.e., 168±2 (wild) and 385±2 (mutant) which was highly significant (p≤0.05). The effect of incubation temperature, initial pH, volume of media and inoculum size was investigated on the enzyme production. The optimal enzyme production was obtained at 30°C, pH 5, volume, 10 % and inoculum size 4 %, by both wild and mutant strains. The rate of fermentation was also studied and the highest yield of enzyme was obtained 72 h after inoculation.xiv Corn starch (2 %) and lactose (1.5 %) as carbon sources while, ammonium sulfate (0.3 %) and peptone (0.2 %) as nitrogen sources were also optimized. Different surfactants were added to the fermentation media and Tween 80 at the level of 0.1% was found to be the best for enzyme production. The scale up studies for alpha amylase production was carried out in a 7.5 L stirred fermenter. The rate of fermentation for enzyme production by both wild and mutant strains was investigated.) It was found that the enzyme production increased gradually and reached maximum (335 U/ml and 608 U/ml) after 64 h (wild) and 48 h (mutant). The kinetic depiction of results showed optimal fermentation period for enzyme production to be 64 h and 48 h, respectively. The other cultural conditions such as initial pH (5), aeration level (1.5 vvm), dissolved oxygen (15 %), inoculum size (10 %) and agitation intensity (200 rpm) were optimized for enzyme production. The fermented broth was subjected to ammonium sulfate precipitation at different saturation levels (20-90 %). The optimum level of ammonium sulfate saturation was found to be 70 % that gave 1.3 fold purification. By using Sephadex- DEAE column, the active fractions were eluted using 0.05 M Tris-HCl buffer containing 0.30 M NaCl at pH 7.5. The molecular weight of alpha amylase was found to be 48 kDa on SDS-PAGE after gel filtration. A total of 9.5 fold enzyme purification was accomplished. The effect of time, temperature, pH and metal ions on purified enzyme was also investigated and maximum activity was achieved after 30 min at 40oC and pH 5 in the presence of Ca+ 2 ion.