پروفیسر افغان اﷲ
یہ خبر افسوس ناک ہے کہ اردو کے ایک اور خدمت گزار پروفیسر افغان اﷲ خاں صاحب نے بھی اچانک آخرت کا رخت سفر باندھ لیا، ایک سمینار میں شرکت کے لیے انہوں نے دہلی کا سفر کیا تھا لیکن کیا خبر تھی کہ یہ اس دنیا کے آخری سفر کی تیاری تھی، سمینار کے پہلے روز وہ پورے نشاط کے ساتھ مختلف نشستوں میں شریک رہے، دوسرے روز دل میں درد اٹھا جس نے دنیا کے ہر دکھ سے ان کو نجات دے دی، وہ گورکھ پور یونیورسٹی کے شعبہ اردو کے صدر تھے، سکونت بھی اسی شہر میں تھی لیکن رہنے والے اصلاً وہ اعظم گڑھ کے ایک گاؤں خالص پور کے تھے شروع سے ذہین تھے، فراق گورکھ پوری پر جناب محمود الٰہی کے زیر نگرانی پی ایچ ڈی کی سند حاصل کی اور اس شان سے کہ موضوع پر یہ مقالہ خود سند بن گیا، کئی کتابیں سپرد قلم کیں، تاریخ ہند سے متعلق طراز ظہیری ان کی آخری تالیف تھی، باغ و بہار شخصیت کے مالک تھے، دارالمصنفین سے رشتہ اخلاص تھا، آخری بار وہ علامہ شبلی سمینار میں شرکت کی غرض سے یہاں آئے تھے، اتفاق ہے کہ مارچ کے معارف میں ان کا مضمون شائع ہوا، وہ ایک ہمدرد استاد، ہمدرد دوست اور ہمدرد انسان تھے، یو پی اردو اکیڈمی بھی ان کی سرگرمیوں کا مرکز تھی، گوشہ نشین اردو کے خادموں کی خدمت انہوں نے بے غرض ہو کر کی، صرف ۵۷ سال کی عمر میں ان کا اس طرح رخصت ہوجانا اردو کے لیے ہی نہیں، انسانیت اور شرافت کی دنیا کے لیے ایک بڑا حادثہ اور خسارہ ہے، خدا مغفرت فرمائے، آمین۔ ( عمیر الصدیق دریابادی ندوی ، اپریل ۲۰۰۸ء)
The term “Balãgha” is used in Arabic literature. The word Balagha is derived from a root “ بلغ” (Balãgha) meaning “ to reach” and the etymology is explained by interpreting the “Balãgha” as the art of reaching the listener in attempting to convey one`s idea to him, or the art of reaching the utmost perfection in the style and content of a composition. A perfect word for the term Balagha in English literature is Rhetoric. It is the body of principles and theory having to do with the presentation of facts and ideas in clear, convincing and attractive language. The traditional aim of rhetoric was to give effectiveness to public speech. Rhetoric is a comprehensive science just as much concerned with what one could say as how one might say it. It studies the effectiveness of language comprehensively including its emotional impact as well as its propositional content. The title of my paper reflects the rhetorical aspects of the Sūrah Al-Rahmān. It is a part of the Holy Quran.
Economical production of industrial enzymes in higher yields has been an area of intensive research interest for the last many decades. In recent years, scientists have made tremendous progress in enzyme biotechnology that has opened up new opportunities for enzymes in molecular biology as well as industrial applications. Fungi belong to a group of organisms with the ability to produce different types of enzymes. The importance of fungi is not limited to their native products; they are also useful in the development and commercialization of new products through the modern techniques of mutagenesis and molecular biology. Enzyme production in higher yields is important for any biomedical or industrial application of enzymes. White rot fungi are efficient lignin degrading microorganisms that produce high activities of ligninolytic enzymes, including lignin peroxidase, manganese peroxidase and laccase. Manganese peroxidase has got tremendous applications in biomass utilization, bioremediation, pulp and paper industry, food technology, nanobiotechnology and medicine. With increasing new biotechnological and industrial applications of manganese peroxidase there is dire need to search for new and hyperproduccing enzyme sources. The various techniques for developing hyperproducing fungal strains are radiation and chemical mutagenesis, and genetic engineering. Chemical mutagenesis offers the possibility of a wider and more economical technique for developing mutant strains for producing biocatalysts in industry, waste treatment, medicine, and in the development of bioprocess monitoring devices like biosensors. Keeping in view the above mentioned facts, a study was conducted to develop a hyperproducing mutants of Coriolus versicolor IBL-04 through chemical mutagenesis. Two chemical mutagens ethedium bromide (EB) and ethyle methansuphonate (EMS) were used for mutational work and hyperprucing mutants in each case were selected on basis of MnP production in solid state fermentation. The physical and nutritional parameters for the production of MnP by native and selected mutants were optimized through one-factor-at-a-time optimization stratergy. Physico-chemical culture conditions, such as pH, tempature, moisture levels, inoculum size, C:N ratio, mediators and metal ions were optimized and maximum MnP activities by mutant strains EB-60 and EMS-90 were 2796 U mL -1 and 3140 U mL -1 , repectivelty, as compared to 1635 U mL -1 MnP actvity from native strain. An increase of 3.2-fold in enzyme production after mutagenesis and optimization of various parameters was achieved as compared to native strain. The MnPs produced by native and mutant strains were 1.4-5.5 fold purified through ammonium sulphate precipitation, ion exchangechromatography and gel filtration. The purified MnPs from from mutant strains had the molecular masses of around 43 kDa on SDS-PAGE. The enzymes from native , EB-60 mutant and EMS-90 mutant strains of Coriolus versicolor were compared in terms of their pH & temperature optima and kinetic characteristics. The lower K M and higher V max values from selected mutant strains suggested that the MnPs from mutants were more efficient and stable as compared to MnP from the native strain. The results of this work demostrated that the random chemical mutagenesis of Coriolus versicolor IBL-04 significantly enhanced MnP production under optimized laboratory conditions. Higher activities and thermo-stabilities of MnPs from mutant strains suggest the potential of mutant strains for commercial scale MnP production for diverse industrial applications.