دین کی اصل توحید ہے قرآن کے مطلوب انسان کی زندگی ایک اللہ کی وفادار ہوتی ہے اس کا ہر سجدہ ،ہر امید، ہر اندیشہ، ہر محبت، ہر خوف، ہر دعا ،ہر عبادت صرف اور صرف اللہ کے لئے ہوتی ہے وہ اپنے رب کے ساتھ کسی کو شریک کرنے کا تصور بھی نہیں کر سکتا اللہ کے سوا کوئی رب نہیں ہے وہ واحد و یکتا ہے وہی معبود برحق ہے ۔
اللہ تبارک وتعالیٰ قرآن مجید میں ارشاد فرماتے ہیں:
" اِنَّ اِلٰهَكُم لَوَاحِدٌ "۔[[1]]
"بیشک الہ تم سب کا ایک ہے"۔
قرآن میں بہت سے مقامات پر ہمیں توحید کا بیان ملتا ہے۔اس آیت میں الہ کی بنیادی نوعیت یہ ہے کہ معبود حقیقی سب کا ایک ہی ہے۔ اس میں تعدد کا احتمال نہیں ہے یہ خدائی اور معبودی ہے نہ اس کے سوا کسی کو معبود بنا سکتے ہیں اور نہ ہی کسی اور سے خیر کی توقع کی جا سکتی ہے۔ یہ توحید ہی سب سے پہلی اور سب سے بڑی چیز ہے جو ملت ابراہیم علیہ السلام کی وراثت کی حیثیت سے اس امت مسلمہ کی طرف منتقل ہوئی ہے ۔ اللہ تعالیٰ اپنی ذات و صفات میں یکتاو یگانہ ہے کوئی اس کی برابری کرنے والا نہیں ہے کوئی اسکا ہمسر مسلمان ہونے کے لیے ان تمام عقائد پر ایمان لانا ضروری ہے جن کو ہمیں قرآن میں حکم دیا گیا ہے۔
ارکان"رکن" کی جمع ہے اور رکن کسی بھی چیز کا اہم جز ہوتا ہے جس کے بغیر وہ مکمل نہیں ہوسکتی ایمان کے چھ ارکان ہیں لہذا اگر ایمان کا ایک رکن بھی ساقط ہو جائے تو انسان مومن نہیں رہتا خواہ وہ لاکھ ایمان کے دعوے کرتا رہے جیسے...
Qur’ān is the Words of Allah (SWT). Its interpretation is very difficult job because of the concept that how one can understand the will of Creator. Prophet Muhammad (SAW) was the first exegete of Qur’ān. His companions were the next one. This chain is continuously running till now. In subcontinent, translations of Qur’ān and its exegesis work started in third century Hijrah. Hundreds and Thousands of Qur’ānic exegeses exist in subcontinent in Arabic, Persian, English and Urdu languages. In subcontinent Sir Syed, Modūdī, Farahī, Shabir Uthmānī, are the big names of the field. Everyone has chosen a secluded methodology/principle to interpret the Qur’ān. These principles are known as Usūl-e-Tafsīr. There are many differences among these Usūl, due to personnel mindset and social scenario of different era. The questions that why much diversity exists in these and what are its causes, are being addressed here in this article. On the basis of analytical study, it is found that reason behind this diversity is the concept that exegesis of Quran is based on verbal traditions instead intellectual. Secondly, no one compiled these principles/methods for interpretation of Qur’ān in early centuries. In ninetieth century, due to the challenge of science and Orientalism, some scholars compiled Usūl-e-Tafsīr according to their own understanding and some insisted on traditional continuity.
Polyhydroalkanoates (PHAs) are polyesters that are synthesized by different microorganism and stored as granules in microbes. They are used as a food under starvation. Studies have shown that physical properties of different PHAs resemble significantly to the petroleum based plastics and polyethylene. Additionally, microbial PHAs can be spun, mould and converted into filaments. On the other hand, PHAs are biodegradable material. Such promising features make PHAs as a potential candidate for next generation plastic as it will reduce the pollution caused by the petroleum based plastics. However, PHAs are costly as compared to the different petroleum based plastics. Therefore, researchers are trying to identify new microbes that may produce high amount of PHAs from waste carbon sources. They can accumulate high concentration of PHAs. During the crushing season sugar industries are producing tones of organic waste, specifically they are draining a considerable amount of sugar in canals. This can be a source of food for different types of bacteria, specifically for PHA accumulating bacteria. In this study, different PHAs accumulating bacteria were identified from the swage of sugar industry waste. Five soil and sewage samples were collected and stored at room temperature. For isolation and identification of PHAs accumulating bacteria, Samples were allowed to grow on LB agar medium plates supplemented with 1% Nile blue A. Different concentrations of Nile blue A were used for different incubation periods and colonies were screened. All these colonies were observed under ultraviolet light; PHAs accumulating bacteria were showed bright orange color inflorescence while non PHAs accumulating bacteria appeared white. The PHAs accumulating bacteria were selected and then amplified for 16S rRNA gene sequencing through PCR. In this study we have identified five PHAs accumulating bacteria from the waste of sugarcane industry by using the chemical and sequence analysis. These bacteria can be used for the laboratory and large scale production of different types of PHAs.