سائنس اور تہذیب و تمدن کے فروغ میں اسلام کا کردار

Islam is a complete code of life. It has complete instructions regarding all aspects of life. There is no moment in human life when the wisdom of Islam has not benefited humanity. Likewise there is no such area in the world where Islam has not put the light of guidance. Allah Almighty has systematically administered this world system.  Allah created the universe and set it disciplined, then created man and gave him every kind of physical, rational, logical and argumintive ability, so that humans can know the secrets of the universe by thinking and understanding it. The journey of research and discovery, which started long ago, is still continues today after passing through different stages of research. This journey of research resulted in evolution of philosophy, civilization, promotion of new scientific innovations and discoveries. The research and discovery resulted in the secrets of heaven and earth.  Today, man stands at the stage of science and technology, about which the human being in the older days even could not imagine of it. In this entire evolutionary journey, the religion of Islam is the only religion that not only stood firmly with humans, but guided him at every step during this evolutionary journey. In this context, the Quranic teachings are comprehensive matter. Allamah Sayyuti says "There is no knowledge and problem that is not discussed in the Qur'an. So Islam invites human to research in the system of the universe and presents the system of universe for its authenticity.

Diagnostic Potential of Indigenously Hyperproduced Urate Oxidase

The main purpose of this research work was to optimize the production of urate oxidase through mutagensis of Bacillus subtilis. The organism was subjected to ultra violet irradiation and chemical mutagenesis. Ethyle methane sulfonate treated B. subtilis (180 minutes) was proved to be the best for optimum production of urate oxidase by 3 log kill/survival curve. Fermentation medium was also optimized, it was found that substrate concentration (0.5%), fermentation period (36 h), pH (8.5), temperature (35 oC), yeast extract (0.3%) and sucrose (2%) enhanced the activity of the parent and mutant derived enzyme. The enzyme was purified by adopting different techniques i.e ammonium sulfate precipitation, ion exchange and gel filtration chromatography. It was observed that mutated enzyme exhibited 97.56 U/mg specific activity with 256.73 fold improvement. The purified urate oxidase was run on SDS-PAGE which determined a single band with molecular weight of 34 kDa. The purified BSM-2 possessed Km and V max value 0.067 M and 133.3 IU mg-1 min-1 respectively. The optimum pH and temperature for catalytic activity were 7.5 and 35oC respectively. The activation energy for formation of ES complex was 43.4 kJ/mol. Enthalpy (ΔH*), entropy of activation and Gibbs free energy demands for urate oxidase inactivation were 30.26 kJ/mol, -106.27 J mol-1 K-1, 62.99 kJ/mol respectively. Barium chloride, potassium cyanide and zinc sulfate decreased the activity of the enzyme. Whereas, sodium chloride (0.6M), potassium chloride (0.4M) and calcium chloride (0.4M) enhanced the enzymatic activity 123%, 117% and 119% respectively.