بین المذاہب ہم آہنگی اور عصر ی تقاضے : الہامی تعلیمات کی روشنی میں تجزیاتی مطالعہ

Inter-Faith Harmony and Contemporary Demands: An Analytical Study in the Light of Divine Teachings Abstract Over the course of time and with the rapid increase in human population need for mutual relations become crucial. Resultantly on behalf of this closeness, separation, anti-standpoints and comparisons also emerged. As the time passed by hatred and hypocrisy and other social vices spread on large scale. Thus human society was waiting for such liberator who may lead and work for the betterment of this society. With the dawn of Islamic civilization all such issues were not only resolved but also provided with a model for containing the difference of opinion and multiple traditions under its unique worldview. Islamic History presents itself as a model where the minorities were provided with the opportunities of participating in political, social, educational and collective affairs. Thus in a society where tyranny, injustice, un-forbearance, religious intensity, terrorism and the activities of violating the human rights were very common, were substituted by the Islamic ideal  of forbearance. It is argued here that the solution of all these issues was only in religion contrary to what is being claimed about an idea of social harmony where religion is not given its due position. Today it’s our dire need to develop a sense of harmony, modesty, affection and peacefulness among the masses of various religions of Pakistani society. It is further argued that for this very noble cause all the religious scholars and their followers can come forward playing their pertinent role. Keywords: Interfaith harmony, present era, divine books, religious personalities, Peace

Genetic Analysis of Autosomal Recessive Intellectual Disability in Consanguineous Families

Intellectual disability (ID) is a common and highly heterogeneous neurodevelopmental disorder. It affects 1–3% of the world’s population and its prevalence is almost twice as high in underdeveloped than in affluent world. ID has an enormous socio-economic burden and devastating impact on the lives of affected individuals and their families. More than 700 genes have been discovered so far across different studies pertaining to X-linked, autosomal dominant and recessive ID. Autosomal recessive intellectual disability (ARID), being the most common form of ID. Till date, more than 577 genes involved in ARID have been discovered mostly in consanguineous communities. ARID is further grouped into syndromic ARID (nARID) and non-syndromic ARID (nsARID) types primarily based upon the phenotypic appearance. In Pakistan, the rate of consanguinity is approaching 70% and this high rate results in higher rates of recessive disorders including ARID. Inbred families provide a unique opportunity to find pathogenic variants in known as well as candidate genes responsible for recessive disorders due to the extensive regions of homozygosity in the genomes of these individuals. We enrolled 25 suspected consanguineous ARID families based on pedigree analysis and clinical evaluation from Kohat district, KPK province of Pakistan. The identified consanguineous families were subjected to genetic analysis through Whole exome sequencing (WES) and genome-wide SNP array. Bioinformatics analysis was carried out using Genome Analysis Tool Kit (GATK) based bioinformatics pipeline and Consanguinity Analysis Through Common Homozygosity (CATCH) software. Subsequent prioritization was carried out by application of various quality filters. Finally, the selected putative causative variants were validated by Sanger sequencing. Out of 25 families, known causative mutations in five known genes (ASPM, ATRX, GPR56, NAGLU and DOLK) were identified in eight different families for ARID. Furthermore, novel mutations in seven known ID genes (ABAT, SLC12A6, SHANK3, BCKDK, DDHD2, ERCC2 and GPT2) were found in seven families. Three potential likely pathogenic mutations were identified in three different families in three novel genes (DYNC1I2, FBXL3 and LINGO1). However, seven families remained unsolved; probably we missed the causative variants. Our study showed that v exome sequencing in combination with microarray genotyping is a powerful technique to find out the causative variants in rare ARID families and can be used to establish correlation between candidate genes and phenotypes. The diagnostic yield of the strategy used in our whole ID project was found to be approximately 32% (32/100). Furthermore, the present work also revealed that the genetic variations are associated with a significant number of consanguineous ARID families from Pakistan.