مولانا عبدالشکور
ہمارے پرانے نامور علماء ایک ایک کرکے، اٹھتے جاتے ہیں، افسوس ہے کہ ان کی آخری یادگار مولانا عبدالشکور صاحب نے بھی سفر آخرت اختیار کیا، مولانا کی ذات جامع کمالات اور اس دور میں سلف صالحین کا نمونہ تھی، علم و عمل اور دین و تقویٰ میں ان کا درجہ بہت بلند تھا، تعلیم و تدریس، تالیف و تصنیف، وعظ و تبلیغ، ارشاد و ہدایت ہر راہ میں ان کے نمایاں کارنامے ہیں، تقریباً نصف صدی تک ان کا فیض جاری رہا، اور ان کے ذریعہ بہتوں کو ہدایت حاصل ہوئی، ایک زمانہ میں پورے ہندوستان میں ان کے کارناموں کی شہرت تھی، مگر ادھر پچیس تیس سال سے انھوں نے خاموشی اور گوشی نشینی کی زندگی اختیار کرلی تھی، اور موتو اقبل ان تموتوا کی عملی تفسیر بن گئے تھے، اب ایسے ربانی علماء کا پیدا ہونا مشکل ہے، اﷲ تعالیٰ ان کے خدمات کو قبول اور ان کے مدارج بلند فرمائے۔ (شاہ معین الدین ندوی، جنوری ۱۹۶۲ء)
The research methodology represents a scientific feature that highlights its manifestations and its fruits according to the nature and results of the methodology; this shows the intellectual contribution and impact on the advancement of human civilization in the scientific field. The methodology of Muhaddithin provided an innovative and practical approach about the certainty of narations which they had devised and applied. The question is: what is the way to document the narrations? To answer this question needs to refer to the books of sciences of Hadith, and when looking at these books shows that they are addressing this issue under different topics. But the knowledge of narrations in terms of the factors pertaining to certainty is not covered in special sub title, but there are hints scattered in different places in the books of sciences of Hadith. There is still a need to address the issue comprehensively in all aspects relating the factors of certainty, Hence the goal of the research from the next two points 1- Exploration the foundations and factors pertaining to the certainty about the narrations. 2- Highlight the methodological innovation according to Muhaddithin in this regard. The purpose of this article is to provide a comprehensive overview of Methodological Bases and warranting Circumstances for Sunnah Authentication.
IN VITRO CHARACTERIZATION OF PROBIOTIC PROPERTIES AND ANTICAMPYLOBACTER ACTIVITY OF LACTOBACILLI ISOLATED FROM POULTRY The aim of the present study was to isolate, characterize and develop indigenous probiotic lactobacilli targeting mitigation of Campylobacter jejuni. Lactobacilli (n=150) were isolated from poultry (62), fermented foods (78) and human faeces (10).All isolates were preliminarily screened for their anticampylobacter activity by well diffusion assay. Sixteen pre-selected isolates had activity against Campylobacter jejuni (n=4). Cell free supernatants (CFSs) of eight isolates (PL22, PL53, PL88, PL120, PL135, PL141, PL145 and PL149) retained activity after pH adjustment (6.5), while CFS of seven isolates (PL22, PL53, PL120, PL135, PL141, PL145 and PL149) retained activity after boiling at 80°C for 10 minutes. Selected isolates were further characterized for their tolerance to low pH and bile salts, antibiotic susceptibility, auto-aggregation and co-aggregation properties. All selected isolates had significant tolerance to low pH (>50% survival at pH 3), bile salts (0.3%), autoaggregation and coaggregation properties. Selected isolates were safe and had no acquired resistance to penicillin, tetracycline, ampicillin and erythromycin. PL53, PL120 and PL149 showed significantly higher reduction (> 3 log reduction) of Campylobacter jejuni ATCC 33291 in broth culture. It is concluded that Lactobacillus gallinarum PL 53, Lactobacillus casei PL 120 and Lactobacillus gallinarum PL 149 had probiotic potential and anti-campylobacter properties.Keywords: probiotics, Lactobacillus, Campylobacter, pH tolerance, antimicrobial activity EFFECT OF NEWLY CHARACTERIZED PROBIOTIC LACTOBACILLI ON WEIGHT GAIN, IMMUNOMODULATION AND GUT MICROBIOTA OF CAMPYLOBACTER JEJUNI CHALLENGED BROILER CHICKEN The aim of the present study was to determine the competitive exclusion of Campylobacter jejuni inpoultry gut by three potential probiotic lactobacilli strains. A total of 135 birds of one day age were randomly divided into nine groups. The groups of prevention model received respective strains from day 1-35 and treatment model received lactobacilli from day 15-35 (after challenging with C. jejuni). These groups were given lactobacilli (~108 CFUs) while challenged with C. jejuni on day 14 by oral gavage (106 CFUs/bird). There were three control groups including A, B and I. Negative control (A) did not receive any treatment, C. jejuni was given to group B and group I was given Enrofloxacin formulation. Cloacal swabs were collected from birds of each group before and after challenge while the ceca were collected from birds after slaughtering (on day 35) for enumeration of aerobic bacteria, coliform, C. jejuni, lactobacilli and Bifidobacterium on selective agar plates. Effect of lactobacilli on weight gain and New Castle Disease vaccine (NDV) titer were also evaluated. Probiotic strain Lactobacillus gallinarum PL 53 considerably decreased log10 values of aerobic plate count (3.19±0.66), coliform count (2.83±0.22) and C. jejuni (3.98±0.77) in poultry. The probiotics also enhanced Lactobacillus and Bifidobacterium spp. counts (~2-3 log increase). Treatment group C had maximum weight gain (1994±188.32g) and geometric mean titer (274.4) on day 28. The results of in-vivo experiments concluded that probiotic administration may be effective for targeted mitigation of C. jejuni in broiler birds.