ہر غم ہنسی کے پیچھے چھپانے سے میں رہا
اس بار زندگی کو نبھانے سے میں رہا
دیتا بھلے ہے تیری جفا کا دھواں یہ اب
پھر بھی چراغ ِ عشق بجھانے سے میں رہا
اب ماند پڑ گئی ہیں مری سب حرارتیں
اس شخص کو تو روز منانے سے میں رہا
جن میں ہے تیرے لمس کی خوشبو بسی ہوئی
اے یار! اُن خطوں کو جلانے سے میں رہا
گر تیری شرط ہے کہ یہ حل ہوں گی اشک سے
ان مشکلوں پہ اشک بہانے سے میں رہا
ان سب اذیتوں نے تو پاگل ہی کر دیا
ہوش اپنے اب ٹھکانے پہ لانے سے میں رہا
حساسیت سے چیخ کے یہ کہہ رہا ہوں میں
گر یہ زمانہ ہے تو زمانے سے میں رہا
مانا کہ فہد ؔزندگی بھی ہے بہت کٹھن
پر آسماں تو سر پہ اُٹھانے سے میں رہا
This study aims to determine the effect of body mass index (BMI), sleep patterns, working years, physical workload, mental workload, and work time on work fatigue on nurses at Hasanuddin University Hospital Makassar. This research is a quantitative study with a cross-sectional design with a sample size of 56 people. Sampling using a simple random sampling method. Data collection was carried out by means of a questionnaire. Bodyweight is measured by weight scales, body temperature is measured by microtoice, and physical workload is measured by a pulse oximeter, which is by looking at the pulse of the nurse before and after work. Data analysis in this study used the SPSS program. The results of this study indicate that there is an influence between Body Mass Index (BMI) on work fatigue (p = 0.001), there is an effect of sleep patterns on work fatigue (p = 0.019), there is an effect of tenure on work fatigue (p = 0.017), the relationship between physical workload and work fatigue (p = 0.000), there is an effect of mental workload on work fatigue (p = 0.000) and there is an effect of length of work on work fatigue (p = 0.017). The physical workload is the variable with the greatest influence on work fatigue on nurses at Hasanuddin University Hospital Makassar with Exp. (B) = 14,526. The results of this study concluded that there was a significant influence between the Body Mass Index (BMI), sleep patterns, years of work, physical workload, mental workload, and length of work for nurses at Hasanuddin University Hospital Makassar.
The present work is concerned with the determination of blood parameters, enzyme levels, blood chemistry and pesticide residues in the blood samples from different areas of Karachi in comparison with unexposed persons (i.e., control). Total 45 persons (exposed to pesticides) were taken in this study to assay the pesticides residues in their blood and also observed the morphological and chemical changes in the blood of affected persons. From 45 persons, only 20 persons were found positive and included in the study. Out of ten (10) standard pesticides five differentpesticides were identified in the blood of exposed persons such as cypermethrin, deltamethrin, polytrin-C, malathion and permethrin. The other standard pesticides such as DDE, DDT, match, diazinon and monocrotophos were not detected. In addition blood samples were also collected from ten (10) normal persons (not exposed to pesticides) for the determination of blood parameters, enzymes levels and blood chemistry. Regarding blood parameters approximately all the blood samples had significant variations i.e., (increase or decrease level) possibly due to the cumulative effect of pesticide residues e.g., Majid a young male aged 25 years had been exposed to pesticides for long time and therefore his TLC and platelet counts were found high in addition to low levels of RBC count, Hb, Hct and MCH which probably due to the excessive secretion of enzymes. Similarly, Ghulam Hussain another young male person aged only 08 years had low levels of RBC, Hb, Hct, platelet, MCV and MCH, which may be due to toxic effects of pesticides. In differential leukocyte count (DLC), granulocyte count was found in lower limits in 40 samples and showed normal limits in 05 samples. Lymphocyte count was xi found high in 31 samples. Low limit of lymphocyte was not found in any person. Similarly, monocyte count was found in high limits in 38 samples. Low monocyte count was not noted in any sample and the rest of the samples had normal limits of monocyte. Positive effects of pesticide residues were also observed on the enzyme levels. Regarding enzyme levels GPT, ALP, and ɣ - GT had higher levels in maximum number of samples. Cholinesterase was found high in 6 samples out of 45 samples and the highest was found in case of Farhan and Abdul Rahmanwhere as low limit of cholinesterase was noted in 15 samples out of total samples which may due to prolonged exposure of pesticides. GPT was found in high limits in all the samples. Also ɣ - GT and ALP found high in all the samples. Effect of pesticide residues were also observed on the blood chemistry. Regarding total bilirubin only one sample showed normal limit. The rest of the samples had raised total bilirubin and the highest was found in Javed a young male person aged 12 years. Similarly, in case of bilirubin direct all the blood samples showed increased level. The standard chromatograms of 10 different pesticides such as match, cypermethrin, deltamethrin, polytrin-C, diazinon, DDE, monocrotophos, DDT, malathion and permethrin were prepared on HPLC and their retention time (RT) was noted. Calculation was done for the quantity of pesticides residues from the peaks of chromatograms. Regarding pesticide residues, out of total 45 samples, only 20 persons were found positive for residues. The quantity of pesticide was determined by comparing xii the peak area of the standard pesticide with the sample peaks. After analysis, it was found that out of 20 samples deltamethrin and cypermethrin were found in noticeable amount in 6 samples. Also polytrin-C, malathion and permethrin were found in 2, 1 and 5 samples respectively. The pesticides such as match, diazinon, DDT and monocrotophoswere not detected in any sample.