چاند مدھم ہے، روشنی کم ہے
خواب دھندلے ہیں، آنکھ بھی نم ہے
عمر حاضر جواں دلوں کا کھیل
عمرِ رفتہ عجیب سا غم ہے
زندگانی نثار تم پہ کروں
وار دوں تم پہ سب مگر کم ہے
زندگی! تیرے آستانے پر
موت آنے سے ایک ماتم ہے
دل بھی چاہے کہ زخم تازہ رہیں
وقت کی راگنی بھی مدھم ہے
جس قدر خواہشیں ہیں سینے میں
خضر کی عمر بھی ہمیں کم ہے
آئو ہم بھی گزار لیں لمحے
سانس رکتی ہے آخری دم ہے
تم فضاؔ ہو جہاں پہ چھا جائو
ہم دھواں ہیں ہمیں یہی غم ہے
Due to the significance of Sīrah, Muslim scholars started writing about it from the very beginning of Islam. Pashto language, like other major languages, contains the Sīrah writings. There are two types of Sīrah writings in Pashto language. The first type of books are written for a common man, in a very simple language and style, just to get guidance from the Sīrah of the Holy Prophet (P.B.U.H). This type of books were discussed by Dr. Sa’īdullah Qaḍī in his article “Sīrah books in Pashto Language” published in “Al-Ḥaq” Monthly Journal of Jami’a Dārul Uloom Ḥaqqānia, Akora Khattak. The second type of Sīrah books are written after deep research and criticism of the narrations. But it is worth mentioning that no comprehensive research has been carried out so far which can cover both types of books. Therefore an analysis of the Selected Sīrah writings in Pashto Language has been made, to explore the remarkable Sīrah writings in Pashto Language.
Organophosphate pesticides are of common interest due to increased envionmenal contamination. Such agents cause several undesired and un-solved problems in non-target species. The enzyme organophosphate hydrolase (OPH) producing bacterial strain Brevibacillus parabrevis SR2729 (B. parabrevis SR2729) was isolated from soil contaminated with chlorpyrifos. Brevibacillus parabrevis SR2729 was subjected to strain improvement by physical and chemical mutagenesis; where two strains B. parabrevis SR2729-A and B. parabrevis SR2729-B were selected after classical screening through Triton-x-100 and 3,5,6-trichloro-2-pyridinol. The mutant derived strains Brevibacillus parabrevis SR2729-A and Brevibacillus parabrevis SR2729-B showed 229 and 240 % greater OPH production as compared to parental strain. The optimum levels of six variables (pH, temperature, incubation time, carbon source, nitrogen source and pesticide concentration) were obtained after statistical analysis by RSM. After process optimization, the activities of OPH from B. parabrevis SR2729-A and B. parabrevis SR2729-B were 195 and 178 % greater than OPH from parental strain B. parabrevis SR2729. The specific activity for purified enzyme was observed to be 935, 1521 1911 U mg-1 from parental and mutant derived strains B. parabrevis SR2729-A and B. parabrevis SR2729-B, respectively. After kinetic and thermodynamic analysis, the enzyme from mutant derived strains was observed to be more suitable for utilizing chlorpyrifos as substrate, with higher thermostability as compared to the enzyme from parental strain. The molecular weight of OPH enzymes from parental and mutant derived strains were 37 kDa. In soil, the OPH from mutant derived strains B. parabrevis SR2729-A and B. parabrevis SR2729-B showed 206 and 208 % greater degradation of chlorpyrifos as compared to parental strain. The enzyme from mutant derived strains B. parabrevis SR2729-A and B. parabrevis SR2729-B degraded 28 and 65 % greater insecticide in water compared to enzyme from parental strain. In conclusion, the strain improvement by physical and chemical mutagenesis resulted in the selection of potential mutant derived strains which were more significant agents for the degradation of chlorpyrifos in soil and water samples.