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A Study of Candidate Genes Involved in Inherited Alopecias

Thesis Info

Author

Muhammad Hassan Ullah

Department

Deptt. of Biochemistry, QAU.

Program

Mphil

Institute

Quaid-i-Azam University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2010

Thesis Completion Status

Completed

Page

59

Subject

Biochemistry

Language

English

Other

Call No: DISS/M.PhIl BIO/2587

Added

2021-02-17 19:49:13

Modified

2023-02-19 12:33:56

ARI ID

1676717238568

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 کانت شاعرتنا إنسانۃ لطیفۃ للغایۃ وکانت متعلمۃ وقد حصلت علی الشھادات العالیۃ وعلی وظائف رائعۃ ومع ھذا کانت إنسانۃ لا تنسیٰ الجمیل. یقول عنھا أحمد ندیم قاسمي أنہ رأی الکثیر من الشابات والشباب الذین تقدموا إلی الأمام وقد حصلوا علی مراتب عالية ولکن لم یذکروا أسماء أساتذتھم وکبارھم، إنما بروین فکانت کلما تکلّمت ذکرت اسم احمد ندیم قاسمي مع الذین استفادت منھم ومنحوھا بعض النصائح والخبرات فھي کانت صاحبۃ الخیال والقلب الواسع[2]۔

 کانت الشاعرۃ صاحبۃ الخیال الواسع ذات ھمۃ وعزیمۃ قویۃ، لا تتردد إذا قررت فعل شیء، ولا تتراجع عن الحق ولا تھتم بالأمور التافھۃ، إنما کانت تواجہ المشاکل بکل جرأۃ وھمّۃ وکانت صاحبۃ العقل والفھم السلیم، ولکنھا في نفس الوقت تحملت المصاعب والمشاکل بکل جرأ ۃ...

السلم و السلام و تطبيقا تھا في ضوء السيرة النبوية

There is no doubt that Islam is a religion of peace which ensures peace regionally and globally in the light of its teachings. It is not only a religion of worship, but it is a religion includes ethics and caring for others and takes care of the rights of individuals as well as society. The life of Prophet Muhammad, r is the best role model in creating world peace atmosphere as He had ordered his followers and believers to realize peace in their societies. Islam guarantees peace even in war situation as it provides full protection and refuge to all those innocents who do not participate in the war. It is not unknown now that the contemporary world is witnessing events that souls hate and hearts alienate which caused racisms and populism and that their effects threatened communities and destroy firm foundations of peace. So in this contemporary perspective, the importance of research and writing about world peace increases subject to all of the studies levels, whether in universities or conferences especially in light of the biography of Holy Prophet Muhammad r. In this research, I will focus on the following points: Peace and its practical examples in of Holy Prophet Muhammad r. Explanation about the concept of peace and its practice in life of Holy Prophet Muhammad r. Clarifying the most prominent contemporary security challenges in the light of the Prophet's biography. Discussion on common misconceptions about Global peace matter and their correction in the sacred Biography of the Prophet Muhammad r

Evaluation of Biocontrol Potential of Endophytic Species of Penicillium Against Root Rotting Fungi and Root Knot Nematode

Endophytes are either bacteria or fungi that reside in the tissues of the plant without causing any apparent symptoms. Some endophytic microorganism may promote growth of plants, help in uptake of nutrients and increase the ability to bear environmental stresses, like salinity, drought, and reduce biotic stresses. During our study, plants were collected from different localities in Karachi Pakistan, like Memon Goth, Kathor, Gadap, Gharo, Malir and University of Karachi campus from which endophytic Penicillium were isolated. Out of the eightysamples of the plant, 14isolates of endophytic Penicillium isolated, (root, stem and leaves) from wild plants (Achyranthus aspera, Atriplex stocksii, Euphorbia hirta, Chorchorus tridens) and cultivated plant (Solanum melongena, Lycopersicon esculentum, Helianthus annuus, Azadirachta indica, Abelmoschus esculentus, Momordica charantia) Species of Penicillium identified as P. asperum, P. lilacinum, P. purpurogenum, P. nigricans, P. rugulosum, P. restrictum, P. duclauxi, P. citrinum, P. thomii, P. lividum and P. javanicum. Identification of selected isolates of Penicillium was also confirmed by using molecular biology tools. Antimicrobial activity of 14 endophytic isolates of different species of Penicillium tested against common fungi (root rotting) viz., F. oxysporum,, Fusarium solani, Macrophomina phaseolina and Rhizoctonia solani by dual culture plate assay. All EP isolates showed significant resultproduced by the inhibition zone. Nematicidal potential of cell free culture filtrates of endophytic Penicillium also has shown significant results. After 24 hour 50%nematicidal potential showed by P.purpurogenum (EP-3) while after 48 hours all other isolates showed 100% mortality. Culture filterates of endophytic Penicillium causedgrowth suppression ofbacteria Salmonella typhimurium, Bacillus subtilis,Escherichia coli and Staphylococcus aureus. Asconcentration increased biocontrol potential of culture filterates of EP increased as well. These outcomes show that endophytic Penicillium could be fullfil the need of discovering of new antibiotics. Culture filtrates of Penicillium also showed activity of fungicidal against root rotting fungal pathogens F.solani, R.solani, M.phaseolina, R.solani and F.oxysporum by making inhibitory zone. Cuture filterates of 60 µl/disc showed more effective results than 20 or 40 µl/disc. Fractionation of cell free culture filtrates of viable isolates of our Penicillium (EP), was made in solvents i.e chloroform and n-hexane and showed strong antibacterial and antifungal activity against above described pathogens. These results showed that secondry metabolites having compounds with strong antimicrobial potential. Secondary metabolites producing from endophytic Penicillium spp., offer an stimulating area of investigation for the encounter of novel antimicrobial compounds. Hexane fraction of mycelium of promising isolate EP-5 showed prescence of chemicals. In current research antagonistic potential of Penicillium was assessed against phytopathogens on sunflower (Helianthus annuus), chickpea (Cicer arietinum), tomato (Lycopersicon escolentum), mungbean (Vigna radiata) and okra (Abelmoschus esculentus) in field and screen house experiments. Inhibitory affects on F.oxysporum, R.solani, F.solani, and M.phaseolina showed by many endophytic Penicillium which causes healthy plant growth by improving plant length, fresh shoot weights in both type of experiments (Screen house and field). In some experiment, polyphenol and antioxidant activity also showed significant result which might be due to resistance produced by endophytes. Endophytic Penicillium treated plants produced fruits which is better in quality as compared to control.Endophytic Penicillium associated with healthy plants is a source of new bioactive metabolites, which could be exploited in plant protection and also in medicine.