تمھارے تیرِ نظر نے ہی مجھ کو مارا ہے
طنابیں کھینچ لے قاتل مجھے گوارا ہے
میں رازدارِ محبت ہوں آزمائو مجھے
کہ اب انگاروں پہ چلنا مجھے گوارا ہے
ہمیں جو بھولنا چاہو تو بھول سکتے ہو
ہماری یاد کہاں آخری سہارا ہے
تم اس کے صبر کی حد کیسے آزمائو گی
کہ جس کو تیرے بنا موت بھی خسارہ ہے
تمھاری جھیل سی آنکھوں میں رہنے آئی ہوں
اک ایسی جھیل نہ جس کا کوئی کنارہ ہے
حریمِ گل ہو ، محبت ہو یا فلک ہو فضاؔ
ترے سخن کے بنا کس کا اب گزارا ہے
Teens and children will need to be vaccinated in order to achieve herd immunity against COVID-19. Most parents are eager to have their teens immunized, whereas some are hesitant. Should teens be allowed to consent to immunization even if their parents’ objects? Vaccines are safe and effective, medically recommended, and good for both the individual and the community. Inspite of these powerful arguments, the law lags behind. Most parents want their teens to be immunized. Most vaccine-hesitant parents ultimately agree to vaccination. Furthermore, teens who get COVID generally have mild cases. For now, the law in most states does not permit teens to consent to the COVID-19 vaccine without parental permission.
The lignin degrading enzymes system of WRF has incredible catalytic property for oxidative bio-remediation of a lot of toxic contaminants and numerous other industrial applications. Immobilization facilitates the re-use of enzymes and making them industrially applicable and eco-nomical bio-catalysts. Schizophyllum commune IBL-06 was used for the production of lignin peroxidase (LiP) in pre-optimized solid state fermentation medium of corn stover. A high yield of LiP (1347.3 U/mL) was observed in crude extract. LiP was purified (5.65 folds) by a 4 steps protocol containing ammonium sulphate fractionation, dialysis, ion-exchange (DEAE-cellulose) and size exclusion chromatography. The 38 kDa single polypeptide S. commune IBL-06 LiP migrating as a single clear band on both native and SDS-PAGE gels. The purified enzyme was then immobilized on different support matrics such as chitosan, alginate, nylon membrane, PVA, agarose and PA gels with high immobilization efficiency and stability. Scanning electron microscopy (SEM) was performed for the verification of LiP attachment on support matrices. The maximum dye-decolorization (95.45%) potential was observed with chitosan and ca-alginate immobilized enzyme at 30°C without hemolytic toxicity. The chitosan beads-LiP retained approximately more than 70% activity after 3 frequent runs that gradually reduced to 35 % after 7th cycle of reusability. The immobilized LiP was found to show superior dye removal properties as compared to free LiP. Higher thermo stability, lower Km and high Vmax features of chitosan beads immobilizedLiP suggested its suitibility for variousbiotechnological and industrial applictaions.