حضرت شیخ الحدیث مولانا محمد زکریارحمۃ اﷲ علیہ
حوادث اور تھے پر دل کا جانا
عجب اک سانحہ سا ہوگیا ہے
واحسرتا!آخر۲۴/مئی کوحضرت شیخ الحدیث مولانا محمد زکریا بھی ہم سے جداہوکر قرب وجوار خداوندی کے اپنے اس مسکن حقیقی میں پہنچ گئے جہاں جانے کے لیے جیسا کہ آپ کا ہر ندیم وہم نشین محسوس کرسکتا تھا، برسوں سے آپ کی روح پرفتوح مضطرب وبے قرار تھی اور عالم اسلام گوہر شب چراغ ملّت بیضا سے یکسر محروم ہوگیا۔ اناﷲ واناالیہ راجعون۔
گذشتہ سال حضرت شیخ نے رمضان المبارک کاپورامہینہ جنوبی افریقہ کے ایک مقام اسٹرنگر میں گزارا تھا، حسن اتفاق سے اپنے ایک عزیزدوست مسٹر موسیٰ پارک کی نجی دعوت پر راقم الحروف بھی رمضان کے آخری ہفتہ میں ڈربن پہنچا، ڈربن سے اسٹرنگر کافاصلہ ڈیڑھ سو کلومیٹر ہے جوجنوبی افریقہ کی زندگی میں درحقیقت کوئی فاصلہ ہی نہیں، اس لیے جب تک حضرت کا قیام اسٹرنگر میں رہا تو وہاں اوراس کے بعد جب آپ ڈربن اوراس کے اطراف واکناف میں ایک ایک دودودن کے لیے قیام فرماہوئے توان مقامات پروقتاً وقتاً خدمت سامی میں برابر حاضر ہوتارہا، اس اثنا میں میں نے حضرت کی صحت اور نقل وحرکت سے مجبوری ومعذوری کاجوحال دیکھا اورساتھ ہی یہ بھی دیکھا کہ حضرت کاپروگرام جنوبی افریقہ،مزمبیق اورنیروبی کامکمل دورہ کرنے اور اس کے بعدانگلینڈ جانے کا ہے اورپھریہ مشاہدہ بھی کیا کہ جہاں کہیں پہنچتے ہیں ہرطبقہ اورہرگروہ کے ہزاروں بوڑھے اورجوان پروانوں کی طرح آپ پرگرتے ہیں توان سب چیزوں کے پیش نظر معاً خیال ہوا کہ یہ جوکچھ ہورہاہے کسی غیبی حکم اوراشارہ پرہورہاہے اور ساتھ ہی اندیشہ اس بات کا ہوا کہ غالباً اب وہ وقت قریب ہے جب کہ یاایتھا النفس المطمئنۃ ارجعی الی ربک راضیۃ مرضیۃ صدائے غیب حضرت کے سامعہ نوازہوگی، بعد میں یہ خیال اوراندیشہ دونوں صحیح ثابت ہوئے،...
It is with profound pleasure that we write this editorial to welcome you to the new journal, “Pakistan Biomedical Journal” (PBMJ), an interdisciplinary international journal. PBMJ has successfully completed its first volume and now its the second volume. We greatly appreciate the response of scientists who have contributed previously and are still contributing to this new journal. The subject of the journal is interesting and we try to address the health related concerns of public and improve the understandingof scientific phenomenons by researchers. Research discoveries are happening at a fast pace, in all the fields and PBMJ provides an ideal forum for exchange of scientific knowledge in terms of full length papers, surveys, reviews, case studies, letters to editor and systematic analysis. PBMJ is committed to publishing all manuscripts receiving a high recommendation from reviewers. The intention of PBMJ is to create space for generation of new knowledge, debate, collaborations among national and international scientists. Our vision is to promote research that will be helpful for knowledge sharing, new discoveries, development of critical thinking among the upcoming scholars, guidance for policy makers, awareness among the concerned community and ultimately benefitting the general population in improving health and fitness at large. It is a matter of pride for us to haveexcellent editorial board members from renowned institutes. We aim to have the best standards of quality of the published manuscripts. With every issue, we are continuously trying to improve the standards. We look forward for more exciting researches and scientific studies from all over the world. We would like to extend a very warm welcome to the readers of PBMJ and hope you will join us as authors, reviewers and editors in future.
The present study was conducted on different accessions of Moraceae to evaluate the genetic variation in them on the basis of morphological and biochemical attributes as well as by using molecular markers (SSR and ISSR). The developmental genes cup shaped cotyledons (CUC2) were also studied in order to confirm their functional status. For morphological, biochemical and molecular studies mulberry fresh leaves were taken and all the parameters were taken in replicates. Morphologically leaves of the majority of the accessions had light green color, acute tip, and soft surface along with cordate shaped leaves.The others had lobed shape, dark color, doubly serrate or dentate, acuminate and coarse leaves. There were significant differences in means of lamina length, lamina width and leaf area index. In biochemical analysis, the carbohydrates content was the highest in Korean subni (3.7626±0.204 cm) followed by Morus nigra(3.728±0.1795 cm), Morus latefolia (3.643±0.2458 cm) and P.F.I (3.568±0.3057 cm). Punjab II (1.704±0.1695 cm) showed the lowest carbohydrates content. The protein contents of Kanmasi Japan (1.7007±0.0049 cm) were higher as compared to Punjab-II-punjab (1.4030±0.0455 cm). The higher values of carbohydrate and protein content revealed that the leaves had the more palatability for silkworms. Lun-40-punjab showed the highest total phenolic content. Phenolic play a key role in traditional medicines. The maximum IC50 value was of Morus indica(172.21 mg/g) followed by Morus alba(152.7 mg/g) and PFI (122.4 mg/g). Punjab II Punjab (11.6), Kanmasi Japan (11.5) showed the minimum and same amount of anti-oxidant activity. One of the developmental genes CUC2 was observed in seven Morus strains. This strategy produced good-quality sequences, which were analyzed and compared to each other and to theCUC2c sequence of the reference M. notabilis strain.The sequencing results showed that the mean distance present in the overall sequence pairs xvii was 0.017. Molecular diversity between thirty accessions was checked through DNA markers i.e. SSR and ISSRs by the use of different softwares (NTSYS PC 2.2, Minitab, Statista, R) The SSRs produced 15 polymorphic bands out of 25 (60%) SS18 and MULSTR1 showed best results with the highest PIC and hundred percent polymorphism while the least was shown by SS04. It was also obvious that pic of SSR was somehow less than ISSR. The analysis of molecular variance (AMOVA) gave P value of 0.6434. The ISSR markers showed more polymorphism with more PIC value. The P value obtained from the results was 0.1089, while CV was 35.7. Our diversity results proved that all group were somewhat distant